Six bacterial vaginosis-associated species can form an in vitro and ex vivo polymicrobial biofilm that is susceptible to Thymbra capitata essential oil

Detalhes bibliográficos
Autor(a) principal: Rosca, Aliona
Data de Publicação: 2022
Outros Autores: Castro, Joana Isabel Reis, Sousa, Lúcia, França, Ângela Maria Oliveira Sousa, Cavaleiro, Carlos, Salgueiro, Lígia, Cerca, Nuno
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: https://hdl.handle.net/1822/82633
Resumo: The Supplementary Material for this article can be found online at: https://www.frontiersin.org/articles/10.3389/fcimb.2022.824860/full#supplementary-material
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spelling Six bacterial vaginosis-associated species can form an in vitro and ex vivo polymicrobial biofilm that is susceptible to Thymbra capitata essential oilbacterial vaginosis (BV)polymicrobial biofilmsalternative therapyessential oilsmicrobial interactionsScience & TechnologyThe Supplementary Material for this article can be found online at: https://www.frontiersin.org/articles/10.3389/fcimb.2022.824860/full#supplementary-materialBacterial vaginosis (BV) is associated with serious gynaecologic and obstetric complications. The hallmark of BV is the presence of a polymicrobial biofilm on the vaginal epithelium, but BV aetiology is still a matter of debate. We have previously developed an in vitro biofilm model that included three BV-associated species, but, up to now, no studies are available whereby more bacterial species are grown together to better mimic the in vivo situation. Herein, we characterized the first polymicrobial BV biofilm consisting of six cultivable BV-associated species by using both in vitro and ex vivo vaginal tissue models. Both models revealed that the six species were able to incorporate the polymicrobial biofilm, at different bacterial concentrations. As it has been thought that this polymicrobial biofilm may increase the survival of BV-associated species when exposed to antibiotics, we also assessed if the Thymbra capitata essential oil (EO), which has recently been shown to be highly bactericidal against several Gardnerella species, could maintain its anti-biofilm activity against this polymicrobial biofilm. Under our experimental conditions, T. capitata EO exhibited a high antibacterial effect against polymicrobial biofilms, in both tested models, with a significant reduction in the biofilm biomass and the number of culturable cells. Overall, this study shows that six BV-associated species can grow together and form a biofilm both in vitro and when using an ex vivo model. Moreover, the data obtained herein should be considered in further applications of T. capitata EO as an antimicrobial agent fighting BV.This work was supported by the Portuguese Foundation for Science and Technology (FCT) by the research project [PTDC/BIA-MIC/28271/2017] under the scope of COMPETE 2020 [POCI-01-0145-FEDER-028271] and by the strategic funding of unit [UID/BIO/04469/2020]. ASR acknowledges the support of FCT individual fellowship [PD/BD/128037/2016]. The founder had no role in the study design and collection, analysis, and interpretation of the results.info:eu-repo/semantics/publishedVersionFrontiers Media S.A.Universidade do MinhoRosca, AlionaCastro, Joana Isabel ReisSousa, LúciaFrança, Ângela Maria Oliveira SousaCavaleiro, CarlosSalgueiro, LígiaCerca, Nuno2022-05-042022-05-04T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/1822/82633engRosca, A. S., Castro, J., Sousa, L. G. V., França, A., Cavaleiro, C., Salgueiro, L., & Cerca, N. (2022, May 4). Six Bacterial Vaginosis-Associated Species Can Form an In Vitro and Ex Vivo Polymicrobial Biofilm That Is Susceptible to Thymbra capitata Essential Oil. Frontiers in Cellular and Infection Microbiology. Frontiers Media SA. http://doi.org/10.3389/fcimb.2022.8248602235-298810.3389/fcimb.2022.82486035601098824860https://www.frontiersin.org/articles/10.3389/fcimb.2022.824860/fullinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:52:02Zoai:repositorium.sdum.uminho.pt:1822/82633Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:51:04.510206Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Six bacterial vaginosis-associated species can form an in vitro and ex vivo polymicrobial biofilm that is susceptible to Thymbra capitata essential oil
title Six bacterial vaginosis-associated species can form an in vitro and ex vivo polymicrobial biofilm that is susceptible to Thymbra capitata essential oil
spellingShingle Six bacterial vaginosis-associated species can form an in vitro and ex vivo polymicrobial biofilm that is susceptible to Thymbra capitata essential oil
Rosca, Aliona
bacterial vaginosis (BV)
polymicrobial biofilms
alternative therapy
essential oils
microbial interactions
Science & Technology
title_short Six bacterial vaginosis-associated species can form an in vitro and ex vivo polymicrobial biofilm that is susceptible to Thymbra capitata essential oil
title_full Six bacterial vaginosis-associated species can form an in vitro and ex vivo polymicrobial biofilm that is susceptible to Thymbra capitata essential oil
title_fullStr Six bacterial vaginosis-associated species can form an in vitro and ex vivo polymicrobial biofilm that is susceptible to Thymbra capitata essential oil
title_full_unstemmed Six bacterial vaginosis-associated species can form an in vitro and ex vivo polymicrobial biofilm that is susceptible to Thymbra capitata essential oil
title_sort Six bacterial vaginosis-associated species can form an in vitro and ex vivo polymicrobial biofilm that is susceptible to Thymbra capitata essential oil
author Rosca, Aliona
author_facet Rosca, Aliona
Castro, Joana Isabel Reis
Sousa, Lúcia
França, Ângela Maria Oliveira Sousa
Cavaleiro, Carlos
Salgueiro, Lígia
Cerca, Nuno
author_role author
author2 Castro, Joana Isabel Reis
Sousa, Lúcia
França, Ângela Maria Oliveira Sousa
Cavaleiro, Carlos
Salgueiro, Lígia
Cerca, Nuno
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Rosca, Aliona
Castro, Joana Isabel Reis
Sousa, Lúcia
França, Ângela Maria Oliveira Sousa
Cavaleiro, Carlos
Salgueiro, Lígia
Cerca, Nuno
dc.subject.por.fl_str_mv bacterial vaginosis (BV)
polymicrobial biofilms
alternative therapy
essential oils
microbial interactions
Science & Technology
topic bacterial vaginosis (BV)
polymicrobial biofilms
alternative therapy
essential oils
microbial interactions
Science & Technology
description The Supplementary Material for this article can be found online at: https://www.frontiersin.org/articles/10.3389/fcimb.2022.824860/full#supplementary-material
publishDate 2022
dc.date.none.fl_str_mv 2022-05-04
2022-05-04T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://hdl.handle.net/1822/82633
url https://hdl.handle.net/1822/82633
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Rosca, A. S., Castro, J., Sousa, L. G. V., França, A., Cavaleiro, C., Salgueiro, L., & Cerca, N. (2022, May 4). Six Bacterial Vaginosis-Associated Species Can Form an In Vitro and Ex Vivo Polymicrobial Biofilm That Is Susceptible to Thymbra capitata Essential Oil. Frontiers in Cellular and Infection Microbiology. Frontiers Media SA. http://doi.org/10.3389/fcimb.2022.824860
2235-2988
10.3389/fcimb.2022.824860
35601098
824860
https://www.frontiersin.org/articles/10.3389/fcimb.2022.824860/full
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Frontiers Media S.A.
publisher.none.fl_str_mv Frontiers Media S.A.
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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