Adenosine modulation of inhibitory postsynaptic currents mediated by GABAA receptors in rat hippocampal neurons

Detalhes bibliográficos
Autor(a) principal: Rombo, Diogo Miguel Santos
Data de Publicação: 2009
Tipo de documento: Dissertação
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10451/1511
Resumo: Tese de mestrado, Biologia (Biologia Molecular Humana), 2009, Universidade de Lisboa, Faculdade de Ciências
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spelling Adenosine modulation of inhibitory postsynaptic currents mediated by GABAA receptors in rat hippocampal neuronsBiologia celularNeurobiologiaAdenosinaTeses de mestradoTese de mestrado, Biologia (Biologia Molecular Humana), 2009, Universidade de Lisboa, Faculdade de CiênciasResumo alargado em português disponível no documentoMuch of what is known about the modulatory role of adenosine in the hippocampus is on glutamatergic communication and presynaptic GABAergic function. However, any possible role of adenosine on responses of the postsynaptic cell to GABA had not been evaluated. The effect of A1 and A2A receptor activation on inhibitory postsynaptic currents (PSCs) mediated by GABAA receptors was now examined in CA1 pyramidal neurons of young (3-5 weeks) rat hippocampal slices by using the whole-cell patch-clamp technique (Vh=-80mV). GABAA-mediated currents were evoked through pressure application of muscimol (30µM), a GABAA receptor agonist, directly to the cell soma. Bath application of a selective A2A receptor agonist, CGS 21680 (30nM), induced no statistically significant differences on muscimol-evoked currents (101%±2,1% of baseline, n=6, p=0,715). The superfusion of the adenosine A1 receptor agonist, CPA (10-30nM), significantly decreased the peak amplitude of GABAergic responses with a maximum effect (to 54%±3,9% of pre-control value, n=12, p<0.0001) observed within 45 min after its application. This effect of CPA was not reversible within 40min after washing it out from the bath. However, the application of DPCPX (50nM), an adenosine A1 receptor antagonist, induced the recovery of the PSCs towards 84%±6,5% of pre-control value (n=3, p<0,001 compared with CPA (10nM)). Pre-incubation with DPCPX (100nM) also blocked the effect of CPA (30nM) on GABAA receptor currents (100%±0,6% of the baseline, n=5, p=0,503) and unmasked the inhibition of PSCs by endogenous extracellular adenosine (113%±1,0% to 100%±0,6%, n=5, p<0,001). This phenomenon is independent of excitatory inputs arriving to pyramidal CA1 neurons since the CPA effect was maintained (to 60%±8,3%, n=4, p<0,05) in the presence of blockers of excitatory transmission (CNQX (10µM); DL-AP5 (50µM); TTX (500nM)). Together, these results suggest that postsynaptic GABAA-mediated currents are modulated by adenosine A1, but not A2A receptor, in rat hippocampal CA1 pyramidal cells.Sebastião, Ana Maria Ferreira de SousaRamos, Margarida,Repositório da Universidade de LisboaRombo, Diogo Miguel Santos2010-07-27T08:58:46Z20092009-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfapplication/pdftext/xmlhttp://hdl.handle.net/10451/1511enghttp://catalogo.ul.pt/F/?func=item-global&doc_library=ULB01&type=03&doc_number=000576058info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-08T15:41:16Zoai:repositorio.ul.pt:10451/1511Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T21:28:08.326877Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Adenosine modulation of inhibitory postsynaptic currents mediated by GABAA receptors in rat hippocampal neurons
title Adenosine modulation of inhibitory postsynaptic currents mediated by GABAA receptors in rat hippocampal neurons
spellingShingle Adenosine modulation of inhibitory postsynaptic currents mediated by GABAA receptors in rat hippocampal neurons
Rombo, Diogo Miguel Santos
Biologia celular
Neurobiologia
Adenosina
Teses de mestrado
title_short Adenosine modulation of inhibitory postsynaptic currents mediated by GABAA receptors in rat hippocampal neurons
title_full Adenosine modulation of inhibitory postsynaptic currents mediated by GABAA receptors in rat hippocampal neurons
title_fullStr Adenosine modulation of inhibitory postsynaptic currents mediated by GABAA receptors in rat hippocampal neurons
title_full_unstemmed Adenosine modulation of inhibitory postsynaptic currents mediated by GABAA receptors in rat hippocampal neurons
title_sort Adenosine modulation of inhibitory postsynaptic currents mediated by GABAA receptors in rat hippocampal neurons
author Rombo, Diogo Miguel Santos
author_facet Rombo, Diogo Miguel Santos
author_role author
dc.contributor.none.fl_str_mv Sebastião, Ana Maria Ferreira de Sousa
Ramos, Margarida,
Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Rombo, Diogo Miguel Santos
dc.subject.por.fl_str_mv Biologia celular
Neurobiologia
Adenosina
Teses de mestrado
topic Biologia celular
Neurobiologia
Adenosina
Teses de mestrado
description Tese de mestrado, Biologia (Biologia Molecular Humana), 2009, Universidade de Lisboa, Faculdade de Ciências
publishDate 2009
dc.date.none.fl_str_mv 2009
2009-01-01T00:00:00Z
2010-07-27T08:58:46Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10451/1511
url http://hdl.handle.net/10451/1511
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv http://catalogo.ul.pt/F/?func=item-global&doc_library=ULB01&type=03&doc_number=000576058
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
text/xml
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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