Bioaugmentation with endophytic bacterium E6S homologous to achromobacter piechaudii enhances metal rhizoaccumulation in host sedum plumbizincicola
Autor(a) principal: | |
---|---|
Data de Publicação: | 2016 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.14/23083 |
Resumo: | Application of hyperaccumulator-endophyte symbiotic systems is a potential approach to improve phytoremediation efficiency, since some beneficial endophytic bacteria are able to detoxify heavy metals, alter metal solubility in soil, and facilitate plant growth. The objective of this study was to isolate multi-metal resistant and plant beneficial endophytic bacteria and to evaluate their role in enhancing plant growth and metal accumulation/translocation. The metal resistant endophytic bacterial strain E6S was isolated from stems of the Zn/Cd hyperaccumulator plant Sedum plumbizincicola growing in metalliferous mine soils using Dworkin and Foster salts minimal agar medium with 1-aminocyclopropane-1-carboxylate (ACC) as the sole nitrogen source, and identified as homologous to Achromobacter piechaudii based on morphological and biochemical characteristics, partial 16S rDNA sequence and phylogenetic analysis. Strain E6S showed high level of resistance to various metals (Cd, Zn, and Pb). Besides utilizing ACC, strain E6S exhibited plant beneficial traits, such as solubilization of phosphate and production of indole-3-acetic acid. Inoculation with E6S significantly increased the bioavailability of Cd, Zn, and Pb in soil. In addition, bacterial cells bound considerable amounts of metal ions in the following order: Zn > Cd >Pb. Inoculation of E6S significantly stimulated plant biomass, uptake and bioaccumulation of Cd, Zn, and Pb. However, E6S greatly reduced the root to shoot translocation of Cd and Zn, indicating that bacterial inoculation assisted the host plant to uptake and store heavy metals in its root system. Inoculation with the endophytic bacterium E6S homologous to A. piechaudii can improve phytostabilization of metalliferous soils due to its effective ability to enhance in situ metal rhizoaccumulation in plants. |
id |
RCAP_1c0e24a64279ba5caff083790736ad19 |
---|---|
oai_identifier_str |
oai:repositorio.ucp.pt:10400.14/23083 |
network_acronym_str |
RCAP |
network_name_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository_id_str |
7160 |
spelling |
Bioaugmentation with endophytic bacterium E6S homologous to achromobacter piechaudii enhances metal rhizoaccumulation in host sedum plumbizincicolaEndophytic bacteriumRhizo accumulationMulti-metal contaminationPhytostabilizationSedum plumbizincicolaApplication of hyperaccumulator-endophyte symbiotic systems is a potential approach to improve phytoremediation efficiency, since some beneficial endophytic bacteria are able to detoxify heavy metals, alter metal solubility in soil, and facilitate plant growth. The objective of this study was to isolate multi-metal resistant and plant beneficial endophytic bacteria and to evaluate their role in enhancing plant growth and metal accumulation/translocation. The metal resistant endophytic bacterial strain E6S was isolated from stems of the Zn/Cd hyperaccumulator plant Sedum plumbizincicola growing in metalliferous mine soils using Dworkin and Foster salts minimal agar medium with 1-aminocyclopropane-1-carboxylate (ACC) as the sole nitrogen source, and identified as homologous to Achromobacter piechaudii based on morphological and biochemical characteristics, partial 16S rDNA sequence and phylogenetic analysis. Strain E6S showed high level of resistance to various metals (Cd, Zn, and Pb). Besides utilizing ACC, strain E6S exhibited plant beneficial traits, such as solubilization of phosphate and production of indole-3-acetic acid. Inoculation with E6S significantly increased the bioavailability of Cd, Zn, and Pb in soil. In addition, bacterial cells bound considerable amounts of metal ions in the following order: Zn > Cd >Pb. Inoculation of E6S significantly stimulated plant biomass, uptake and bioaccumulation of Cd, Zn, and Pb. However, E6S greatly reduced the root to shoot translocation of Cd and Zn, indicating that bacterial inoculation assisted the host plant to uptake and store heavy metals in its root system. Inoculation with the endophytic bacterium E6S homologous to A. piechaudii can improve phytostabilization of metalliferous soils due to its effective ability to enhance in situ metal rhizoaccumulation in plants.Frontiers MediaVeritati - Repositório Institucional da Universidade Católica PortuguesaMa, YingZhang, ChangOliveira, Rui S.Freitas, HelenaLuo, Yongming2017-10-13T17:51:02Z20162016-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.14/23083engMA, Ying; ZHANG, Chang; OLIVEIRA, Rui S.; FREITAS, Helena; LUO, Y. - Bioaugmentation with endophytic bacterium E6S homologous to achromobacter piechaudii enhances metal rhizoaccumulation in host sedum plumbizincicola. Frontiers in Plant Science. ISSN 1664-462X. Vol. 7 (2016), 9 p. (75)10.3389/fpls.2016.000751664-462X8495852738326870079000369223100001info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-05T01:35:06Zoai:repositorio.ucp.pt:10400.14/23083Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:17:22.902818Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Bioaugmentation with endophytic bacterium E6S homologous to achromobacter piechaudii enhances metal rhizoaccumulation in host sedum plumbizincicola |
title |
Bioaugmentation with endophytic bacterium E6S homologous to achromobacter piechaudii enhances metal rhizoaccumulation in host sedum plumbizincicola |
spellingShingle |
Bioaugmentation with endophytic bacterium E6S homologous to achromobacter piechaudii enhances metal rhizoaccumulation in host sedum plumbizincicola Ma, Ying Endophytic bacterium Rhizo accumulation Multi-metal contamination Phytostabilization Sedum plumbizincicola |
title_short |
Bioaugmentation with endophytic bacterium E6S homologous to achromobacter piechaudii enhances metal rhizoaccumulation in host sedum plumbizincicola |
title_full |
Bioaugmentation with endophytic bacterium E6S homologous to achromobacter piechaudii enhances metal rhizoaccumulation in host sedum plumbizincicola |
title_fullStr |
Bioaugmentation with endophytic bacterium E6S homologous to achromobacter piechaudii enhances metal rhizoaccumulation in host sedum plumbizincicola |
title_full_unstemmed |
Bioaugmentation with endophytic bacterium E6S homologous to achromobacter piechaudii enhances metal rhizoaccumulation in host sedum plumbizincicola |
title_sort |
Bioaugmentation with endophytic bacterium E6S homologous to achromobacter piechaudii enhances metal rhizoaccumulation in host sedum plumbizincicola |
author |
Ma, Ying |
author_facet |
Ma, Ying Zhang, Chang Oliveira, Rui S. Freitas, Helena Luo, Yongming |
author_role |
author |
author2 |
Zhang, Chang Oliveira, Rui S. Freitas, Helena Luo, Yongming |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Veritati - Repositório Institucional da Universidade Católica Portuguesa |
dc.contributor.author.fl_str_mv |
Ma, Ying Zhang, Chang Oliveira, Rui S. Freitas, Helena Luo, Yongming |
dc.subject.por.fl_str_mv |
Endophytic bacterium Rhizo accumulation Multi-metal contamination Phytostabilization Sedum plumbizincicola |
topic |
Endophytic bacterium Rhizo accumulation Multi-metal contamination Phytostabilization Sedum plumbizincicola |
description |
Application of hyperaccumulator-endophyte symbiotic systems is a potential approach to improve phytoremediation efficiency, since some beneficial endophytic bacteria are able to detoxify heavy metals, alter metal solubility in soil, and facilitate plant growth. The objective of this study was to isolate multi-metal resistant and plant beneficial endophytic bacteria and to evaluate their role in enhancing plant growth and metal accumulation/translocation. The metal resistant endophytic bacterial strain E6S was isolated from stems of the Zn/Cd hyperaccumulator plant Sedum plumbizincicola growing in metalliferous mine soils using Dworkin and Foster salts minimal agar medium with 1-aminocyclopropane-1-carboxylate (ACC) as the sole nitrogen source, and identified as homologous to Achromobacter piechaudii based on morphological and biochemical characteristics, partial 16S rDNA sequence and phylogenetic analysis. Strain E6S showed high level of resistance to various metals (Cd, Zn, and Pb). Besides utilizing ACC, strain E6S exhibited plant beneficial traits, such as solubilization of phosphate and production of indole-3-acetic acid. Inoculation with E6S significantly increased the bioavailability of Cd, Zn, and Pb in soil. In addition, bacterial cells bound considerable amounts of metal ions in the following order: Zn > Cd >Pb. Inoculation of E6S significantly stimulated plant biomass, uptake and bioaccumulation of Cd, Zn, and Pb. However, E6S greatly reduced the root to shoot translocation of Cd and Zn, indicating that bacterial inoculation assisted the host plant to uptake and store heavy metals in its root system. Inoculation with the endophytic bacterium E6S homologous to A. piechaudii can improve phytostabilization of metalliferous soils due to its effective ability to enhance in situ metal rhizoaccumulation in plants. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016 2016-01-01T00:00:00Z 2017-10-13T17:51:02Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.14/23083 |
url |
http://hdl.handle.net/10400.14/23083 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
MA, Ying; ZHANG, Chang; OLIVEIRA, Rui S.; FREITAS, Helena; LUO, Y. - Bioaugmentation with endophytic bacterium E6S homologous to achromobacter piechaudii enhances metal rhizoaccumulation in host sedum plumbizincicola. Frontiers in Plant Science. ISSN 1664-462X. Vol. 7 (2016), 9 p. (75) 10.3389/fpls.2016.00075 1664-462X 84958527383 26870079 000369223100001 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Frontiers Media |
publisher.none.fl_str_mv |
Frontiers Media |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
|
_version_ |
1799131860373078016 |