Optimization of an Epifluorescence Microscopy Technique for rapid quantification of bacteria asociated to corrosion products in produced water

Detalhes bibliográficos
Autor(a) principal: Silva,D.
Data de Publicação: 2013
Outros Autores: Gonçalves,M., Costa,A.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://scielo.pt/scielo.php?script=sci_arttext&pid=S0870-11642013000400002
Resumo: Microbial cells constitute a severe problem for the petroleum industry. Among the microorganisms, the general anaerobic bacteria (GAnB) are the most common, which includes the sulfate-reducing bacteria (SRB). Nowadays, the GAnB are enumerated by the most probable number (MPN), providing the final quantification after 28 days. In this work, a method for the rapid quantification of bacteria in the produced water from oil extraction was optimized using epifluorescence microscopy with acridine orange dye. This method provided a more accurate estimate of the number of GAnB when compared with enumeration by the MPN technique. Furthermore, it is performed in just 30 minutes per sample.
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spelling Optimization of an Epifluorescence Microscopy Technique for rapid quantification of bacteria asociated to corrosion products in produced waterFluorescence MicroscopyAcridine OrangeAnaerobic BacteriaMicrobial QuantificationProduced Water from OilMicrobial cells constitute a severe problem for the petroleum industry. Among the microorganisms, the general anaerobic bacteria (GAnB) are the most common, which includes the sulfate-reducing bacteria (SRB). Nowadays, the GAnB are enumerated by the most probable number (MPN), providing the final quantification after 28 days. In this work, a method for the rapid quantification of bacteria in the produced water from oil extraction was optimized using epifluorescence microscopy with acridine orange dye. This method provided a more accurate estimate of the number of GAnB when compared with enumeration by the MPN technique. Furthermore, it is performed in just 30 minutes per sample.LNEG - Laboratório Nacional de Energia e Geologia, I.P.2013-12-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articletext/htmlhttp://scielo.pt/scielo.php?script=sci_arttext&pid=S0870-11642013000400002Corrosão e Protecção de Materiais v.32 n.4 2013reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAPenghttp://scielo.pt/scielo.php?script=sci_arttext&pid=S0870-11642013000400002Silva,D.Gonçalves,M.Costa,A.info:eu-repo/semantics/openAccess2024-02-06T16:58:54Zoai:scielo:S0870-11642013000400002Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T02:15:25.198114Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Optimization of an Epifluorescence Microscopy Technique for rapid quantification of bacteria asociated to corrosion products in produced water
title Optimization of an Epifluorescence Microscopy Technique for rapid quantification of bacteria asociated to corrosion products in produced water
spellingShingle Optimization of an Epifluorescence Microscopy Technique for rapid quantification of bacteria asociated to corrosion products in produced water
Silva,D.
Fluorescence Microscopy
Acridine Orange
Anaerobic Bacteria
Microbial Quantification
Produced Water from Oil
title_short Optimization of an Epifluorescence Microscopy Technique for rapid quantification of bacteria asociated to corrosion products in produced water
title_full Optimization of an Epifluorescence Microscopy Technique for rapid quantification of bacteria asociated to corrosion products in produced water
title_fullStr Optimization of an Epifluorescence Microscopy Technique for rapid quantification of bacteria asociated to corrosion products in produced water
title_full_unstemmed Optimization of an Epifluorescence Microscopy Technique for rapid quantification of bacteria asociated to corrosion products in produced water
title_sort Optimization of an Epifluorescence Microscopy Technique for rapid quantification of bacteria asociated to corrosion products in produced water
author Silva,D.
author_facet Silva,D.
Gonçalves,M.
Costa,A.
author_role author
author2 Gonçalves,M.
Costa,A.
author2_role author
author
dc.contributor.author.fl_str_mv Silva,D.
Gonçalves,M.
Costa,A.
dc.subject.por.fl_str_mv Fluorescence Microscopy
Acridine Orange
Anaerobic Bacteria
Microbial Quantification
Produced Water from Oil
topic Fluorescence Microscopy
Acridine Orange
Anaerobic Bacteria
Microbial Quantification
Produced Water from Oil
description Microbial cells constitute a severe problem for the petroleum industry. Among the microorganisms, the general anaerobic bacteria (GAnB) are the most common, which includes the sulfate-reducing bacteria (SRB). Nowadays, the GAnB are enumerated by the most probable number (MPN), providing the final quantification after 28 days. In this work, a method for the rapid quantification of bacteria in the produced water from oil extraction was optimized using epifluorescence microscopy with acridine orange dye. This method provided a more accurate estimate of the number of GAnB when compared with enumeration by the MPN technique. Furthermore, it is performed in just 30 minutes per sample.
publishDate 2013
dc.date.none.fl_str_mv 2013-12-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://scielo.pt/scielo.php?script=sci_arttext&pid=S0870-11642013000400002
url http://scielo.pt/scielo.php?script=sci_arttext&pid=S0870-11642013000400002
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv http://scielo.pt/scielo.php?script=sci_arttext&pid=S0870-11642013000400002
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv LNEG - Laboratório Nacional de Energia e Geologia, I.P.
publisher.none.fl_str_mv LNEG - Laboratório Nacional de Energia e Geologia, I.P.
dc.source.none.fl_str_mv Corrosão e Protecção de Materiais v.32 n.4 2013
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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