Effects of a sub-lethal copper exposure on non-green Acer pseudoplatanus cell lipids
Autor(a) principal: | |
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Data de Publicação: | 2003 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.21/8880 |
Resumo: | Non-green sycamore cells (Acer pseudoplatanus L.) treated with 50 μM Cu2+ showed a reversible arrest of cell growth after 5 d, indicating that the concentration used was sub-lethal for the cells. Growth arrest was accompanied by hexose and nucleotide-phosphate accumulation and, in contrast with previous reports, no polar-lipid degradation was found. Sub-lethal Cu2+ exposure resulted in an increase in phospholipid amounts, mostly due to an increase of phosphatidylethanolamine. Although the total amount of glycolipids was not significantly changed, Cu2+ exposure changed the relative concentrations of monogalactosyldiacylglycerol (MGDG), digalactosyldiacylglycerol (DGDG) and sulphoquinovosyldiacylglycerol (SQDG). There was a marked increase of DGDG whereas MGDG and SQDG declined. Our results suggest an increase of DGDG synthesis through a pathway similar to the recently described DGD2 pathway and an inhibition of SQDG synthesis. Furthermore, we observed modifications of the fatty acid composition of the plastid synthesized lipids. A significant increase in linoleate (18:2) and a decrease in linolenate (18:3) were found in MGDG, DGDG, SQDG and phosphatidylglycerol (PG), reflecting a deficient desaturation of plastid-located 18:2 fatty acids. In SQDG and PG, Cu2+ treatment also induced a strong enrichment of palmitate (16:0). The effects of Cu2+ on DGDG synthesis and fatty acid desaturation are discussed. |
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Effects of a sub-lethal copper exposure on non-green Acer pseudoplatanus cell lipidsAcer pseudoplatanusCopperMetabolismMembrane lipidsSub-lethal stressAcerNon-green sycamore cells (Acer pseudoplatanus L.) treated with 50 μM Cu2+ showed a reversible arrest of cell growth after 5 d, indicating that the concentration used was sub-lethal for the cells. Growth arrest was accompanied by hexose and nucleotide-phosphate accumulation and, in contrast with previous reports, no polar-lipid degradation was found. Sub-lethal Cu2+ exposure resulted in an increase in phospholipid amounts, mostly due to an increase of phosphatidylethanolamine. Although the total amount of glycolipids was not significantly changed, Cu2+ exposure changed the relative concentrations of monogalactosyldiacylglycerol (MGDG), digalactosyldiacylglycerol (DGDG) and sulphoquinovosyldiacylglycerol (SQDG). There was a marked increase of DGDG whereas MGDG and SQDG declined. Our results suggest an increase of DGDG synthesis through a pathway similar to the recently described DGD2 pathway and an inhibition of SQDG synthesis. Furthermore, we observed modifications of the fatty acid composition of the plastid synthesized lipids. A significant increase in linoleate (18:2) and a decrease in linolenate (18:3) were found in MGDG, DGDG, SQDG and phosphatidylglycerol (PG), reflecting a deficient desaturation of plastid-located 18:2 fatty acids. In SQDG and PG, Cu2+ treatment also induced a strong enrichment of palmitate (16:0). The effects of Cu2+ on DGDG synthesis and fatty acid desaturation are discussed.ElsevierRCIPLPádua, MárioAubert, SergeCasimiro, AdalcinaBligny, RichardBlock, Maryse A2018-09-21T13:21:57Z2003-052003-05-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.21/8880engPádua M, Aubert S, Casimiro A, Bligny R, Block MA. Effects of a sub-lethal copper exposure on non-green Acer pseudoplatanus cell lipids. Plant Physiol Biochem. 2003;41(5):471-7.10.1016/S0981-9428(03)00055-Xinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-08-03T09:56:54Zoai:repositorio.ipl.pt:10400.21/8880Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T20:17:34.703114Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Effects of a sub-lethal copper exposure on non-green Acer pseudoplatanus cell lipids |
title |
Effects of a sub-lethal copper exposure on non-green Acer pseudoplatanus cell lipids |
spellingShingle |
Effects of a sub-lethal copper exposure on non-green Acer pseudoplatanus cell lipids Pádua, Mário Acer pseudoplatanus Copper Metabolism Membrane lipids Sub-lethal stress Acer |
title_short |
Effects of a sub-lethal copper exposure on non-green Acer pseudoplatanus cell lipids |
title_full |
Effects of a sub-lethal copper exposure on non-green Acer pseudoplatanus cell lipids |
title_fullStr |
Effects of a sub-lethal copper exposure on non-green Acer pseudoplatanus cell lipids |
title_full_unstemmed |
Effects of a sub-lethal copper exposure on non-green Acer pseudoplatanus cell lipids |
title_sort |
Effects of a sub-lethal copper exposure on non-green Acer pseudoplatanus cell lipids |
author |
Pádua, Mário |
author_facet |
Pádua, Mário Aubert, Serge Casimiro, Adalcina Bligny, Richard Block, Maryse A |
author_role |
author |
author2 |
Aubert, Serge Casimiro, Adalcina Bligny, Richard Block, Maryse A |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
RCIPL |
dc.contributor.author.fl_str_mv |
Pádua, Mário Aubert, Serge Casimiro, Adalcina Bligny, Richard Block, Maryse A |
dc.subject.por.fl_str_mv |
Acer pseudoplatanus Copper Metabolism Membrane lipids Sub-lethal stress Acer |
topic |
Acer pseudoplatanus Copper Metabolism Membrane lipids Sub-lethal stress Acer |
description |
Non-green sycamore cells (Acer pseudoplatanus L.) treated with 50 μM Cu2+ showed a reversible arrest of cell growth after 5 d, indicating that the concentration used was sub-lethal for the cells. Growth arrest was accompanied by hexose and nucleotide-phosphate accumulation and, in contrast with previous reports, no polar-lipid degradation was found. Sub-lethal Cu2+ exposure resulted in an increase in phospholipid amounts, mostly due to an increase of phosphatidylethanolamine. Although the total amount of glycolipids was not significantly changed, Cu2+ exposure changed the relative concentrations of monogalactosyldiacylglycerol (MGDG), digalactosyldiacylglycerol (DGDG) and sulphoquinovosyldiacylglycerol (SQDG). There was a marked increase of DGDG whereas MGDG and SQDG declined. Our results suggest an increase of DGDG synthesis through a pathway similar to the recently described DGD2 pathway and an inhibition of SQDG synthesis. Furthermore, we observed modifications of the fatty acid composition of the plastid synthesized lipids. A significant increase in linoleate (18:2) and a decrease in linolenate (18:3) were found in MGDG, DGDG, SQDG and phosphatidylglycerol (PG), reflecting a deficient desaturation of plastid-located 18:2 fatty acids. In SQDG and PG, Cu2+ treatment also induced a strong enrichment of palmitate (16:0). The effects of Cu2+ on DGDG synthesis and fatty acid desaturation are discussed. |
publishDate |
2003 |
dc.date.none.fl_str_mv |
2003-05 2003-05-01T00:00:00Z 2018-09-21T13:21:57Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.21/8880 |
url |
http://hdl.handle.net/10400.21/8880 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Pádua M, Aubert S, Casimiro A, Bligny R, Block MA. Effects of a sub-lethal copper exposure on non-green Acer pseudoplatanus cell lipids. Plant Physiol Biochem. 2003;41(5):471-7. 10.1016/S0981-9428(03)00055-X |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799133438635147264 |