Ofloxacin determination in urine, serum and pharmaceuticals using an automatic flow potentiometric system

Detalhes bibliográficos
Autor(a) principal: Pimenta, Adriana M.
Data de Publicação: 2013
Outros Autores: Souto, M. Renata S., Catarino, Rita I. L., Leal, M. Fernanda C., Lima, José L. F. Costa
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.14/34101
Resumo: An automatic system was developed to determine ofloxacin in biological fluids and pharmaceutical formulations. Drug detection was carried out by a potentiometric membrane sensor based on [bis(trifluoromethyl)phenyl]borate as molecular-recognition material. The tubular shaped detector system was solidly attached to the manifold, creating a high-throughput stable setup (50 samples per hour) appropriate for routine antibiotic assessment. Under the optimized flow conditions, the sensor displayed a mean detection limit of 1 × 10 -5 M, a linear response over the concentrations of 2 × 10 -5 to 5 × 10 -3 M (slope of 57.4 mV decade -1 ) and a wide working pH range (2.1-6.6). The procedure was successfully applied to ofloxacin analysis in pharmaceuticals (relative deviation lower than 6%) and biological fluids at levels usually found after drug administration of clinical doses (recoveries between 91 and 106%). No significant interference from common excipients found in commercial formulations and inorganic ions usually present in biological fluids was noticed.
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spelling Ofloxacin determination in urine, serum and pharmaceuticals using an automatic flow potentiometric systemAutomatic determinationOfloxacinPharmaceuticalsSerumUrineAn automatic system was developed to determine ofloxacin in biological fluids and pharmaceutical formulations. Drug detection was carried out by a potentiometric membrane sensor based on [bis(trifluoromethyl)phenyl]borate as molecular-recognition material. The tubular shaped detector system was solidly attached to the manifold, creating a high-throughput stable setup (50 samples per hour) appropriate for routine antibiotic assessment. Under the optimized flow conditions, the sensor displayed a mean detection limit of 1 × 10 -5 M, a linear response over the concentrations of 2 × 10 -5 to 5 × 10 -3 M (slope of 57.4 mV decade -1 ) and a wide working pH range (2.1-6.6). The procedure was successfully applied to ofloxacin analysis in pharmaceuticals (relative deviation lower than 6%) and biological fluids at levels usually found after drug administration of clinical doses (recoveries between 91 and 106%). No significant interference from common excipients found in commercial formulations and inorganic ions usually present in biological fluids was noticed.Veritati - Repositório Institucional da Universidade Católica PortuguesaPimenta, Adriana M.Souto, M. Renata S.Catarino, Rita I. L.Leal, M. Fernanda C.Lima, José L. F. Costa2021-07-06T19:38:23Z20132013-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.14/34101eng0910-634010.2116/analsci.29.8938488493418124025573info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-12T17:39:34Zoai:repositorio.ucp.pt:10400.14/34101Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:27:35.486188Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Ofloxacin determination in urine, serum and pharmaceuticals using an automatic flow potentiometric system
title Ofloxacin determination in urine, serum and pharmaceuticals using an automatic flow potentiometric system
spellingShingle Ofloxacin determination in urine, serum and pharmaceuticals using an automatic flow potentiometric system
Pimenta, Adriana M.
Automatic determination
Ofloxacin
Pharmaceuticals
Serum
Urine
title_short Ofloxacin determination in urine, serum and pharmaceuticals using an automatic flow potentiometric system
title_full Ofloxacin determination in urine, serum and pharmaceuticals using an automatic flow potentiometric system
title_fullStr Ofloxacin determination in urine, serum and pharmaceuticals using an automatic flow potentiometric system
title_full_unstemmed Ofloxacin determination in urine, serum and pharmaceuticals using an automatic flow potentiometric system
title_sort Ofloxacin determination in urine, serum and pharmaceuticals using an automatic flow potentiometric system
author Pimenta, Adriana M.
author_facet Pimenta, Adriana M.
Souto, M. Renata S.
Catarino, Rita I. L.
Leal, M. Fernanda C.
Lima, José L. F. Costa
author_role author
author2 Souto, M. Renata S.
Catarino, Rita I. L.
Leal, M. Fernanda C.
Lima, José L. F. Costa
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Veritati - Repositório Institucional da Universidade Católica Portuguesa
dc.contributor.author.fl_str_mv Pimenta, Adriana M.
Souto, M. Renata S.
Catarino, Rita I. L.
Leal, M. Fernanda C.
Lima, José L. F. Costa
dc.subject.por.fl_str_mv Automatic determination
Ofloxacin
Pharmaceuticals
Serum
Urine
topic Automatic determination
Ofloxacin
Pharmaceuticals
Serum
Urine
description An automatic system was developed to determine ofloxacin in biological fluids and pharmaceutical formulations. Drug detection was carried out by a potentiometric membrane sensor based on [bis(trifluoromethyl)phenyl]borate as molecular-recognition material. The tubular shaped detector system was solidly attached to the manifold, creating a high-throughput stable setup (50 samples per hour) appropriate for routine antibiotic assessment. Under the optimized flow conditions, the sensor displayed a mean detection limit of 1 × 10 -5 M, a linear response over the concentrations of 2 × 10 -5 to 5 × 10 -3 M (slope of 57.4 mV decade -1 ) and a wide working pH range (2.1-6.6). The procedure was successfully applied to ofloxacin analysis in pharmaceuticals (relative deviation lower than 6%) and biological fluids at levels usually found after drug administration of clinical doses (recoveries between 91 and 106%). No significant interference from common excipients found in commercial formulations and inorganic ions usually present in biological fluids was noticed.
publishDate 2013
dc.date.none.fl_str_mv 2013
2013-01-01T00:00:00Z
2021-07-06T19:38:23Z
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dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.14/34101
url http://hdl.handle.net/10400.14/34101
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 0910-6340
10.2116/analsci.29.893
84884934181
24025573
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