Bacterial cellulose nanofiber-based films incorporating gelatin hydrolysate from tilapia skin: production, characterization and cytotoxicity assessment
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/56334 |
Resumo: | In this work, films based on bacterial cellulose nanofibers (BCNFs) incorporating gelatin hydrolysate (GH) from tilapia skin were produced. The effect of plasticizer (sorbitol or glycerol) and GH incorporation was evaluated on the physicalchemical and optical properties of films. BCNFs were produced using bacterial cellulose obtained from Hestrin and Schramm (HS) medium (BCNF-HS) or cashew apple juice (BCNF-CM), which was studied as an alternative to HS. Films with sorbitol showed the best properties and were selected for further characterization, using 40% (w/w) of BCNF-HS, 40% (w/w) of GH and 20% (w/w) of sorbitol (BCNF-HS-S-GH films). These films exhibited an antioxidant activity of 7.8 µmols Trolox Eq/g film, a water vapor permeability (WVP) of 1.6 g.mm/kPa.h.m2 and an Youngs modulus of 0.57 GPa. Films produced with BCNFs obtained from cashew apple juice revealed enhanced tensile strength, elongation at break, and thermal stability. Caco-2 cells viability after incubation with BCNF-based films incorporating GH was evaluated and showed non-cytotoxicity, reinforcing the safety of the developed materials and their potential use in food applications. |
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Bacterial cellulose nanofiber-based films incorporating gelatin hydrolysate from tilapia skin: production, characterization and cytotoxicity assessmentBiocompositeAntioxidantPlasticizerBioactive filmCashew apple juiceGelatin hydrolysateScience & TechnologyIn this work, films based on bacterial cellulose nanofibers (BCNFs) incorporating gelatin hydrolysate (GH) from tilapia skin were produced. The effect of plasticizer (sorbitol or glycerol) and GH incorporation was evaluated on the physicalchemical and optical properties of films. BCNFs were produced using bacterial cellulose obtained from Hestrin and Schramm (HS) medium (BCNF-HS) or cashew apple juice (BCNF-CM), which was studied as an alternative to HS. Films with sorbitol showed the best properties and were selected for further characterization, using 40% (w/w) of BCNF-HS, 40% (w/w) of GH and 20% (w/w) of sorbitol (BCNF-HS-S-GH films). These films exhibited an antioxidant activity of 7.8 µmols Trolox Eq/g film, a water vapor permeability (WVP) of 1.6 g.mm/kPa.h.m2 and an Youngs modulus of 0.57 GPa. Films produced with BCNFs obtained from cashew apple juice revealed enhanced tensile strength, elongation at break, and thermal stability. Caco-2 cells viability after incubation with BCNF-based films incorporating GH was evaluated and showed non-cytotoxicity, reinforcing the safety of the developed materials and their potential use in food applications.The authors would like to thank: Foundation of Support to the Scientific and Technological Development (FUNCAP, Brazil), Coordination for the Improvement of Higher Education Personnel (CAPES, Brazil), National Counsel of Technological and Scientific Development (CNPq, Brazil), Minho University (Braga, Portugal) and International Iberian Nanotechnology Laboratory (Braga, Portugal). This work was funded by research projects CNPq n 485465/2012-4, CNPq n 310368/2012-0 and CNPq n 476978/2013-0. Funding from Fundac¸a ˜o para a Cie ˆncia e Tecnologia through the project ‘‘Bacterial Cellulose: a platform for the development of bionanoproducts’’, under the bilateral program FCT/CAPES, is acknowledged. The authors acknowledge also the funding from QREN (‘‘Quadro de Referência Estratégica Nacional’’), ADI (‘‘Agência de Inovação’’) through the project Norte-070202-FEDER-038853, and BioTecNorte operation (NORTE01-0145-FEDER-000004) funded by the European Regional Development Fund under the scope of Norte2020—Programa Operacional Regional do Norte. This research was supported by Norte Regional Operational Program 2014–2020 (Norte2020) through the European Regional Development Fund (ERDF) Nanotechnology based functional solutions (NORTE-01-0145FEDER-000019).info:eu-repo/semantics/publishedVersionSpringerUniversidade do MinhoLima, Helder Levi SilvaGonçalves, CatarinaCerqueira, Miguel Ângelodo Nascimento, Elégenes SampaioGama, F. M.Rosa, Morsyleide F.Borges, Maria de FátimaPastrana, Lorenzo MiguelBrígida, Ana Iraidy Santa2018-102018-10-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/56334engLima, Helder Levi Silva; Gonçalves, Catarina; Cerqueira, Miguel Ângelo; do Nascimento, Elégenes Sampaio; Gama, F. M.; Rosa, Morsyleide F.; Borges, Maria de Fátima; Pastrana, Lorenzo Miguel; Brígida, Ana Iraidy Santa, Bacterial cellulose nanofiber-based films incorporating gelatin hydrolysate from tilapia skin: production, characterization and cytotoxicity assessment. Cellulose, 25(10), 6011-6029, 20180969-023910.1007/s10570-018-1983-0http://www.springer.com/chemistry/organic+chemistry/journal/10570info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:34:07Zoai:repositorium.sdum.uminho.pt:1822/56334Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:29:44.311526Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Bacterial cellulose nanofiber-based films incorporating gelatin hydrolysate from tilapia skin: production, characterization and cytotoxicity assessment |
title |
Bacterial cellulose nanofiber-based films incorporating gelatin hydrolysate from tilapia skin: production, characterization and cytotoxicity assessment |
spellingShingle |
Bacterial cellulose nanofiber-based films incorporating gelatin hydrolysate from tilapia skin: production, characterization and cytotoxicity assessment Lima, Helder Levi Silva Biocomposite Antioxidant Plasticizer Bioactive film Cashew apple juice Gelatin hydrolysate Science & Technology |
title_short |
Bacterial cellulose nanofiber-based films incorporating gelatin hydrolysate from tilapia skin: production, characterization and cytotoxicity assessment |
title_full |
Bacterial cellulose nanofiber-based films incorporating gelatin hydrolysate from tilapia skin: production, characterization and cytotoxicity assessment |
title_fullStr |
Bacterial cellulose nanofiber-based films incorporating gelatin hydrolysate from tilapia skin: production, characterization and cytotoxicity assessment |
title_full_unstemmed |
Bacterial cellulose nanofiber-based films incorporating gelatin hydrolysate from tilapia skin: production, characterization and cytotoxicity assessment |
title_sort |
Bacterial cellulose nanofiber-based films incorporating gelatin hydrolysate from tilapia skin: production, characterization and cytotoxicity assessment |
author |
Lima, Helder Levi Silva |
author_facet |
Lima, Helder Levi Silva Gonçalves, Catarina Cerqueira, Miguel Ângelo do Nascimento, Elégenes Sampaio Gama, F. M. Rosa, Morsyleide F. Borges, Maria de Fátima Pastrana, Lorenzo Miguel Brígida, Ana Iraidy Santa |
author_role |
author |
author2 |
Gonçalves, Catarina Cerqueira, Miguel Ângelo do Nascimento, Elégenes Sampaio Gama, F. M. Rosa, Morsyleide F. Borges, Maria de Fátima Pastrana, Lorenzo Miguel Brígida, Ana Iraidy Santa |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Lima, Helder Levi Silva Gonçalves, Catarina Cerqueira, Miguel Ângelo do Nascimento, Elégenes Sampaio Gama, F. M. Rosa, Morsyleide F. Borges, Maria de Fátima Pastrana, Lorenzo Miguel Brígida, Ana Iraidy Santa |
dc.subject.por.fl_str_mv |
Biocomposite Antioxidant Plasticizer Bioactive film Cashew apple juice Gelatin hydrolysate Science & Technology |
topic |
Biocomposite Antioxidant Plasticizer Bioactive film Cashew apple juice Gelatin hydrolysate Science & Technology |
description |
In this work, films based on bacterial cellulose nanofibers (BCNFs) incorporating gelatin hydrolysate (GH) from tilapia skin were produced. The effect of plasticizer (sorbitol or glycerol) and GH incorporation was evaluated on the physicalchemical and optical properties of films. BCNFs were produced using bacterial cellulose obtained from Hestrin and Schramm (HS) medium (BCNF-HS) or cashew apple juice (BCNF-CM), which was studied as an alternative to HS. Films with sorbitol showed the best properties and were selected for further characterization, using 40% (w/w) of BCNF-HS, 40% (w/w) of GH and 20% (w/w) of sorbitol (BCNF-HS-S-GH films). These films exhibited an antioxidant activity of 7.8 µmols Trolox Eq/g film, a water vapor permeability (WVP) of 1.6 g.mm/kPa.h.m2 and an Youngs modulus of 0.57 GPa. Films produced with BCNFs obtained from cashew apple juice revealed enhanced tensile strength, elongation at break, and thermal stability. Caco-2 cells viability after incubation with BCNF-based films incorporating GH was evaluated and showed non-cytotoxicity, reinforcing the safety of the developed materials and their potential use in food applications. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-10 2018-10-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/56334 |
url |
http://hdl.handle.net/1822/56334 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Lima, Helder Levi Silva; Gonçalves, Catarina; Cerqueira, Miguel Ângelo; do Nascimento, Elégenes Sampaio; Gama, F. M.; Rosa, Morsyleide F.; Borges, Maria de Fátima; Pastrana, Lorenzo Miguel; Brígida, Ana Iraidy Santa, Bacterial cellulose nanofiber-based films incorporating gelatin hydrolysate from tilapia skin: production, characterization and cytotoxicity assessment. Cellulose, 25(10), 6011-6029, 2018 0969-0239 10.1007/s10570-018-1983-0 http://www.springer.com/chemistry/organic+chemistry/journal/10570 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Springer |
publisher.none.fl_str_mv |
Springer |
dc.source.none.fl_str_mv |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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