Anatypical active cell death process underlies the fungicidal activity of ciclopiroxolamine against the yeast Saccharomyces cerevisiae

Detalhes bibliográficos
Autor(a) principal: Almeida, Bruno
Data de Publicação: 2007
Outros Autores: Sampaio-Marques, Belém, Carvalho, Joana, Silva, Manuel T., Leão, Cecília, Rodrigues, Fernando José dos Santos, Ludovico, Paula
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/6492
Resumo: Ciclopirox olamine (CPO), a fungicidal agent widely used in clinical practice, induced in Saccharomyces cerevisiae an active cell death (ACD) process characterized by changes in nuclear morphology and chromatin condensation associated with the appearance of a population in the sub-G0/G1 cell cycle phase and an arrest in the G2/M phases. This ACD was associated neither with intracellular reactive oxygen species (ROS) signaling, as revealed by the use of different classes of ROS scavengers, nor with a terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL)-positive phenotype. Furthermore, CPO-induced cell death seems to be dependent on unknown protease activity but independent of the apoptotic regulators Aif1p and Yca1p and of autophagic pathways involving Apg5p, Apg8p and Uth1p. Our results show that CPO triggers in S. cerevisiae an atypical nonapoptotic, nonautophagic ACD with as yet unknown regulators.
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spelling Anatypical active cell death process underlies the fungicidal activity of ciclopiroxolamine against the yeast Saccharomyces cerevisiaeCiclopirox olamineProteasesActive cell deathReactive oxygen speciesCaspase independentcaspase-independent cell deathScience & TechnologyCiclopirox olamine (CPO), a fungicidal agent widely used in clinical practice, induced in Saccharomyces cerevisiae an active cell death (ACD) process characterized by changes in nuclear morphology and chromatin condensation associated with the appearance of a population in the sub-G0/G1 cell cycle phase and an arrest in the G2/M phases. This ACD was associated neither with intracellular reactive oxygen species (ROS) signaling, as revealed by the use of different classes of ROS scavengers, nor with a terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL)-positive phenotype. Furthermore, CPO-induced cell death seems to be dependent on unknown protease activity but independent of the apoptotic regulators Aif1p and Yca1p and of autophagic pathways involving Apg5p, Apg8p and Uth1p. Our results show that CPO triggers in S. cerevisiae an atypical nonapoptotic, nonautophagic ACD with as yet unknown regulators.Oxford University PressUniversidade do MinhoAlmeida, BrunoSampaio-Marques, BelémCarvalho, JoanaSilva, Manuel T.Leão, CecíliaRodrigues, Fernando José dos SantosLudovico, Paula20072007-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/6492engProva tipográfica (In Press)1567-135610.1111/j.1567-1364.2006.00188.x17233764info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:41:57Zoai:repositorium.sdum.uminho.pt:1822/6492Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:39:02.540312Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Anatypical active cell death process underlies the fungicidal activity of ciclopiroxolamine against the yeast Saccharomyces cerevisiae
title Anatypical active cell death process underlies the fungicidal activity of ciclopiroxolamine against the yeast Saccharomyces cerevisiae
spellingShingle Anatypical active cell death process underlies the fungicidal activity of ciclopiroxolamine against the yeast Saccharomyces cerevisiae
Almeida, Bruno
Ciclopirox olamine
Proteases
Active cell death
Reactive oxygen species
Caspase independent
caspase-independent cell death
Science & Technology
title_short Anatypical active cell death process underlies the fungicidal activity of ciclopiroxolamine against the yeast Saccharomyces cerevisiae
title_full Anatypical active cell death process underlies the fungicidal activity of ciclopiroxolamine against the yeast Saccharomyces cerevisiae
title_fullStr Anatypical active cell death process underlies the fungicidal activity of ciclopiroxolamine against the yeast Saccharomyces cerevisiae
title_full_unstemmed Anatypical active cell death process underlies the fungicidal activity of ciclopiroxolamine against the yeast Saccharomyces cerevisiae
title_sort Anatypical active cell death process underlies the fungicidal activity of ciclopiroxolamine against the yeast Saccharomyces cerevisiae
author Almeida, Bruno
author_facet Almeida, Bruno
Sampaio-Marques, Belém
Carvalho, Joana
Silva, Manuel T.
Leão, Cecília
Rodrigues, Fernando José dos Santos
Ludovico, Paula
author_role author
author2 Sampaio-Marques, Belém
Carvalho, Joana
Silva, Manuel T.
Leão, Cecília
Rodrigues, Fernando José dos Santos
Ludovico, Paula
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Almeida, Bruno
Sampaio-Marques, Belém
Carvalho, Joana
Silva, Manuel T.
Leão, Cecília
Rodrigues, Fernando José dos Santos
Ludovico, Paula
dc.subject.por.fl_str_mv Ciclopirox olamine
Proteases
Active cell death
Reactive oxygen species
Caspase independent
caspase-independent cell death
Science & Technology
topic Ciclopirox olamine
Proteases
Active cell death
Reactive oxygen species
Caspase independent
caspase-independent cell death
Science & Technology
description Ciclopirox olamine (CPO), a fungicidal agent widely used in clinical practice, induced in Saccharomyces cerevisiae an active cell death (ACD) process characterized by changes in nuclear morphology and chromatin condensation associated with the appearance of a population in the sub-G0/G1 cell cycle phase and an arrest in the G2/M phases. This ACD was associated neither with intracellular reactive oxygen species (ROS) signaling, as revealed by the use of different classes of ROS scavengers, nor with a terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL)-positive phenotype. Furthermore, CPO-induced cell death seems to be dependent on unknown protease activity but independent of the apoptotic regulators Aif1p and Yca1p and of autophagic pathways involving Apg5p, Apg8p and Uth1p. Our results show that CPO triggers in S. cerevisiae an atypical nonapoptotic, nonautophagic ACD with as yet unknown regulators.
publishDate 2007
dc.date.none.fl_str_mv 2007
2007-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/6492
url http://hdl.handle.net/1822/6492
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Prova tipográfica (In Press)
1567-1356
10.1111/j.1567-1364.2006.00188.x
17233764
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Oxford University Press
publisher.none.fl_str_mv Oxford University Press
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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