Production of catalase-peroxidase and continuous degradation of hydrogen peroxide by an immobilised alkalothermophilic bacillus sp.

Detalhes bibliográficos
Autor(a) principal: Paar, Andreas
Data de Publicação: 2003
Outros Autores: Raninger, Alexander, Sousa, Fernanda, Beurer, Ivo, Paulo, Artur Cavaco
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/2640
Resumo: Catalase-peroxidase (CP) production by a Bacillus sp. (Bacillus KF) newly isolated from a textile finishing effluent and by this strain immobilised on light expanded clay was studied. In cultivations of Bacillus KF increased catalase activity (about 30-fold) was measured after the addition of H2O2 and Orange IV, while ascorbic acid, pyrogallol and Paraquat, seemed to be poor inducers. Catalases in the cell extract from Bacillus KF showed remarkable stability at high temperatures and pH values with half-lifes of 20 h at pH=9 and 60 °C while half-lifes based on the activity of catalases of only 2.2 h were measured in a column reactor during hydrogen peroxide degradation for the whole cells. However, after the addition of cultivation medium immobilised cells can be regenerated and thus used for textile bleaching effluent treatment.
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spelling Production of catalase-peroxidase and continuous degradation of hydrogen peroxide by an immobilised alkalothermophilic bacillus sp.Catalase-peroxidaseBeachingBacillus sp.bleachingScience & TechnologyCatalase-peroxidase (CP) production by a Bacillus sp. (Bacillus KF) newly isolated from a textile finishing effluent and by this strain immobilised on light expanded clay was studied. In cultivations of Bacillus KF increased catalase activity (about 30-fold) was measured after the addition of H2O2 and Orange IV, while ascorbic acid, pyrogallol and Paraquat, seemed to be poor inducers. Catalases in the cell extract from Bacillus KF showed remarkable stability at high temperatures and pH values with half-lifes of 20 h at pH=9 and 60 °C while half-lifes based on the activity of catalases of only 2.2 h were measured in a column reactor during hydrogen peroxide degradation for the whole cells. However, after the addition of cultivation medium immobilised cells can be regenerated and thus used for textile bleaching effluent treatment.Faculty of Food Technology and BiotechnologyUniversidade do MinhoPaar, AndreasRaninger, AlexanderSousa, FernandaBeurer, IvoPaulo, Artur Cavaco20032003-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/2640eng"Food Technol. Biotechnol". ISSN 1330-9862. 41:2 (2003) 101–104.1330-9862info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:10:09Zoai:repositorium.sdum.uminho.pt:1822/2640Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:01:43.609873Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Production of catalase-peroxidase and continuous degradation of hydrogen peroxide by an immobilised alkalothermophilic bacillus sp.
title Production of catalase-peroxidase and continuous degradation of hydrogen peroxide by an immobilised alkalothermophilic bacillus sp.
spellingShingle Production of catalase-peroxidase and continuous degradation of hydrogen peroxide by an immobilised alkalothermophilic bacillus sp.
Paar, Andreas
Catalase-peroxidase
Beaching
Bacillus sp.
bleaching
Science & Technology
title_short Production of catalase-peroxidase and continuous degradation of hydrogen peroxide by an immobilised alkalothermophilic bacillus sp.
title_full Production of catalase-peroxidase and continuous degradation of hydrogen peroxide by an immobilised alkalothermophilic bacillus sp.
title_fullStr Production of catalase-peroxidase and continuous degradation of hydrogen peroxide by an immobilised alkalothermophilic bacillus sp.
title_full_unstemmed Production of catalase-peroxidase and continuous degradation of hydrogen peroxide by an immobilised alkalothermophilic bacillus sp.
title_sort Production of catalase-peroxidase and continuous degradation of hydrogen peroxide by an immobilised alkalothermophilic bacillus sp.
author Paar, Andreas
author_facet Paar, Andreas
Raninger, Alexander
Sousa, Fernanda
Beurer, Ivo
Paulo, Artur Cavaco
author_role author
author2 Raninger, Alexander
Sousa, Fernanda
Beurer, Ivo
Paulo, Artur Cavaco
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Paar, Andreas
Raninger, Alexander
Sousa, Fernanda
Beurer, Ivo
Paulo, Artur Cavaco
dc.subject.por.fl_str_mv Catalase-peroxidase
Beaching
Bacillus sp.
bleaching
Science & Technology
topic Catalase-peroxidase
Beaching
Bacillus sp.
bleaching
Science & Technology
description Catalase-peroxidase (CP) production by a Bacillus sp. (Bacillus KF) newly isolated from a textile finishing effluent and by this strain immobilised on light expanded clay was studied. In cultivations of Bacillus KF increased catalase activity (about 30-fold) was measured after the addition of H2O2 and Orange IV, while ascorbic acid, pyrogallol and Paraquat, seemed to be poor inducers. Catalases in the cell extract from Bacillus KF showed remarkable stability at high temperatures and pH values with half-lifes of 20 h at pH=9 and 60 °C while half-lifes based on the activity of catalases of only 2.2 h were measured in a column reactor during hydrogen peroxide degradation for the whole cells. However, after the addition of cultivation medium immobilised cells can be regenerated and thus used for textile bleaching effluent treatment.
publishDate 2003
dc.date.none.fl_str_mv 2003
2003-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/2640
url http://hdl.handle.net/1822/2640
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv "Food Technol. Biotechnol". ISSN 1330-9862. 41:2 (2003) 101–104.
1330-9862
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Faculty of Food Technology and Biotechnology
publisher.none.fl_str_mv Faculty of Food Technology and Biotechnology
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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