Mycobiota in Chilean chilli Capsicum annuum L. used for production of Merkén
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/66978 |
Resumo: | This work aims to provide the first study on the mycobiota present in Chilean pepper Capsicum annuum L. cv. Cacho de Cabra throughout the early production stages. Two hundred and forty berry fruits were sampled: 1) at the ripe fruits harvest day; 2) during drying; and 3) smoking processes. A total of 192 strains, encompassing 11 genera and 44 species, were identified through analysis of -tubulin (benA) gene and internal transcribed spacer of ribosomal DNA (ITS) region. All collection points showed samples with high fungal contamination, but the mycobiota composition varied as a result of different environmental conditions. Alternaria spp. and Fusarium spp. were predominantly isolated from fresh fruits of C. annuum. Penicillium spp. was the most frequent genus in all analysed points. Penicillium brevicompactum and P. crustosum were the most abundant species. Among Aspergillus, A. niger and A. flavus were dominant after the drying phase. In our study, none of the analysed strains of Penicillium (113) and Aspergillus (35) produced Ochratoxin A at detectable levels. The broad characterization of the fungal community of C. annuum carried out in this study, could be a guideline for future mycotoxin analyses performed directly on the pod. Understanding the role and dynamics of mycobiota and its relationship with the toxins present in this substrate, will be useful to establish and improve control measures considering the specificities of each point in the C. annuum production chain. |
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Mycobiota in Chilean chilli Capsicum annuum L. used for production of MerkénChilliSpoilage FungiMycotoxigenic FungiMycobiota IdentificationOTACiências Agrárias::Biotecnologia Agrária e AlimentarScience & TechnologyThis work aims to provide the first study on the mycobiota present in Chilean pepper Capsicum annuum L. cv. Cacho de Cabra throughout the early production stages. Two hundred and forty berry fruits were sampled: 1) at the ripe fruits harvest day; 2) during drying; and 3) smoking processes. A total of 192 strains, encompassing 11 genera and 44 species, were identified through analysis of -tubulin (benA) gene and internal transcribed spacer of ribosomal DNA (ITS) region. All collection points showed samples with high fungal contamination, but the mycobiota composition varied as a result of different environmental conditions. Alternaria spp. and Fusarium spp. were predominantly isolated from fresh fruits of C. annuum. Penicillium spp. was the most frequent genus in all analysed points. Penicillium brevicompactum and P. crustosum were the most abundant species. Among Aspergillus, A. niger and A. flavus were dominant after the drying phase. In our study, none of the analysed strains of Penicillium (113) and Aspergillus (35) produced Ochratoxin A at detectable levels. The broad characterization of the fungal community of C. annuum carried out in this study, could be a guideline for future mycotoxin analyses performed directly on the pod. Understanding the role and dynamics of mycobiota and its relationship with the toxins present in this substrate, will be useful to establish and improve control measures considering the specificities of each point in the C. annuum production chain.CONICYT/Chile for her Ph.D. grant no 21181445. R.R thanks to CONICYT/Chile for his MSc. grant Folio N° 7317076, Application N° 73170764. The present work was partially funded by the Universidad de La Frontera (Temuco, Chile) through the Project DIUFRO PIA19-0001. Moreover, this study was also supported by the Portuguese Foundation for Science and Technology (FCT) under the scope of the strategic funding of UID/BIO/04469/2019 unit and BioTecNorte operation (NORTE-01-0145-FEDER-000004) funded by the European Regional Development Fund under the scope of Norte2020 - Programa Operacional Regional do Norte.info:eu-repo/semantics/publishedVersionElsevierUniversidade do MinhoCosta, JéssicaRodriguez, R.Santos, Carla Isabel ArcanjoSoares, CéliaLima, NelsonSantos, Cledir2020-12-022020-12-02T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/66978engCosta, Jéssica; Rodriguez, R.; Santos, Carla; Soares, Célia; Lima, Nelson; Santos, Cledir, Mycobiota in Chilean chilli Capsicum annuum L. used for production of Merkén. International Journal of Food Microbiology, 334(108833), 20200168-160510.1016/j.ijfoodmicro.2020.10883332911159https://www.sciencedirect.com/science/article/pii/S0168160520303275info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:05:09Zoai:repositorium.sdum.uminho.pt:1822/66978Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:55:32.840193Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Mycobiota in Chilean chilli Capsicum annuum L. used for production of Merkén |
title |
Mycobiota in Chilean chilli Capsicum annuum L. used for production of Merkén |
spellingShingle |
Mycobiota in Chilean chilli Capsicum annuum L. used for production of Merkén Costa, Jéssica Chilli Spoilage Fungi Mycotoxigenic Fungi Mycobiota Identification OTA Ciências Agrárias::Biotecnologia Agrária e Alimentar Science & Technology |
title_short |
Mycobiota in Chilean chilli Capsicum annuum L. used for production of Merkén |
title_full |
Mycobiota in Chilean chilli Capsicum annuum L. used for production of Merkén |
title_fullStr |
Mycobiota in Chilean chilli Capsicum annuum L. used for production of Merkén |
title_full_unstemmed |
Mycobiota in Chilean chilli Capsicum annuum L. used for production of Merkén |
title_sort |
Mycobiota in Chilean chilli Capsicum annuum L. used for production of Merkén |
author |
Costa, Jéssica |
author_facet |
Costa, Jéssica Rodriguez, R. Santos, Carla Isabel Arcanjo Soares, Célia Lima, Nelson Santos, Cledir |
author_role |
author |
author2 |
Rodriguez, R. Santos, Carla Isabel Arcanjo Soares, Célia Lima, Nelson Santos, Cledir |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Costa, Jéssica Rodriguez, R. Santos, Carla Isabel Arcanjo Soares, Célia Lima, Nelson Santos, Cledir |
dc.subject.por.fl_str_mv |
Chilli Spoilage Fungi Mycotoxigenic Fungi Mycobiota Identification OTA Ciências Agrárias::Biotecnologia Agrária e Alimentar Science & Technology |
topic |
Chilli Spoilage Fungi Mycotoxigenic Fungi Mycobiota Identification OTA Ciências Agrárias::Biotecnologia Agrária e Alimentar Science & Technology |
description |
This work aims to provide the first study on the mycobiota present in Chilean pepper Capsicum annuum L. cv. Cacho de Cabra throughout the early production stages. Two hundred and forty berry fruits were sampled: 1) at the ripe fruits harvest day; 2) during drying; and 3) smoking processes. A total of 192 strains, encompassing 11 genera and 44 species, were identified through analysis of -tubulin (benA) gene and internal transcribed spacer of ribosomal DNA (ITS) region. All collection points showed samples with high fungal contamination, but the mycobiota composition varied as a result of different environmental conditions. Alternaria spp. and Fusarium spp. were predominantly isolated from fresh fruits of C. annuum. Penicillium spp. was the most frequent genus in all analysed points. Penicillium brevicompactum and P. crustosum were the most abundant species. Among Aspergillus, A. niger and A. flavus were dominant after the drying phase. In our study, none of the analysed strains of Penicillium (113) and Aspergillus (35) produced Ochratoxin A at detectable levels. The broad characterization of the fungal community of C. annuum carried out in this study, could be a guideline for future mycotoxin analyses performed directly on the pod. Understanding the role and dynamics of mycobiota and its relationship with the toxins present in this substrate, will be useful to establish and improve control measures considering the specificities of each point in the C. annuum production chain. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-12-02 2020-12-02T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/66978 |
url |
http://hdl.handle.net/1822/66978 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Costa, Jéssica; Rodriguez, R.; Santos, Carla; Soares, Célia; Lima, Nelson; Santos, Cledir, Mycobiota in Chilean chilli Capsicum annuum L. used for production of Merkén. International Journal of Food Microbiology, 334(108833), 2020 0168-1605 10.1016/j.ijfoodmicro.2020.108833 32911159 https://www.sciencedirect.com/science/article/pii/S0168160520303275 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
dc.source.none.fl_str_mv |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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