Characterization of the canine CD20 as a therapeutic target for comparative passive immunotherapy

Detalhes bibliográficos
Autor(a) principal: Dias, Joana N. R.
Data de Publicação: 2022
Outros Autores: Almeida, André, S. André, Ana, Aguiar, Sandra I, Bule, Pedro, Nogueira, Sara, Oliveira, Soraia S, Carrapiço, Belmira, Gil, Solange, Tavares, Luis, Aires Da Silva, Frederico
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.5/24153
Resumo: Research Areas: Science & Technology
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spelling Characterization of the canine CD20 as a therapeutic target for comparative passive immunotherapyMonoclonal-Antibody TherapyB-CellAnimal-ModelRituximaBlymphomaExpressionGeneRepertoireCancerIdentificationResearch Areas: Science & TechnologyAnti-CD20 therapies have revolutionized the treatment of B-cell malignancies. Despite these advances, relapsed and refractory disease remains a major treatment challenge. The optimization of CD20-targeted immunotherapies is considered a promising strategy to improve current therapies. However, research has been limited by the scarcity of preclinical models that recapitulate the complex interaction between the immune system and cancers. The addition of the canine lymphoma (cNHL) model in the development of anti-CD20 therapies may provide a clinically relevant approach for the translation of improved immunotherapies. Still, an anti-CD20 therapy for cNHL has not been established stressing the need of a comprehensive target characterization. Herein, we performed an in-depth characterization on canine CD20 mRNA transcript and protein expression in a cNHL biobank and demonstrated a canine CD20 overexpression in B-cell lymphoma samples. Moreover, CD20 gene sequencing analysis identifed six amino acid diferences in patient samples (C77Y, L147F, I159M, L198V, A201T and G273E). Finally, we reported the use of a novel strategy for the generation of anti-CD20 mAbs, with human and canine cross-reactivity, by exploring our rabbit derived singledomain antibody platform. Overall, these results support the rationale of using CD20 as a target for veterinary settings and the development of novel therapeutics and immunodiagnostics.Nature ResearchRepositório da Universidade de LisboaDias, Joana N. R.Almeida, AndréS. André, AnaAguiar, Sandra IBule, PedroNogueira, SaraOliveira, Soraia SCarrapiço, BelmiraGil, SolangeTavares, LuisAires Da Silva, Frederico2022-04-24T22:16:11Z2022-02-172022-02-17T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.5/24153engDias JNR, Almeida A, Andre AS, Aguiar SI, Bule P, Nogueira S, Oliveira SS, Carrapico B, Gil S, Tavares L, Aires-da-Silva F. 2022. Characterization of the canine CD20 as a therapeutic target for comparative passive immunotherapy. Scientific Reports, 12(1):2678. Doi 10.1038/s41598-022-06549-12045-232210.1038/s41598-022-06549-1info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-03-06T14:53:48Zoai:www.repository.utl.pt:10400.5/24153Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T17:08:16.026451Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Characterization of the canine CD20 as a therapeutic target for comparative passive immunotherapy
title Characterization of the canine CD20 as a therapeutic target for comparative passive immunotherapy
spellingShingle Characterization of the canine CD20 as a therapeutic target for comparative passive immunotherapy
Dias, Joana N. R.
Monoclonal-Antibody Therapy
B-Cell
Animal-Model
Rituxima
Blymphoma
Expression
Gene
Repertoire
Cancer
Identification
title_short Characterization of the canine CD20 as a therapeutic target for comparative passive immunotherapy
title_full Characterization of the canine CD20 as a therapeutic target for comparative passive immunotherapy
title_fullStr Characterization of the canine CD20 as a therapeutic target for comparative passive immunotherapy
title_full_unstemmed Characterization of the canine CD20 as a therapeutic target for comparative passive immunotherapy
title_sort Characterization of the canine CD20 as a therapeutic target for comparative passive immunotherapy
author Dias, Joana N. R.
author_facet Dias, Joana N. R.
Almeida, André
S. André, Ana
Aguiar, Sandra I
Bule, Pedro
Nogueira, Sara
Oliveira, Soraia S
Carrapiço, Belmira
Gil, Solange
Tavares, Luis
Aires Da Silva, Frederico
author_role author
author2 Almeida, André
S. André, Ana
Aguiar, Sandra I
Bule, Pedro
Nogueira, Sara
Oliveira, Soraia S
Carrapiço, Belmira
Gil, Solange
Tavares, Luis
Aires Da Silva, Frederico
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Dias, Joana N. R.
Almeida, André
S. André, Ana
Aguiar, Sandra I
Bule, Pedro
Nogueira, Sara
Oliveira, Soraia S
Carrapiço, Belmira
Gil, Solange
Tavares, Luis
Aires Da Silva, Frederico
dc.subject.por.fl_str_mv Monoclonal-Antibody Therapy
B-Cell
Animal-Model
Rituxima
Blymphoma
Expression
Gene
Repertoire
Cancer
Identification
topic Monoclonal-Antibody Therapy
B-Cell
Animal-Model
Rituxima
Blymphoma
Expression
Gene
Repertoire
Cancer
Identification
description Research Areas: Science & Technology
publishDate 2022
dc.date.none.fl_str_mv 2022-04-24T22:16:11Z
2022-02-17
2022-02-17T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.5/24153
url http://hdl.handle.net/10400.5/24153
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Dias JNR, Almeida A, Andre AS, Aguiar SI, Bule P, Nogueira S, Oliveira SS, Carrapico B, Gil S, Tavares L, Aires-da-Silva F. 2022. Characterization of the canine CD20 as a therapeutic target for comparative passive immunotherapy. Scientific Reports, 12(1):2678. Doi 10.1038/s41598-022-06549-1
2045-2322
10.1038/s41598-022-06549-1
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Nature Research
publisher.none.fl_str_mv Nature Research
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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