Implementation of immature oocytes by cryopreservation technique and subsquent in vitro maturation and fertilization post-thawing

Detalhes bibliográficos
Autor(a) principal: Teixeira, Sofia Margarida Pontes
Data de Publicação: 2011
Tipo de documento: Dissertação
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.3/1241
Resumo: Dissertação de Mestrado em Engenharia Zootécnica.
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spelling Implementation of immature oocytes by cryopreservation technique and subsquent in vitro maturation and fertilization post-thawingImplementação da técnica de criopreservação de ovócitos imaturos com posterior descongelação, maturação e fecundação in vitroCattleCryopreservationEmbryoIn Vitro FertilizationVitrificationBovinosCriopreservaçãoEmbriologia AnimalFertilização Animal in vitroVitrificaçãoDissertação de Mestrado em Engenharia Zootécnica.The aim of this study was to cryopreserve by vitrification by propylene glycol (PROH) and dimethylsulfoxide (DMSO) immature bovine oocytes in straws and to investigate the effects of vitrification on post-thaw oocyte maturation and fertilization. A total of 983 cumulus oocyte complexes were obtained by follicle aspiration from 263 ovaries of cows slaughtered at a local slaughterhouse. Following selection, oocytes with compacted cumulus cells and evenly granulated ooplasm were vitrified using one of the two different solutions with a non vitrified group served as control. The first step vitrification solution contained 10% PROH while the second step solution contained 20% PROH+1M sucrose in a basic media used in group of PROH. Oocytes were matured in N-2-hidroxyethyl piperazine-N-2-ethanosulfonic acid (HEPES) buffered tissue culture medium (TCM) 199 supplemented with 10% FCS, 0.02 IU/ml FSH (Sigma), 1μg/ml E2 (Sigma), 0.15mg/ml Glutamine, 22μg/ml Na-Pyruvate, 10μg/ml Gentamycin, 10μg/ml Streptomycin and 10μg/ml Nistamycin. for 24 h at 38 ◦C in a humidified atmosphere of 5% CO2 in air. Oocytes were then fertilized and cultured. The numbers of developed embryos observed were 98 (70.1%), 73 (59.9%), and 80 (67.7%) in groups Control, PROH and DMSO, respectively, without co-culture. With co-culture were observed 141 (69.5%), 54 (61.7%), and 45 (49%), also in Control, PROH and DMSO. Developed embryos rate from control, without co-culture, was not statistically different when compared with PROH and DMSO (p< 0.05), but with co-culture present statistical differences with DMSO. However, better results were obtained in DMSP group compared to PROH, without co-culture. The lowest number of developed embryos was obtained in DMSO group, with co-culture. Immature bovine oocytes can be vitrified in straws, but success differs with the cryoprotectant and without co-culture.Silva, Joaquim Fernando Moreira daFaheem, Marwa SaidRepositório da Universidade dos AçoresTeixeira, Sofia Margarida Pontes2011-10-17T15:18:08Z2011-07-152011-07-15T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfhttp://hdl.handle.net/10400.3/1241enginfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2022-12-20T14:28:54Zoai:repositorio.uac.pt:10400.3/1241Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T16:24:07.072443Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Implementation of immature oocytes by cryopreservation technique and subsquent in vitro maturation and fertilization post-thawing
Implementação da técnica de criopreservação de ovócitos imaturos com posterior descongelação, maturação e fecundação in vitro
title Implementation of immature oocytes by cryopreservation technique and subsquent in vitro maturation and fertilization post-thawing
spellingShingle Implementation of immature oocytes by cryopreservation technique and subsquent in vitro maturation and fertilization post-thawing
Teixeira, Sofia Margarida Pontes
Cattle
Cryopreservation
Embryo
In Vitro Fertilization
Vitrification
Bovinos
Criopreservação
Embriologia Animal
Fertilização Animal in vitro
Vitrificação
title_short Implementation of immature oocytes by cryopreservation technique and subsquent in vitro maturation and fertilization post-thawing
title_full Implementation of immature oocytes by cryopreservation technique and subsquent in vitro maturation and fertilization post-thawing
title_fullStr Implementation of immature oocytes by cryopreservation technique and subsquent in vitro maturation and fertilization post-thawing
title_full_unstemmed Implementation of immature oocytes by cryopreservation technique and subsquent in vitro maturation and fertilization post-thawing
title_sort Implementation of immature oocytes by cryopreservation technique and subsquent in vitro maturation and fertilization post-thawing
author Teixeira, Sofia Margarida Pontes
author_facet Teixeira, Sofia Margarida Pontes
author_role author
dc.contributor.none.fl_str_mv Silva, Joaquim Fernando Moreira da
Faheem, Marwa Said
Repositório da Universidade dos Açores
dc.contributor.author.fl_str_mv Teixeira, Sofia Margarida Pontes
dc.subject.por.fl_str_mv Cattle
Cryopreservation
Embryo
In Vitro Fertilization
Vitrification
Bovinos
Criopreservação
Embriologia Animal
Fertilização Animal in vitro
Vitrificação
topic Cattle
Cryopreservation
Embryo
In Vitro Fertilization
Vitrification
Bovinos
Criopreservação
Embriologia Animal
Fertilização Animal in vitro
Vitrificação
description Dissertação de Mestrado em Engenharia Zootécnica.
publishDate 2011
dc.date.none.fl_str_mv 2011-10-17T15:18:08Z
2011-07-15
2011-07-15T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.3/1241
url http://hdl.handle.net/10400.3/1241
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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