PCR-based detection of prey DNA in the gut contents of thr tiger-fly, Coenosia attenuata (Diptera: Muscidae), a biological control agent in Mediterranean greenhouses

Detalhes bibliográficos
Autor(a) principal: Seabra, Sofia G.
Data de Publicação: 2021
Outros Autores: Martins, Joana, Brás, Patrícia, Tavares, Ana M., Freitas, Inês, Barata, António, Rebelo, Maria Teresa, Mateus, Célia, Paulo, Octávio S., Figueiredo, Elisabete
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.5/22689
Resumo: Original article
id RCAP_1bb43ff6060f71ad735e0b99512432f0
oai_identifier_str oai:www.repository.utl.pt:10400.5/22689
network_acronym_str RCAP
network_name_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository_id_str 7160
spelling PCR-based detection of prey DNA in the gut contents of thr tiger-fly, Coenosia attenuata (Diptera: Muscidae), a biological control agent in Mediterranean greenhousesDipteraMuscidaeCoenosia attenuatapredationgut contentsprey molecular identifi cationPolymerase Chain Reactionprobit modelshalf-time molecular detectionOriginal articleThe tiger-fl y Coenosia attenuata Stein (Diptera: Muscidae: Coenosiini) is a generalist predator that preys on several pests of greenhouse crops and is considered a biological control agent in the Mediterranean region. Previous behavioural observations identifi ed its preferred prey, but a more in-depth evaluation will benefi t from using Polymerase Chain Reaction amplifi cation of prey DNA remains in the gut of this predator. To evaluate the rate of decay and suitability of this method for use in the fi eld assessments, we carried out a laboratory feeding calibration experiment on 355 females of C. attenuata, which were killed at different intervals of time after ingestion (10 time points from 0 to 48 h). The prey species tested were: Trialeurodes vaporariorum (Westwood) (Hemiptera: Aleyrodidae: Trialeurodini), Liriomyza huidobrensis (Blanchard) (Diptera: Agromyzidae), Diglyphus isaea (Walker) (Hymenoptera: Eulophidae: Cirrospilini), Bradysia impatiens (Johannsen) (Diptera: Sciaridae) and Drosophila mercatorum Patterson & Wheeler (Diptera: Drosophilidae: Drosophilini). Based on a probit model, amplifi cation success of prey DNA declined exponentially with increasing time after ingestion. The half-time molecular detection differed between species, ranging from an average of 5 h for T. vaporariorum and D. isaea, 6 h for B. impatiens, 15 h for L. huidobrensis to more than 40 h for D. mercatorum. This study confi rmed the feasibility of using DNA based detection to identify prey species in the gut contents of C. attenuata and provided calibration curves for a better understanding of predation activity in this agroecosystemInstitute of Entomology, Biology Centre, CASRepositório da Universidade de LisboaSeabra, Sofia G.Martins, JoanaBrás, PatríciaTavares, Ana M.Freitas, InêsBarata, AntónioRebelo, Maria TeresaMateus, CéliaPaulo, Octávio S.Figueiredo, Elisabete2021-12-10T11:29:57Z20212021-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.5/22689engEur. J. Entomol. 118: 335–343, 202110.14411/eje.2021.035info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-03-06T14:52:15Zoai:www.repository.utl.pt:10400.5/22689Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T17:07:03.198003Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv PCR-based detection of prey DNA in the gut contents of thr tiger-fly, Coenosia attenuata (Diptera: Muscidae), a biological control agent in Mediterranean greenhouses
title PCR-based detection of prey DNA in the gut contents of thr tiger-fly, Coenosia attenuata (Diptera: Muscidae), a biological control agent in Mediterranean greenhouses
spellingShingle PCR-based detection of prey DNA in the gut contents of thr tiger-fly, Coenosia attenuata (Diptera: Muscidae), a biological control agent in Mediterranean greenhouses
Seabra, Sofia G.
Diptera
Muscidae
Coenosia attenuata
predation
gut contents
prey molecular identifi cation
Polymerase Chain Reaction
probit models
half-time molecular detection
title_short PCR-based detection of prey DNA in the gut contents of thr tiger-fly, Coenosia attenuata (Diptera: Muscidae), a biological control agent in Mediterranean greenhouses
title_full PCR-based detection of prey DNA in the gut contents of thr tiger-fly, Coenosia attenuata (Diptera: Muscidae), a biological control agent in Mediterranean greenhouses
title_fullStr PCR-based detection of prey DNA in the gut contents of thr tiger-fly, Coenosia attenuata (Diptera: Muscidae), a biological control agent in Mediterranean greenhouses
title_full_unstemmed PCR-based detection of prey DNA in the gut contents of thr tiger-fly, Coenosia attenuata (Diptera: Muscidae), a biological control agent in Mediterranean greenhouses
title_sort PCR-based detection of prey DNA in the gut contents of thr tiger-fly, Coenosia attenuata (Diptera: Muscidae), a biological control agent in Mediterranean greenhouses
author Seabra, Sofia G.
author_facet Seabra, Sofia G.
Martins, Joana
Brás, Patrícia
Tavares, Ana M.
Freitas, Inês
Barata, António
Rebelo, Maria Teresa
Mateus, Célia
Paulo, Octávio S.
Figueiredo, Elisabete
author_role author
author2 Martins, Joana
Brás, Patrícia
Tavares, Ana M.
Freitas, Inês
Barata, António
Rebelo, Maria Teresa
Mateus, Célia
Paulo, Octávio S.
Figueiredo, Elisabete
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Seabra, Sofia G.
Martins, Joana
Brás, Patrícia
Tavares, Ana M.
Freitas, Inês
Barata, António
Rebelo, Maria Teresa
Mateus, Célia
Paulo, Octávio S.
Figueiredo, Elisabete
dc.subject.por.fl_str_mv Diptera
Muscidae
Coenosia attenuata
predation
gut contents
prey molecular identifi cation
Polymerase Chain Reaction
probit models
half-time molecular detection
topic Diptera
Muscidae
Coenosia attenuata
predation
gut contents
prey molecular identifi cation
Polymerase Chain Reaction
probit models
half-time molecular detection
description Original article
publishDate 2021
dc.date.none.fl_str_mv 2021-12-10T11:29:57Z
2021
2021-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.5/22689
url http://hdl.handle.net/10400.5/22689
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Eur. J. Entomol. 118: 335–343, 2021
10.14411/eje.2021.035
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Institute of Entomology, Biology Centre, CAS
publisher.none.fl_str_mv Institute of Entomology, Biology Centre, CAS
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
_version_ 1799131163790409728

Registros relacionados