Expression and purification of proteins involved in the mitochondrial electron transport chain

Detalhes bibliográficos
Autor(a) principal: Silva, José Pedro Malanho da
Data de Publicação: 2015
Tipo de documento: Dissertação
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/43590
Resumo: NADH: ubiquinone oxidoredutase is one of the most intricate enzymes. It is part of the respiratory chains and is present in several organisms. This enzyme couples the transfer of two electron, from NADH to ubiquinone, and translocate ions across the membrane establishing a transmembrane difference of electrochemical potential. In this work, we present the initial experimental steps to express and purify a subunit from this intricate enzyme, which is directly related with the translocation of ions (i.e. NuoL). The objective was to characterize the subunit reconstituted in membrane-mimicking nanoparticles called nanodiscs using solution Nuclear Magnetic Resonance spectroscopy.
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spelling Expression and purification of proteins involved in the mitochondrial electron transport chainBiophysical characterization of a novel membrane mimicking systemNADH: ubiquinone oxidoredutaseNanodiscscircular dichroismdynamic light scatteringBiological SciencesBiochemistry for HealthNADH: ubiquinone oxidoredutase is one of the most intricate enzymes. It is part of the respiratory chains and is present in several organisms. This enzyme couples the transfer of two electron, from NADH to ubiquinone, and translocate ions across the membrane establishing a transmembrane difference of electrochemical potential. In this work, we present the initial experimental steps to express and purify a subunit from this intricate enzyme, which is directly related with the translocation of ions (i.e. NuoL). The objective was to characterize the subunit reconstituted in membrane-mimicking nanoparticles called nanodiscs using solution Nuclear Magnetic Resonance spectroscopy.Pereira, ManuelaDuarte, AfonsoRUNSilva, José Pedro Malanho da2018-08-07T16:57:19Z2015-12-0220152015-12-02T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfhttp://hdl.handle.net/10362/43590TID:02247546enginfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T04:23:40Zoai:run.unl.pt:10362/43590Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:31:44.132444Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Expression and purification of proteins involved in the mitochondrial electron transport chain
Biophysical characterization of a novel membrane mimicking system
title Expression and purification of proteins involved in the mitochondrial electron transport chain
spellingShingle Expression and purification of proteins involved in the mitochondrial electron transport chain
Silva, José Pedro Malanho da
NADH: ubiquinone oxidoredutase
Nanodiscs
circular dichroism
dynamic light scattering
Biological Sciences
Biochemistry for Health
title_short Expression and purification of proteins involved in the mitochondrial electron transport chain
title_full Expression and purification of proteins involved in the mitochondrial electron transport chain
title_fullStr Expression and purification of proteins involved in the mitochondrial electron transport chain
title_full_unstemmed Expression and purification of proteins involved in the mitochondrial electron transport chain
title_sort Expression and purification of proteins involved in the mitochondrial electron transport chain
author Silva, José Pedro Malanho da
author_facet Silva, José Pedro Malanho da
author_role author
dc.contributor.none.fl_str_mv Pereira, Manuela
Duarte, Afonso
RUN
dc.contributor.author.fl_str_mv Silva, José Pedro Malanho da
dc.subject.por.fl_str_mv NADH: ubiquinone oxidoredutase
Nanodiscs
circular dichroism
dynamic light scattering
Biological Sciences
Biochemistry for Health
topic NADH: ubiquinone oxidoredutase
Nanodiscs
circular dichroism
dynamic light scattering
Biological Sciences
Biochemistry for Health
description NADH: ubiquinone oxidoredutase is one of the most intricate enzymes. It is part of the respiratory chains and is present in several organisms. This enzyme couples the transfer of two electron, from NADH to ubiquinone, and translocate ions across the membrane establishing a transmembrane difference of electrochemical potential. In this work, we present the initial experimental steps to express and purify a subunit from this intricate enzyme, which is directly related with the translocation of ions (i.e. NuoL). The objective was to characterize the subunit reconstituted in membrane-mimicking nanoparticles called nanodiscs using solution Nuclear Magnetic Resonance spectroscopy.
publishDate 2015
dc.date.none.fl_str_mv 2015-12-02
2015
2015-12-02T00:00:00Z
2018-08-07T16:57:19Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/43590
TID:02247546
url http://hdl.handle.net/10362/43590
identifier_str_mv TID:02247546
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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