ZrFsy1, a high-affinity fructose/H+ symporter from fructophilic Zygosaccharomyces rouxii
Autor(a) principal: | |
---|---|
Data de Publicação: | 2013 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.5/17783 |
Resumo: | Zygosaccharomyces rouxii is a fructophilic yeast than can grow at very high sugar concentrations. We have identified an ORF encoding a putative fructose/H+ symporter in the Z. rouxii CBS 732 genome database. Heterologous expression of this ORF in a S. cerevisiae strain lacking its own hexose transporters (hxt-null) and subsequent kinetic characterization of its sugar transport activity showed it is a high-affinity low-capacity fructose/H+ symporter, with Km 0.4560.07 mM and Vmax 0.5760.02 mmol h21 (gdw) 21. We named it ZrFsy1. This protein also weakly transports xylitol and sorbose, but not glucose or other hexoses. The expression of ZrFSY1 in Z. rouxii is higher when the cells are cultivated at extremely low fructose concentrations (,0.2%) and on non-fermentable carbon sources such as mannitol and xylitol, where the cells have a prolonged lag phase, longer duplication times and change their microscopic morphology. A clear phenotype was determined for the first time for the deletion of a fructose/H+ symporter in the genome where it occurs naturally. The effect of the deletion of ZrFSY1 in Z. rouxii cells is only evident when the cells are cultivated at very low fructose concentrations, when the ZrFsy1 fructose symporter is the main active fructose transporter system |
id |
RCAP_1b98152f0a2ac70b25d1af533a1e7742 |
---|---|
oai_identifier_str |
oai:www.repository.utl.pt:10400.5/17783 |
network_acronym_str |
RCAP |
network_name_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository_id_str |
7160 |
spelling |
ZrFsy1, a high-affinity fructose/H+ symporter from fructophilic Zygosaccharomyces rouxiiZygosaccharomyces rouxiifructoseZygosaccharomyces rouxii is a fructophilic yeast than can grow at very high sugar concentrations. We have identified an ORF encoding a putative fructose/H+ symporter in the Z. rouxii CBS 732 genome database. Heterologous expression of this ORF in a S. cerevisiae strain lacking its own hexose transporters (hxt-null) and subsequent kinetic characterization of its sugar transport activity showed it is a high-affinity low-capacity fructose/H+ symporter, with Km 0.4560.07 mM and Vmax 0.5760.02 mmol h21 (gdw) 21. We named it ZrFsy1. This protein also weakly transports xylitol and sorbose, but not glucose or other hexoses. The expression of ZrFSY1 in Z. rouxii is higher when the cells are cultivated at extremely low fructose concentrations (,0.2%) and on non-fermentable carbon sources such as mannitol and xylitol, where the cells have a prolonged lag phase, longer duplication times and change their microscopic morphology. A clear phenotype was determined for the first time for the deletion of a fructose/H+ symporter in the genome where it occurs naturally. The effect of the deletion of ZrFSY1 in Z. rouxii cells is only evident when the cells are cultivated at very low fructose concentrations, when the ZrFsy1 fructose symporter is the main active fructose transporter systemPlosOneRepositório da Universidade de LisboaLeandro, Maria JoséSychrova, HanaPrista, CatarinaLoureiro-Dias, M.C.2019-05-09T13:32:42Z20132013-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.5/17783engLeandro MJ, Sychrova´ H, Prista C, Loureiro-Dias MC (2013) ZrFsy1, a High-Affinity Fructose/H+ Symporter from Fructophilic Yeast Zygosaccharomyces rouxii. PLoS ONE 8(7): e6816510.1371/journal.pone.0068165info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-03-06T14:47:26Zoai:www.repository.utl.pt:10400.5/17783Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T17:02:57.353761Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
ZrFsy1, a high-affinity fructose/H+ symporter from fructophilic Zygosaccharomyces rouxii |
title |
ZrFsy1, a high-affinity fructose/H+ symporter from fructophilic Zygosaccharomyces rouxii |
spellingShingle |
ZrFsy1, a high-affinity fructose/H+ symporter from fructophilic Zygosaccharomyces rouxii Leandro, Maria José Zygosaccharomyces rouxii fructose |
title_short |
ZrFsy1, a high-affinity fructose/H+ symporter from fructophilic Zygosaccharomyces rouxii |
title_full |
ZrFsy1, a high-affinity fructose/H+ symporter from fructophilic Zygosaccharomyces rouxii |
title_fullStr |
ZrFsy1, a high-affinity fructose/H+ symporter from fructophilic Zygosaccharomyces rouxii |
title_full_unstemmed |
ZrFsy1, a high-affinity fructose/H+ symporter from fructophilic Zygosaccharomyces rouxii |
title_sort |
ZrFsy1, a high-affinity fructose/H+ symporter from fructophilic Zygosaccharomyces rouxii |
author |
Leandro, Maria José |
author_facet |
Leandro, Maria José Sychrova, Hana Prista, Catarina Loureiro-Dias, M.C. |
author_role |
author |
author2 |
Sychrova, Hana Prista, Catarina Loureiro-Dias, M.C. |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
Repositório da Universidade de Lisboa |
dc.contributor.author.fl_str_mv |
Leandro, Maria José Sychrova, Hana Prista, Catarina Loureiro-Dias, M.C. |
dc.subject.por.fl_str_mv |
Zygosaccharomyces rouxii fructose |
topic |
Zygosaccharomyces rouxii fructose |
description |
Zygosaccharomyces rouxii is a fructophilic yeast than can grow at very high sugar concentrations. We have identified an ORF encoding a putative fructose/H+ symporter in the Z. rouxii CBS 732 genome database. Heterologous expression of this ORF in a S. cerevisiae strain lacking its own hexose transporters (hxt-null) and subsequent kinetic characterization of its sugar transport activity showed it is a high-affinity low-capacity fructose/H+ symporter, with Km 0.4560.07 mM and Vmax 0.5760.02 mmol h21 (gdw) 21. We named it ZrFsy1. This protein also weakly transports xylitol and sorbose, but not glucose or other hexoses. The expression of ZrFSY1 in Z. rouxii is higher when the cells are cultivated at extremely low fructose concentrations (,0.2%) and on non-fermentable carbon sources such as mannitol and xylitol, where the cells have a prolonged lag phase, longer duplication times and change their microscopic morphology. A clear phenotype was determined for the first time for the deletion of a fructose/H+ symporter in the genome where it occurs naturally. The effect of the deletion of ZrFSY1 in Z. rouxii cells is only evident when the cells are cultivated at very low fructose concentrations, when the ZrFsy1 fructose symporter is the main active fructose transporter system |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013 2013-01-01T00:00:00Z 2019-05-09T13:32:42Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.5/17783 |
url |
http://hdl.handle.net/10400.5/17783 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Leandro MJ, Sychrova´ H, Prista C, Loureiro-Dias MC (2013) ZrFsy1, a High-Affinity Fructose/H+ Symporter from Fructophilic Yeast Zygosaccharomyces rouxii. PLoS ONE 8(7): e68165 10.1371/journal.pone.0068165 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
PlosOne |
publisher.none.fl_str_mv |
PlosOne |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
|
_version_ |
1799131119658991616 |