Chronic experimental model of TNBS-induced colitis to study inflammatory bowel disease

Detalhes bibliográficos
Autor(a) principal: Silva, Inês
Data de Publicação: 2022
Outros Autores: Solas, João, Pinto, Rui, Mateus, Vanessa
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.21/14599
Resumo: IDI&CA_IPL/2019/COLITiS_ESTeSL
id RCAP_19bfccf222a5fa15b5413abfe3615a59
oai_identifier_str oai:repositorio.ipl.pt:10400.21/14599
network_acronym_str RCAP
network_name_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository_id_str 7160
spelling Chronic experimental model of TNBS-induced colitis to study inflammatory bowel diseaseInflammatory bowel diseaseTNBS-induced colitisChronic animal modelIDI&CA_IPL/2019/COLITiS_ESTeSLFCT_UIDB/05608/2020FCT_UIDP/05608/2020IDI&CA_IPL/2019/COLITiS_ESTeSLFCT_UIDB/05608/2020. FCT_UIDP/05608/2020.Background: Inflammatory bowel disease (IBD) is a world healthcare problem. In order to evaluate the effect of new pharmacological approaches for IBD, we aim to develop and validate chronic trinitrobenzene sulfonic acid (TNBS)-induced colitis in mice. Methods: Experimental colitis was induced by the rectal administration of multiple doses of TNBS in female CD-1 mice. The protocol was performed with six experimental groups, depending on the TNBS administration frequency, and two control groups (sham and ethanol groups). Results: The survival rate was 73.3% in the first three weeks and, from week 4 until the end of the experimental protocol, the mice’s survival remained unaltered at 70.9%. Fecal hemoglobin presented a progressive increase until week 4 (5.8 ± 0.3 µmol Hg/g feces, p < 0.0001) compared with the ethanol group, with no statistical differences to week 6. The highest level of tumor necrosis factor-α was observed on week 3; however, after week 4, a slight decrease in tumor necrosis factor-α concentration was verified, and the level was maintained until week 6 (71.3 ± 3.3 pg/mL and 72.7 ± 3.6 pg/mL, respectively). Conclusions: These findings allowed the verification of a stable pattern of clinical and inflammation signs after week 4, suggesting that the chronic model of TNBS-induced colitis develops in 4 weeks.MDPIRCIPLSilva, InêsSolas, JoãoPinto, RuiMateus, Vanessa2022-04-27T16:12:52Z2022-042022-04-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.21/14599engSilva IJ, Solas J, Pinto R, Mateus V. Chronic experimental model of TNBS-induced colitis to study inflammatory bowel disease. Int J Mol Sci. 2022;23(9):4739.10.3390/ijms23094739info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-08-03T10:11:01Zoai:repositorio.ipl.pt:10400.21/14599Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T20:22:22.638748Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Chronic experimental model of TNBS-induced colitis to study inflammatory bowel disease
title Chronic experimental model of TNBS-induced colitis to study inflammatory bowel disease
spellingShingle Chronic experimental model of TNBS-induced colitis to study inflammatory bowel disease
Silva, Inês
Inflammatory bowel disease
TNBS-induced colitis
Chronic animal model
IDI&CA_IPL/2019/COLITiS_ESTeSL
FCT_UIDB/05608/2020
FCT_UIDP/05608/2020
title_short Chronic experimental model of TNBS-induced colitis to study inflammatory bowel disease
title_full Chronic experimental model of TNBS-induced colitis to study inflammatory bowel disease
title_fullStr Chronic experimental model of TNBS-induced colitis to study inflammatory bowel disease
title_full_unstemmed Chronic experimental model of TNBS-induced colitis to study inflammatory bowel disease
title_sort Chronic experimental model of TNBS-induced colitis to study inflammatory bowel disease
author Silva, Inês
author_facet Silva, Inês
Solas, João
Pinto, Rui
Mateus, Vanessa
author_role author
author2 Solas, João
Pinto, Rui
Mateus, Vanessa
author2_role author
author
author
dc.contributor.none.fl_str_mv RCIPL
dc.contributor.author.fl_str_mv Silva, Inês
Solas, João
Pinto, Rui
Mateus, Vanessa
dc.subject.por.fl_str_mv Inflammatory bowel disease
TNBS-induced colitis
Chronic animal model
IDI&CA_IPL/2019/COLITiS_ESTeSL
FCT_UIDB/05608/2020
FCT_UIDP/05608/2020
topic Inflammatory bowel disease
TNBS-induced colitis
Chronic animal model
IDI&CA_IPL/2019/COLITiS_ESTeSL
FCT_UIDB/05608/2020
FCT_UIDP/05608/2020
description IDI&CA_IPL/2019/COLITiS_ESTeSL
publishDate 2022
dc.date.none.fl_str_mv 2022-04-27T16:12:52Z
2022-04
2022-04-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.21/14599
url http://hdl.handle.net/10400.21/14599
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Silva IJ, Solas J, Pinto R, Mateus V. Chronic experimental model of TNBS-induced colitis to study inflammatory bowel disease. Int J Mol Sci. 2022;23(9):4739.
10.3390/ijms23094739
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
_version_ 1799133495513055232

Registros relacionados