Depletion of the FtsH1/3 proteolytic complex suppresses the nutrient stress response in the cyanobacterium synechocystis sp strain PCC 6803
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.1/14150 |
Resumo: | The membrane-embedded FtsH proteases found in bacteria, chloroplasts, and mitochondria are involved in diverse cellular processes including protein quality control and regulation. The genome of the model cyanobacterium Synechocystis sp PCC 6803 encodes four FtsH homologs designated FtsH1 to FtsH4. The FtsH3 homolog is present in two hetero-oligomeric complexes: FtsH2/3, which is responsible for photosystem II quality control, and the essential FtsH1/3 complex, which helps maintain Fe homeostasis by regulating the level of the transcription factor Fur. To gain a more comprehensive insight into the physiological roles of FtsH hetero-complexes, we performed genome-wide expression profiling and global proteomic analyses of Synechocystis mutants conditionally depleted of FtsH3 or FtsH1 grown under various nutrient conditions. We show that the lack of FtsH1/3 leads to a drastic reduction in the transcriptional response to nutrient stress of not only Fur but also the Pho, NdhR, and NtcA regulons. In addition, this effect is accompanied by the accumulation of the respective transcription factors. Thus, the FtsH1/3 complex is of critical importance for acclimation to iron, phosphate, carbon, and nitrogen starvation in Synechocystis. |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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7160 |
spelling |
Depletion of the FtsH1/3 proteolytic complex suppresses the nutrient stress response in the cyanobacterium synechocystis sp strain PCC 6803Signal transductionPhotosystem IiEscherichia coliNitrogen starvationGene expressionProteinPhosphateRegulatorAccumulationAcclimationThe membrane-embedded FtsH proteases found in bacteria, chloroplasts, and mitochondria are involved in diverse cellular processes including protein quality control and regulation. The genome of the model cyanobacterium Synechocystis sp PCC 6803 encodes four FtsH homologs designated FtsH1 to FtsH4. The FtsH3 homolog is present in two hetero-oligomeric complexes: FtsH2/3, which is responsible for photosystem II quality control, and the essential FtsH1/3 complex, which helps maintain Fe homeostasis by regulating the level of the transcription factor Fur. To gain a more comprehensive insight into the physiological roles of FtsH hetero-complexes, we performed genome-wide expression profiling and global proteomic analyses of Synechocystis mutants conditionally depleted of FtsH3 or FtsH1 grown under various nutrient conditions. We show that the lack of FtsH1/3 leads to a drastic reduction in the transcriptional response to nutrient stress of not only Fur but also the Pho, NdhR, and NtcA regulons. In addition, this effect is accompanied by the accumulation of the respective transcription factors. Thus, the FtsH1/3 complex is of critical importance for acclimation to iron, phosphate, carbon, and nitrogen starvation in Synechocystis.Germany Federal Ministry of Education and Research [031L0106B]Grant Agency of the Czech RepublicGrant Agency of the Czech Republic [P501-12-G055]Czech Ministry of Education Ministry of Education, Youth & Sports - Czech Republic [LO1416]Portuguese Fundacao para a Ciencia e a Tecnologia (Foundation for Science and Technology) [PTDC/BIA-MIC/4418/2012, IF/00881/2013, UID/Multi/04326/2013]United Kingdom Biotechnology and Biological Sciences Research Council (BBSRC)Biotechnology and Biological Sciences Research Council (BBSRC) [BB/M012166/1, BB/M000265/1]European Research CouncilEuropean Research Council (ERC) [338895]American Society of Plant BiologistsSapientiaKrynicka, VendulaGeorg, JensJackson, Philip J.Dickman, Mark J.Hunter, C. NeilFutschik, MatthiasHess, Wolfgang R.Komenda, Josef2020-07-24T10:50:46Z2019-122019-12-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.1/14150eng1040-465110.1105/tpc.19.00411info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-24T10:26:24Zoai:sapientia.ualg.pt:10400.1/14150Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T20:05:12.225534Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Depletion of the FtsH1/3 proteolytic complex suppresses the nutrient stress response in the cyanobacterium synechocystis sp strain PCC 6803 |
title |
Depletion of the FtsH1/3 proteolytic complex suppresses the nutrient stress response in the cyanobacterium synechocystis sp strain PCC 6803 |
spellingShingle |
Depletion of the FtsH1/3 proteolytic complex suppresses the nutrient stress response in the cyanobacterium synechocystis sp strain PCC 6803 Krynicka, Vendula Signal transduction Photosystem Ii Escherichia coli Nitrogen starvation Gene expression Protein Phosphate Regulator Accumulation Acclimation |
title_short |
Depletion of the FtsH1/3 proteolytic complex suppresses the nutrient stress response in the cyanobacterium synechocystis sp strain PCC 6803 |
title_full |
Depletion of the FtsH1/3 proteolytic complex suppresses the nutrient stress response in the cyanobacterium synechocystis sp strain PCC 6803 |
title_fullStr |
Depletion of the FtsH1/3 proteolytic complex suppresses the nutrient stress response in the cyanobacterium synechocystis sp strain PCC 6803 |
title_full_unstemmed |
Depletion of the FtsH1/3 proteolytic complex suppresses the nutrient stress response in the cyanobacterium synechocystis sp strain PCC 6803 |
title_sort |
Depletion of the FtsH1/3 proteolytic complex suppresses the nutrient stress response in the cyanobacterium synechocystis sp strain PCC 6803 |
author |
Krynicka, Vendula |
author_facet |
Krynicka, Vendula Georg, Jens Jackson, Philip J. Dickman, Mark J. Hunter, C. Neil Futschik, Matthias Hess, Wolfgang R. Komenda, Josef |
author_role |
author |
author2 |
Georg, Jens Jackson, Philip J. Dickman, Mark J. Hunter, C. Neil Futschik, Matthias Hess, Wolfgang R. Komenda, Josef |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Sapientia |
dc.contributor.author.fl_str_mv |
Krynicka, Vendula Georg, Jens Jackson, Philip J. Dickman, Mark J. Hunter, C. Neil Futschik, Matthias Hess, Wolfgang R. Komenda, Josef |
dc.subject.por.fl_str_mv |
Signal transduction Photosystem Ii Escherichia coli Nitrogen starvation Gene expression Protein Phosphate Regulator Accumulation Acclimation |
topic |
Signal transduction Photosystem Ii Escherichia coli Nitrogen starvation Gene expression Protein Phosphate Regulator Accumulation Acclimation |
description |
The membrane-embedded FtsH proteases found in bacteria, chloroplasts, and mitochondria are involved in diverse cellular processes including protein quality control and regulation. The genome of the model cyanobacterium Synechocystis sp PCC 6803 encodes four FtsH homologs designated FtsH1 to FtsH4. The FtsH3 homolog is present in two hetero-oligomeric complexes: FtsH2/3, which is responsible for photosystem II quality control, and the essential FtsH1/3 complex, which helps maintain Fe homeostasis by regulating the level of the transcription factor Fur. To gain a more comprehensive insight into the physiological roles of FtsH hetero-complexes, we performed genome-wide expression profiling and global proteomic analyses of Synechocystis mutants conditionally depleted of FtsH3 or FtsH1 grown under various nutrient conditions. We show that the lack of FtsH1/3 leads to a drastic reduction in the transcriptional response to nutrient stress of not only Fur but also the Pho, NdhR, and NtcA regulons. In addition, this effect is accompanied by the accumulation of the respective transcription factors. Thus, the FtsH1/3 complex is of critical importance for acclimation to iron, phosphate, carbon, and nitrogen starvation in Synechocystis. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-12 2019-12-01T00:00:00Z 2020-07-24T10:50:46Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.1/14150 |
url |
http://hdl.handle.net/10400.1/14150 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
1040-4651 10.1105/tpc.19.00411 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
American Society of Plant Biologists |
publisher.none.fl_str_mv |
American Society of Plant Biologists |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1799133291347968000 |