A comparative study of tocopherols composition and antioxidant properties of in vivo and in vitro ectomycorrhizal fungi
Autor(a) principal: | |
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Data de Publicação: | 2011 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10198/3880 |
Resumo: | In aerobic organisms, the free radicals are constantly being produced during the normal cellular metabolism. The antioxidant properties of many organisms and particularly of wild mushrooms with their content in antioxidant compounds such as tocopherols, can detoxify potentially damaging forms of activated oxygen. Herein, a comparative study of tocopherols composition and antioxidant properties of in vivo (fruiting bodies) and in vitro (mycelia) ectomycorrhizal fungi: Paxillus involutus and Pisolithus arhizus. Tocopherols were determined by high performance liquid chromatography (HPLC) coupled to a fluorescence detector. The antioxidant properties were studied in terms of DPPH radical-scavenging activity, reducing power and inhibition of β-carotene bleaching. Fruiting bodies revealed the highest antioxidant properties, including scavenging effects on free radicals (EC50 = 0.61 and 0.56 mg/ml) and inhibition of lipid peroxidation capacity (EC50 = 0.40 and 0.24 mg/ml for Paxillus involutus and Pisolithus arhizus, respectively), than mycelia produced in vitro cultures. Nevertheless, mycelia revealed higher levels of total tocopherols than fruiting bodies, and particularly Pisolithus arhizus mycelium proved to be a powerful source of γ-tocopherol (154.39 μg/g dry weight). |
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A comparative study of tocopherols composition and antioxidant properties of in vivo and in vitro ectomycorrhizal fungiPaxillus involutusPisolithus arhizusFruiting bodies/myceliaTocopherolsHPLC-fluorescenceAntioxidant activityIn aerobic organisms, the free radicals are constantly being produced during the normal cellular metabolism. The antioxidant properties of many organisms and particularly of wild mushrooms with their content in antioxidant compounds such as tocopherols, can detoxify potentially damaging forms of activated oxygen. Herein, a comparative study of tocopherols composition and antioxidant properties of in vivo (fruiting bodies) and in vitro (mycelia) ectomycorrhizal fungi: Paxillus involutus and Pisolithus arhizus. Tocopherols were determined by high performance liquid chromatography (HPLC) coupled to a fluorescence detector. The antioxidant properties were studied in terms of DPPH radical-scavenging activity, reducing power and inhibition of β-carotene bleaching. Fruiting bodies revealed the highest antioxidant properties, including scavenging effects on free radicals (EC50 = 0.61 and 0.56 mg/ml) and inhibition of lipid peroxidation capacity (EC50 = 0.40 and 0.24 mg/ml for Paxillus involutus and Pisolithus arhizus, respectively), than mycelia produced in vitro cultures. Nevertheless, mycelia revealed higher levels of total tocopherols than fruiting bodies, and particularly Pisolithus arhizus mycelium proved to be a powerful source of γ-tocopherol (154.39 μg/g dry weight).ElsevierBiblioteca Digital do IPBReis, Filipa S.Ferreira, Isabel C.F.R.Barros, LillianMartins, Anabela2011-04-01T10:49:20Z20112011-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10198/3880engReis, Filipa S.; Ferreira, Isabel C.F.R.; Barros, Lillian; Martins, Anabela (2011). A comparative study of tocopherols composition and antioxidant properties of in vivo and in vitro ectomycorrhizal fungi. LWT - Food Science and Technology. ISSN 0023-6438. 44:4, p. 820-8240023-643810.1016/j.lwt.2010.11.033info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-21T10:08:22Zoai:bibliotecadigital.ipb.pt:10198/3880Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T22:56:09.249407Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
A comparative study of tocopherols composition and antioxidant properties of in vivo and in vitro ectomycorrhizal fungi |
title |
A comparative study of tocopherols composition and antioxidant properties of in vivo and in vitro ectomycorrhizal fungi |
spellingShingle |
A comparative study of tocopherols composition and antioxidant properties of in vivo and in vitro ectomycorrhizal fungi Reis, Filipa S. Paxillus involutus Pisolithus arhizus Fruiting bodies/mycelia Tocopherols HPLC-fluorescence Antioxidant activity |
title_short |
A comparative study of tocopherols composition and antioxidant properties of in vivo and in vitro ectomycorrhizal fungi |
title_full |
A comparative study of tocopherols composition and antioxidant properties of in vivo and in vitro ectomycorrhizal fungi |
title_fullStr |
A comparative study of tocopherols composition and antioxidant properties of in vivo and in vitro ectomycorrhizal fungi |
title_full_unstemmed |
A comparative study of tocopherols composition and antioxidant properties of in vivo and in vitro ectomycorrhizal fungi |
title_sort |
A comparative study of tocopherols composition and antioxidant properties of in vivo and in vitro ectomycorrhizal fungi |
author |
Reis, Filipa S. |
author_facet |
Reis, Filipa S. Ferreira, Isabel C.F.R. Barros, Lillian Martins, Anabela |
author_role |
author |
author2 |
Ferreira, Isabel C.F.R. Barros, Lillian Martins, Anabela |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
Biblioteca Digital do IPB |
dc.contributor.author.fl_str_mv |
Reis, Filipa S. Ferreira, Isabel C.F.R. Barros, Lillian Martins, Anabela |
dc.subject.por.fl_str_mv |
Paxillus involutus Pisolithus arhizus Fruiting bodies/mycelia Tocopherols HPLC-fluorescence Antioxidant activity |
topic |
Paxillus involutus Pisolithus arhizus Fruiting bodies/mycelia Tocopherols HPLC-fluorescence Antioxidant activity |
description |
In aerobic organisms, the free radicals are constantly being produced during the normal cellular metabolism. The antioxidant properties of many organisms and particularly of wild mushrooms with their content in antioxidant compounds such as tocopherols, can detoxify potentially damaging forms of activated oxygen. Herein, a comparative study of tocopherols composition and antioxidant properties of in vivo (fruiting bodies) and in vitro (mycelia) ectomycorrhizal fungi: Paxillus involutus and Pisolithus arhizus. Tocopherols were determined by high performance liquid chromatography (HPLC) coupled to a fluorescence detector. The antioxidant properties were studied in terms of DPPH radical-scavenging activity, reducing power and inhibition of β-carotene bleaching. Fruiting bodies revealed the highest antioxidant properties, including scavenging effects on free radicals (EC50 = 0.61 and 0.56 mg/ml) and inhibition of lipid peroxidation capacity (EC50 = 0.40 and 0.24 mg/ml for Paxillus involutus and Pisolithus arhizus, respectively), than mycelia produced in vitro cultures. Nevertheless, mycelia revealed higher levels of total tocopherols than fruiting bodies, and particularly Pisolithus arhizus mycelium proved to be a powerful source of γ-tocopherol (154.39 μg/g dry weight). |
publishDate |
2011 |
dc.date.none.fl_str_mv |
2011-04-01T10:49:20Z 2011 2011-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10198/3880 |
url |
http://hdl.handle.net/10198/3880 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Reis, Filipa S.; Ferreira, Isabel C.F.R.; Barros, Lillian; Martins, Anabela (2011). A comparative study of tocopherols composition and antioxidant properties of in vivo and in vitro ectomycorrhizal fungi. LWT - Food Science and Technology. ISSN 0023-6438. 44:4, p. 820-824 0023-6438 10.1016/j.lwt.2010.11.033 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799135168412254208 |