Evaluation of the best method to assess antibiotic potentiation by phytochemicals against staphylococcus aureus
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/33166 |
Resumo: | The increasing occurrence of bacterial resistance to antibiotics has now reached a critical level. Finding antibiotic coadjuvants capable to inhibit the bacterial resistance mechanisms would be a valuable mid-term solution, until new classes of antibiotics are discovered. Selected plant alkaloids were combined with 5 antibiotics against 10 Staphylococcus aureus strains, including strains expressing distinct efflux pumps and methicillin-resistant S. aureus strains. The efficacy of each combination was assessed using the microdilution checkerboard, time-kill, Etest, and disc diffusion methods. The cytotoxicity of the alkaloids was evaluated in a mouse fibroblast cell line. Potentiation was obtained in 6% of all 190 combinations, especially with the combination of: ciprofloxacin with reserpine (RES), pyrrolidine (PYR), and quinine (QUIN); tetracycline with RES; and erythromycin with PYR. The highest cytotoxicity values were found for QUIN (half maximal inhibitory concentration [IC50] = 25 ± 2.2 mg/L) and theophylline (IC50 = 100 ± 4.7 mg/L). |
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Evaluation of the best method to assess antibiotic potentiation by phytochemicals against staphylococcus aureusAlkaloidsCo-therapyEfflux pumpPotentiationResistance-modifying agentsScience & TechnologyThe increasing occurrence of bacterial resistance to antibiotics has now reached a critical level. Finding antibiotic coadjuvants capable to inhibit the bacterial resistance mechanisms would be a valuable mid-term solution, until new classes of antibiotics are discovered. Selected plant alkaloids were combined with 5 antibiotics against 10 Staphylococcus aureus strains, including strains expressing distinct efflux pumps and methicillin-resistant S. aureus strains. The efficacy of each combination was assessed using the microdilution checkerboard, time-kill, Etest, and disc diffusion methods. The cytotoxicity of the alkaloids was evaluated in a mouse fibroblast cell line. Potentiation was obtained in 6% of all 190 combinations, especially with the combination of: ciprofloxacin with reserpine (RES), pyrrolidine (PYR), and quinine (QUIN); tetracycline with RES; and erythromycin with PYR. The highest cytotoxicity values were found for QUIN (half maximal inhibitory concentration [IC50] = 25 ± 2.2 mg/L) and theophylline (IC50 = 100 ± 4.7 mg/L).The authors are very grateful to Professor Simon Gibbons (Department of Pharmaceutical and Biological Chemistry, The School of Pharmacy, UCL School of Pharmacy, London) for providing some of the bacterial strains. This work was supported by Operational Programme for Competitiveness Factors - COMPETE and by FCT Portuguese Foundation for Science and Technology through Projects Bioresist - PTDC/EBB-EBI/105085/2008; Phytodisinfectants - PTDC/DTP-SAP/1078/2012 and the PhD grants awarded to Ana Abreu (SFRH/BD/84393/2012) and Anabela Borges (SFRH/BD/63398/2009).ElsevierUniversidade do MinhoAbreu, Ana CristinaSerra, Sofia CristinaBorges, AnabelaSaavedra, Maria JoséSalgado, A. J.Simões, Manuel2014-062014-06-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/33166eng0732-889310.1016/j.diagmicrobio.2014.03.00224717959http://www.springer.cominfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:06:53Zoai:repositorium.sdum.uminho.pt:1822/33166Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:57:41.948347Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Evaluation of the best method to assess antibiotic potentiation by phytochemicals against staphylococcus aureus |
title |
Evaluation of the best method to assess antibiotic potentiation by phytochemicals against staphylococcus aureus |
spellingShingle |
Evaluation of the best method to assess antibiotic potentiation by phytochemicals against staphylococcus aureus Abreu, Ana Cristina Alkaloids Co-therapy Efflux pump Potentiation Resistance-modifying agents Science & Technology |
title_short |
Evaluation of the best method to assess antibiotic potentiation by phytochemicals against staphylococcus aureus |
title_full |
Evaluation of the best method to assess antibiotic potentiation by phytochemicals against staphylococcus aureus |
title_fullStr |
Evaluation of the best method to assess antibiotic potentiation by phytochemicals against staphylococcus aureus |
title_full_unstemmed |
Evaluation of the best method to assess antibiotic potentiation by phytochemicals against staphylococcus aureus |
title_sort |
Evaluation of the best method to assess antibiotic potentiation by phytochemicals against staphylococcus aureus |
author |
Abreu, Ana Cristina |
author_facet |
Abreu, Ana Cristina Serra, Sofia Cristina Borges, Anabela Saavedra, Maria José Salgado, A. J. Simões, Manuel |
author_role |
author |
author2 |
Serra, Sofia Cristina Borges, Anabela Saavedra, Maria José Salgado, A. J. Simões, Manuel |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Abreu, Ana Cristina Serra, Sofia Cristina Borges, Anabela Saavedra, Maria José Salgado, A. J. Simões, Manuel |
dc.subject.por.fl_str_mv |
Alkaloids Co-therapy Efflux pump Potentiation Resistance-modifying agents Science & Technology |
topic |
Alkaloids Co-therapy Efflux pump Potentiation Resistance-modifying agents Science & Technology |
description |
The increasing occurrence of bacterial resistance to antibiotics has now reached a critical level. Finding antibiotic coadjuvants capable to inhibit the bacterial resistance mechanisms would be a valuable mid-term solution, until new classes of antibiotics are discovered. Selected plant alkaloids were combined with 5 antibiotics against 10 Staphylococcus aureus strains, including strains expressing distinct efflux pumps and methicillin-resistant S. aureus strains. The efficacy of each combination was assessed using the microdilution checkerboard, time-kill, Etest, and disc diffusion methods. The cytotoxicity of the alkaloids was evaluated in a mouse fibroblast cell line. Potentiation was obtained in 6% of all 190 combinations, especially with the combination of: ciprofloxacin with reserpine (RES), pyrrolidine (PYR), and quinine (QUIN); tetracycline with RES; and erythromycin with PYR. The highest cytotoxicity values were found for QUIN (half maximal inhibitory concentration [IC50] = 25 ± 2.2 mg/L) and theophylline (IC50 = 100 ± 4.7 mg/L). |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-06 2014-06-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/33166 |
url |
http://hdl.handle.net/1822/33166 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
0732-8893 10.1016/j.diagmicrobio.2014.03.002 24717959 http://www.springer.com |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1799132365530857472 |