Comparative effects of sediments contaminated by carcinogenic and non-carcinogenic PAHs in Dicentrarchus labrax: a semi-quantitative histopathological approach

Detalhes bibliográficos
Autor(a) principal: Santos, José Miguel de Oliveira Dias Prudente dos
Data de Publicação: 2013
Tipo de documento: Dissertação
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/10771
Resumo: Dissertação para obtenção do Grau de Mestre em Engenharia do Ambiente
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spelling Comparative effects of sediments contaminated by carcinogenic and non-carcinogenic PAHs in Dicentrarchus labrax: a semi-quantitative histopathological approachPolycyclic aromatic hydrocarbonsSeabassesHistologyLiverCarcinogenicNon-carcinogenicDissertação para obtenção do Grau de Mestre em Engenharia do AmbientePolycyclic Aromatic Hydrocarbons (PAHs) are considered priority pollutants due to their high risk to environmental and human health. Due to their hydrophobic character, in aquatic environments, these substances tend to adsorb to the particulate fraction and accumulate in the sediments. Despite their division into carcinogenic, potentially carcinogenic and non-carcinogenic to humans, little is known about the differences between modes of action of carcinogenic and non-carcinogenic PAHs in aquatic organisms. In order to understand the toxicity mechanisms of these two classes, laboratory assays were performed with juvenile basses (Dicentrarchus labrax) exposed to contaminated artificial sediments for 28 days. Sediment were spiked with environmentally-relevant concentrations of benzo[b]fluoranthene (a carcinogenic PAH) and phenanthrene (non-carcinogenic), either isolated or in mixture. Exposure effects were analysed through an indice-based semi-quantitative histopathological approach in hepatic tissue, due to the role of liver in the accumulation and detoxification of xenobiotics. Overall, significant alterations in the hepatic tissue were detected relatively to control tests, either for isolated or mixture assays, despite the low levels of exposure. Individuals exposed to benzo[b]fluoranthene presented higher severity and number of hepatic lesions compared to phenanthrene. Furthermore different toxicants caused different patterns of histopathological lesions and alterations. The results also show that histopathological condition indices of mixture-exposed individuals do not match the expected additive effects, suggesting a possible synergistic interaction effect between the contaminants. This work allows the conclusion that, albeit considered low, environmentally-relevant concentrations of PAHs in sediments may cause adverse effects in organisms, in this case, a demersal fish. On the other hand, results also suggest that a non-carcinogenic PAH may be responsible for considerable toxic effects, even in moderate concentrations. Altogether, requalifying risk assessment for these substances becomes of the upmost importance since PAHs (as other pollutants) are usually present in the environment in complex mixtures.Faculdade de Ciências e TecnologiaCosta, Maria HelenaRUNSantos, José Miguel de Oliveira Dias Prudente dos2013-11-26T17:18:20Z20132013-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfhttp://hdl.handle.net/10362/10771enginfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T03:44:42Zoai:run.unl.pt:10362/10771Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:19:43.000866Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Comparative effects of sediments contaminated by carcinogenic and non-carcinogenic PAHs in Dicentrarchus labrax: a semi-quantitative histopathological approach
title Comparative effects of sediments contaminated by carcinogenic and non-carcinogenic PAHs in Dicentrarchus labrax: a semi-quantitative histopathological approach
spellingShingle Comparative effects of sediments contaminated by carcinogenic and non-carcinogenic PAHs in Dicentrarchus labrax: a semi-quantitative histopathological approach
Santos, José Miguel de Oliveira Dias Prudente dos
Polycyclic aromatic hydrocarbons
Seabasses
Histology
Liver
Carcinogenic
Non-carcinogenic
title_short Comparative effects of sediments contaminated by carcinogenic and non-carcinogenic PAHs in Dicentrarchus labrax: a semi-quantitative histopathological approach
title_full Comparative effects of sediments contaminated by carcinogenic and non-carcinogenic PAHs in Dicentrarchus labrax: a semi-quantitative histopathological approach
title_fullStr Comparative effects of sediments contaminated by carcinogenic and non-carcinogenic PAHs in Dicentrarchus labrax: a semi-quantitative histopathological approach
title_full_unstemmed Comparative effects of sediments contaminated by carcinogenic and non-carcinogenic PAHs in Dicentrarchus labrax: a semi-quantitative histopathological approach
title_sort Comparative effects of sediments contaminated by carcinogenic and non-carcinogenic PAHs in Dicentrarchus labrax: a semi-quantitative histopathological approach
author Santos, José Miguel de Oliveira Dias Prudente dos
author_facet Santos, José Miguel de Oliveira Dias Prudente dos
author_role author
dc.contributor.none.fl_str_mv Costa, Maria Helena
RUN
dc.contributor.author.fl_str_mv Santos, José Miguel de Oliveira Dias Prudente dos
dc.subject.por.fl_str_mv Polycyclic aromatic hydrocarbons
Seabasses
Histology
Liver
Carcinogenic
Non-carcinogenic
topic Polycyclic aromatic hydrocarbons
Seabasses
Histology
Liver
Carcinogenic
Non-carcinogenic
description Dissertação para obtenção do Grau de Mestre em Engenharia do Ambiente
publishDate 2013
dc.date.none.fl_str_mv 2013-11-26T17:18:20Z
2013
2013-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/10771
url http://hdl.handle.net/10362/10771
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Faculdade de Ciências e Tecnologia
publisher.none.fl_str_mv Faculdade de Ciências e Tecnologia
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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