Production of catalase-peroxidase and continuous degradation of hydrogen peroxide by an immobilised alkalothermophilic bacillus sp.
Autor(a) principal: | |
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Data de Publicação: | 2003 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/2640 |
Resumo: | Catalase-peroxidase (CP) production by a Bacillus sp. (Bacillus KF) newly isolated from a textile finishing effluent and by this strain immobilised on light expanded clay was studied. In cultivations of Bacillus KF increased catalase activity (about 30-fold) was measured after the addition of H2O2 and Orange IV, while ascorbic acid, pyrogallol and Paraquat, seemed to be poor inducers. Catalases in the cell extract from Bacillus KF showed remarkable stability at high temperatures and pH values with half-lifes of 20 h at pH=9 and 60 °C while half-lifes based on the activity of catalases of only 2.2 h were measured in a column reactor during hydrogen peroxide degradation for the whole cells. However, after the addition of cultivation medium immobilised cells can be regenerated and thus used for textile bleaching effluent treatment. |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Production of catalase-peroxidase and continuous degradation of hydrogen peroxide by an immobilised alkalothermophilic bacillus sp.Catalase-peroxidaseBeachingBacillus sp.bleachingScience & TechnologyCatalase-peroxidase (CP) production by a Bacillus sp. (Bacillus KF) newly isolated from a textile finishing effluent and by this strain immobilised on light expanded clay was studied. In cultivations of Bacillus KF increased catalase activity (about 30-fold) was measured after the addition of H2O2 and Orange IV, while ascorbic acid, pyrogallol and Paraquat, seemed to be poor inducers. Catalases in the cell extract from Bacillus KF showed remarkable stability at high temperatures and pH values with half-lifes of 20 h at pH=9 and 60 °C while half-lifes based on the activity of catalases of only 2.2 h were measured in a column reactor during hydrogen peroxide degradation for the whole cells. However, after the addition of cultivation medium immobilised cells can be regenerated and thus used for textile bleaching effluent treatment.Faculty of Food Technology and BiotechnologyUniversidade do MinhoPaar, AndreasRaninger, AlexanderSousa, FernandaBeurer, IvoPaulo, Artur Cavaco20032003-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/2640eng"Food Technol. Biotechnol". ISSN 1330-9862. 41:2 (2003) 101–104.1330-9862info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:10:09Zoai:repositorium.sdum.uminho.pt:1822/2640Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:01:43.609873Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Production of catalase-peroxidase and continuous degradation of hydrogen peroxide by an immobilised alkalothermophilic bacillus sp. |
title |
Production of catalase-peroxidase and continuous degradation of hydrogen peroxide by an immobilised alkalothermophilic bacillus sp. |
spellingShingle |
Production of catalase-peroxidase and continuous degradation of hydrogen peroxide by an immobilised alkalothermophilic bacillus sp. Paar, Andreas Catalase-peroxidase Beaching Bacillus sp. bleaching Science & Technology |
title_short |
Production of catalase-peroxidase and continuous degradation of hydrogen peroxide by an immobilised alkalothermophilic bacillus sp. |
title_full |
Production of catalase-peroxidase and continuous degradation of hydrogen peroxide by an immobilised alkalothermophilic bacillus sp. |
title_fullStr |
Production of catalase-peroxidase and continuous degradation of hydrogen peroxide by an immobilised alkalothermophilic bacillus sp. |
title_full_unstemmed |
Production of catalase-peroxidase and continuous degradation of hydrogen peroxide by an immobilised alkalothermophilic bacillus sp. |
title_sort |
Production of catalase-peroxidase and continuous degradation of hydrogen peroxide by an immobilised alkalothermophilic bacillus sp. |
author |
Paar, Andreas |
author_facet |
Paar, Andreas Raninger, Alexander Sousa, Fernanda Beurer, Ivo Paulo, Artur Cavaco |
author_role |
author |
author2 |
Raninger, Alexander Sousa, Fernanda Beurer, Ivo Paulo, Artur Cavaco |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Paar, Andreas Raninger, Alexander Sousa, Fernanda Beurer, Ivo Paulo, Artur Cavaco |
dc.subject.por.fl_str_mv |
Catalase-peroxidase Beaching Bacillus sp. bleaching Science & Technology |
topic |
Catalase-peroxidase Beaching Bacillus sp. bleaching Science & Technology |
description |
Catalase-peroxidase (CP) production by a Bacillus sp. (Bacillus KF) newly isolated from a textile finishing effluent and by this strain immobilised on light expanded clay was studied. In cultivations of Bacillus KF increased catalase activity (about 30-fold) was measured after the addition of H2O2 and Orange IV, while ascorbic acid, pyrogallol and Paraquat, seemed to be poor inducers. Catalases in the cell extract from Bacillus KF showed remarkable stability at high temperatures and pH values with half-lifes of 20 h at pH=9 and 60 °C while half-lifes based on the activity of catalases of only 2.2 h were measured in a column reactor during hydrogen peroxide degradation for the whole cells. However, after the addition of cultivation medium immobilised cells can be regenerated and thus used for textile bleaching effluent treatment. |
publishDate |
2003 |
dc.date.none.fl_str_mv |
2003 2003-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/2640 |
url |
http://hdl.handle.net/1822/2640 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
"Food Technol. Biotechnol". ISSN 1330-9862. 41:2 (2003) 101–104. 1330-9862 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Faculty of Food Technology and Biotechnology |
publisher.none.fl_str_mv |
Faculty of Food Technology and Biotechnology |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1799132416806223872 |