Injectable and magnetic responsive hydrogels with bioinspired ordered structures
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/68334 |
Resumo: | Injectable hydrogels are particularly interesting for applications in minimally invasive tissue engineering and regenerative medicine strategies. However, the typical isotropic microstructure of these biomaterials limits their potential for the regeneration of ordered tissues. In the present work, we decorated rod-shaped cellulose nanocrystals with magnetic nanoparticles and coated these with polydopamine and polyethylene glycol polymer brushes to obtain chemical and colloidal stable nanoparticles. Then, these nanoparticles (0.1-0.5 wt %) were incorporated within gelatin hydrogels, creating injectable and magnetically responsive materials with potential for various biomedical applications. Nanoparticle alignment within the hydrogel matrix was achieved under exposure to uniform low magnetic fields (108 mT), resulting in biomaterials with directional microstructure and anisotropic mechanical properties. The biological performance of these nanocomposite hydrogels was studied using adipose tissue derived human stem cells. Cells encapsulated in the nanocomposite hydrogels showed high rates of viability demonstrating that the nanocomposite biomaterials are not cytotoxic. Remarkably, the microstructural patterns stemming from nanoparticle alignment induced the directional growth of seeded and, to a lower extent, encapsulated cells in the hydrogels, suggesting that this injectable system might find application in both cellular and acellular strategies targeting the regeneration of anisotropic tissues. |
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Injectable and magnetic responsive hydrogels with bioinspired ordered structuresAnisotropic hydrogelsMagnetic alignmentMagnetic nanoparticlesOrdered tissuesScience & TechnologyInjectable hydrogels are particularly interesting for applications in minimally invasive tissue engineering and regenerative medicine strategies. However, the typical isotropic microstructure of these biomaterials limits their potential for the regeneration of ordered tissues. In the present work, we decorated rod-shaped cellulose nanocrystals with magnetic nanoparticles and coated these with polydopamine and polyethylene glycol polymer brushes to obtain chemical and colloidal stable nanoparticles. Then, these nanoparticles (0.1-0.5 wt %) were incorporated within gelatin hydrogels, creating injectable and magnetically responsive materials with potential for various biomedical applications. Nanoparticle alignment within the hydrogel matrix was achieved under exposure to uniform low magnetic fields (108 mT), resulting in biomaterials with directional microstructure and anisotropic mechanical properties. The biological performance of these nanocomposite hydrogels was studied using adipose tissue derived human stem cells. Cells encapsulated in the nanocomposite hydrogels showed high rates of viability demonstrating that the nanocomposite biomaterials are not cytotoxic. Remarkably, the microstructural patterns stemming from nanoparticle alignment induced the directional growth of seeded and, to a lower extent, encapsulated cells in the hydrogels, suggesting that this injectable system might find application in both cellular and acellular strategies targeting the regeneration of anisotropic tissues.Fundação para a Ciência e a Tecnologia for SFRH/BPD/112459/2015 (RD), EU’s H2020 programme for Marie Skłodowska-Curie grant agreement 706996 and for European Research Council grant agreement 772817 - MagTendon, project RECOGNIZE (UTAPICDT/CTM-BIO/0023/2014), project FOOD4CELLS (PTDC/CTM-BIO/4706/2014 - POCI-01- 0145-FEDER 016716) (PB), and project NORTE-01-0145-FEDER-000021.American Chemical SocietyUniversidade do MinhoAraújo-Custódio, SandraGomez-Florit, ManuelTomás, Ana R.Mendes, Bárbara B.Babo, Pedro Miguel SousaMithieux, Suzanne MWeiss, AnthonyDomingues, Rui Miguel AndradeReis, R. L.Gomes, Manuela E.2019-022019-02-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/68334engAraújo-Custódio S., Gómez-Florit M., Tomás A. R., Mendes B. B., Babo P. S., Mithieux S. M., Weiss A. S., Domingues R. M. A., Reis R. L., Gomes M. E. Injectable and Magnetic Responsive Hydrogels with Bioinspired Ordered Structures, ACS Biomaterials Science and Engineering, Vol. 5, Issue 3, pp. 1392-1404, doi:10.1021/acsbiomaterials.8b01179, 20192373-987810.1021/acsbiomaterials.8b01179https://doi.org/10.1021/acsbiomaterials.8b01179info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:29:22Zoai:repositorium.sdum.uminho.pt:1822/68334Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:24:20.784499Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Injectable and magnetic responsive hydrogels with bioinspired ordered structures |
title |
Injectable and magnetic responsive hydrogels with bioinspired ordered structures |
spellingShingle |
Injectable and magnetic responsive hydrogels with bioinspired ordered structures Araújo-Custódio, Sandra Anisotropic hydrogels Magnetic alignment Magnetic nanoparticles Ordered tissues Science & Technology |
title_short |
Injectable and magnetic responsive hydrogels with bioinspired ordered structures |
title_full |
Injectable and magnetic responsive hydrogels with bioinspired ordered structures |
title_fullStr |
Injectable and magnetic responsive hydrogels with bioinspired ordered structures |
title_full_unstemmed |
Injectable and magnetic responsive hydrogels with bioinspired ordered structures |
title_sort |
Injectable and magnetic responsive hydrogels with bioinspired ordered structures |
author |
Araújo-Custódio, Sandra |
author_facet |
Araújo-Custódio, Sandra Gomez-Florit, Manuel Tomás, Ana R. Mendes, Bárbara B. Babo, Pedro Miguel Sousa Mithieux, Suzanne M Weiss, Anthony Domingues, Rui Miguel Andrade Reis, R. L. Gomes, Manuela E. |
author_role |
author |
author2 |
Gomez-Florit, Manuel Tomás, Ana R. Mendes, Bárbara B. Babo, Pedro Miguel Sousa Mithieux, Suzanne M Weiss, Anthony Domingues, Rui Miguel Andrade Reis, R. L. Gomes, Manuela E. |
author2_role |
author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Araújo-Custódio, Sandra Gomez-Florit, Manuel Tomás, Ana R. Mendes, Bárbara B. Babo, Pedro Miguel Sousa Mithieux, Suzanne M Weiss, Anthony Domingues, Rui Miguel Andrade Reis, R. L. Gomes, Manuela E. |
dc.subject.por.fl_str_mv |
Anisotropic hydrogels Magnetic alignment Magnetic nanoparticles Ordered tissues Science & Technology |
topic |
Anisotropic hydrogels Magnetic alignment Magnetic nanoparticles Ordered tissues Science & Technology |
description |
Injectable hydrogels are particularly interesting for applications in minimally invasive tissue engineering and regenerative medicine strategies. However, the typical isotropic microstructure of these biomaterials limits their potential for the regeneration of ordered tissues. In the present work, we decorated rod-shaped cellulose nanocrystals with magnetic nanoparticles and coated these with polydopamine and polyethylene glycol polymer brushes to obtain chemical and colloidal stable nanoparticles. Then, these nanoparticles (0.1-0.5 wt %) were incorporated within gelatin hydrogels, creating injectable and magnetically responsive materials with potential for various biomedical applications. Nanoparticle alignment within the hydrogel matrix was achieved under exposure to uniform low magnetic fields (108 mT), resulting in biomaterials with directional microstructure and anisotropic mechanical properties. The biological performance of these nanocomposite hydrogels was studied using adipose tissue derived human stem cells. Cells encapsulated in the nanocomposite hydrogels showed high rates of viability demonstrating that the nanocomposite biomaterials are not cytotoxic. Remarkably, the microstructural patterns stemming from nanoparticle alignment induced the directional growth of seeded and, to a lower extent, encapsulated cells in the hydrogels, suggesting that this injectable system might find application in both cellular and acellular strategies targeting the regeneration of anisotropic tissues. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-02 2019-02-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/68334 |
url |
http://hdl.handle.net/1822/68334 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Araújo-Custódio S., Gómez-Florit M., Tomás A. R., Mendes B. B., Babo P. S., Mithieux S. M., Weiss A. S., Domingues R. M. A., Reis R. L., Gomes M. E. Injectable and Magnetic Responsive Hydrogels with Bioinspired Ordered Structures, ACS Biomaterials Science and Engineering, Vol. 5, Issue 3, pp. 1392-1404, doi:10.1021/acsbiomaterials.8b01179, 2019 2373-9878 10.1021/acsbiomaterials.8b01179 https://doi.org/10.1021/acsbiomaterials.8b01179 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
American Chemical Society |
publisher.none.fl_str_mv |
American Chemical Society |
dc.source.none.fl_str_mv |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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