Synthesis of gold functionalised nanoparticles with the Eranthis hyemalis lectin and preliminary toxicological studies on Caenorhabditis elegans

Detalhes bibliográficos
Autor(a) principal: Djafari, Jamila
Data de Publicação: 2018
Outros Autores: McConnell, Marie T., Santos, Hugo M., Capelo, José Luis, Bertolo, Emilia, Harvey, Simon C., Lodeiro, Carlos, Fernández-Lodeiro, Javier
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: https://doi.org/10.3390/ma11081363
Resumo: This work was supported by the Associate Laboratory Research Unit for Green Chemistry-Clean Processes and Technologies-LAQV which is financed by national funds from FCT/MEC (UID/QUI/50006/2013) and co-financed by the ERDF under the PT2020 Partnership Agreement (POCI-01-0145-FEDER-007265). E.B. and S.H. are grateful to Canterbury Christ Church University for financial support.
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spelling Synthesis of gold functionalised nanoparticles with the Eranthis hyemalis lectin and preliminary toxicological studies on Caenorhabditis elegansCaenorhabditis elegansGold nanoparticlesLectin proteinNanocompositesReactive Oxygen Species (ROS)ToxicityMaterials Science(all)SDG 3 - Good Health and Well-beingThis work was supported by the Associate Laboratory Research Unit for Green Chemistry-Clean Processes and Technologies-LAQV which is financed by national funds from FCT/MEC (UID/QUI/50006/2013) and co-financed by the ERDF under the PT2020 Partnership Agreement (POCI-01-0145-FEDER-007265). E.B. and S.H. are grateful to Canterbury Christ Church University for financial support.The lectin found in the tubers of the Winter Aconite (Eranthis hyemalis) plant (EHL) is a Type II Ribosome Inactivating Protein (RIP). Type II RIPs have shown anti-cancer properties and have great potential as therapeutic agents. Similarly, colloidal gold nanoparticles are successfully used in biomedical applications as they can be functionalised with ligands with high affinity and specificity for target cells to create therapeutic and imaging agents. Here we present the synthesis and characterization of gold nanoparticles conjugated with EHL and the results of a set of initial assays to establish whether the biological effect of EHL is altered by the conjugation. Gold nanoparticles functionalised with EHL (AuNPs@EHL) were successfully synthesised by bioconjugation with citrate gold nanoparticles (AuNPs@Citrate). The conjugates were analysed by UV-Vis spectroscopy, Dynamic Light Scattering (DLS), Zeta Potential analysis, and Transmission Electron Microscopy (TEM). Results indicate that an optimal functionalisation was achieved with the addition of 100 μL of EHL (concentration 1090 ± 40 μg/mL) over 5 mL of AuNPs (concentration [Au0] = 0.8 mM). Biological assays on the effect of AuNPs@EHL were undertaken on Caenorhabditis elegans, a free-living nematode commonly used for toxicological studies, that has previously been shown to be strongly affected by EHL. Citrate gold nanoparticles did not have any obvious effect on the nematodes. For first larval stage (L1) nematodes, AuNPs@EHL showed a lower biological effect than EHL. For L4 stage, pre-adult nematodes, both EHL alone and AuNPs@EHL delayed the onset of reproduction and reduced fecundity. These assays indicate that EHL can be conjugated to gold nanoparticles and retain elements of biocidal activity.LAQV@REQUIMTEDQ - Departamento de QuímicaRUNDjafari, JamilaMcConnell, Marie T.Santos, Hugo M.Capelo, José LuisBertolo, EmiliaHarvey, Simon C.Lodeiro, CarlosFernández-Lodeiro, Javier2019-01-31T23:45:24Z2018-08-062018-08-06T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://doi.org/10.3390/ma11081363eng1996-1944PURE: 5741085http://www.scopus.com/inward/record.url?scp=85051142009&partnerID=8YFLogxKhttps://doi.org/10.3390/ma11081363info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T04:28:28Zoai:run.unl.pt:10362/59216Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:33:21.542399Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Synthesis of gold functionalised nanoparticles with the Eranthis hyemalis lectin and preliminary toxicological studies on Caenorhabditis elegans
title Synthesis of gold functionalised nanoparticles with the Eranthis hyemalis lectin and preliminary toxicological studies on Caenorhabditis elegans
spellingShingle Synthesis of gold functionalised nanoparticles with the Eranthis hyemalis lectin and preliminary toxicological studies on Caenorhabditis elegans
Djafari, Jamila
Caenorhabditis elegans
Gold nanoparticles
Lectin protein
Nanocomposites
Reactive Oxygen Species (ROS)
Toxicity
Materials Science(all)
SDG 3 - Good Health and Well-being
title_short Synthesis of gold functionalised nanoparticles with the Eranthis hyemalis lectin and preliminary toxicological studies on Caenorhabditis elegans
title_full Synthesis of gold functionalised nanoparticles with the Eranthis hyemalis lectin and preliminary toxicological studies on Caenorhabditis elegans
title_fullStr Synthesis of gold functionalised nanoparticles with the Eranthis hyemalis lectin and preliminary toxicological studies on Caenorhabditis elegans
title_full_unstemmed Synthesis of gold functionalised nanoparticles with the Eranthis hyemalis lectin and preliminary toxicological studies on Caenorhabditis elegans
title_sort Synthesis of gold functionalised nanoparticles with the Eranthis hyemalis lectin and preliminary toxicological studies on Caenorhabditis elegans
author Djafari, Jamila
author_facet Djafari, Jamila
McConnell, Marie T.
Santos, Hugo M.
Capelo, José Luis
Bertolo, Emilia
Harvey, Simon C.
Lodeiro, Carlos
Fernández-Lodeiro, Javier
author_role author
author2 McConnell, Marie T.
Santos, Hugo M.
Capelo, José Luis
Bertolo, Emilia
Harvey, Simon C.
Lodeiro, Carlos
Fernández-Lodeiro, Javier
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv LAQV@REQUIMTE
DQ - Departamento de Química
RUN
dc.contributor.author.fl_str_mv Djafari, Jamila
McConnell, Marie T.
Santos, Hugo M.
Capelo, José Luis
Bertolo, Emilia
Harvey, Simon C.
Lodeiro, Carlos
Fernández-Lodeiro, Javier
dc.subject.por.fl_str_mv Caenorhabditis elegans
Gold nanoparticles
Lectin protein
Nanocomposites
Reactive Oxygen Species (ROS)
Toxicity
Materials Science(all)
SDG 3 - Good Health and Well-being
topic Caenorhabditis elegans
Gold nanoparticles
Lectin protein
Nanocomposites
Reactive Oxygen Species (ROS)
Toxicity
Materials Science(all)
SDG 3 - Good Health and Well-being
description This work was supported by the Associate Laboratory Research Unit for Green Chemistry-Clean Processes and Technologies-LAQV which is financed by national funds from FCT/MEC (UID/QUI/50006/2013) and co-financed by the ERDF under the PT2020 Partnership Agreement (POCI-01-0145-FEDER-007265). E.B. and S.H. are grateful to Canterbury Christ Church University for financial support.
publishDate 2018
dc.date.none.fl_str_mv 2018-08-06
2018-08-06T00:00:00Z
2019-01-31T23:45:24Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://doi.org/10.3390/ma11081363
url https://doi.org/10.3390/ma11081363
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 1996-1944
PURE: 5741085
http://www.scopus.com/inward/record.url?scp=85051142009&partnerID=8YFLogxK
https://doi.org/10.3390/ma11081363
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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