Evaluation of the biocontrol potential of a commercial yeast starter against fuel-ethanol fermentation contaminants

Detalhes bibliográficos
Autor(a) principal: Branco, Patricia
Data de Publicação: 2022
Outros Autores: Diniz, Mário, Albergaria, Helena
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.9/3875
Resumo: ABSTRACT: Lactic acid bacteria (LAB) and Brettanomyces bruxellensis are the main contaminants of bioethanol fermentations. Those contaminations affect Saccharomyces cerevisiae performance and reduce ethanol yields and productivity, leading to important economic losses. Currently, chemical treatments such as acid washing and/or antibiotics are used to control those contaminants. However, these control measures carry environmental risks, and more environmentally friendly methods are required. Several S. cerevisiae wine strains were found to secrete antimicrobial peptides (AMPs) during alcoholic fermentation that are active against LAB and B. bruxellensis strains. Thus, in the present study, we investigated if the fuel-ethanol commercial starter S. cerevisiae Ethanol Red (ER) also secretes those AMPs and evaluated its biocontrol potential by performing alcoholic fermentations with mixed-cultures of ER and B. bruxellensis strains and growth assays of LAB in ER pre-fermented supernatants. Results showed that all B. bruxellensis strains were significantly inhibited by the presence of ER, although LAB strains were less sensitive to ER fermentation metabolites. Peptides secreted by ER during alcoholic fermentation were purified by gel-filtration chromatography, and a bioactive fraction was analyzed by ELISA and mass spectrometry. Results confirmed that ER secretes the AMPs previously identified. That bioactive fraction was used to determine minimal inhibitory concentrations (MICs) against several LAB and B. bruxellensis strains. MICs of 1-2 mg/mL were found for B. bruxellensis strains and above 2 mg/mL for LAB. Our study demonstrates that the AMPs secreted by ER can be used as a natural preservative in fuel-ethanol fermentations.
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spelling Evaluation of the biocontrol potential of a commercial yeast starter against fuel-ethanol fermentation contaminantsBioethanolSaccharomyces cerevisiaeAntimicrobial PeptidesAlcoholic fermentationABSTRACT: Lactic acid bacteria (LAB) and Brettanomyces bruxellensis are the main contaminants of bioethanol fermentations. Those contaminations affect Saccharomyces cerevisiae performance and reduce ethanol yields and productivity, leading to important economic losses. Currently, chemical treatments such as acid washing and/or antibiotics are used to control those contaminants. However, these control measures carry environmental risks, and more environmentally friendly methods are required. Several S. cerevisiae wine strains were found to secrete antimicrobial peptides (AMPs) during alcoholic fermentation that are active against LAB and B. bruxellensis strains. Thus, in the present study, we investigated if the fuel-ethanol commercial starter S. cerevisiae Ethanol Red (ER) also secretes those AMPs and evaluated its biocontrol potential by performing alcoholic fermentations with mixed-cultures of ER and B. bruxellensis strains and growth assays of LAB in ER pre-fermented supernatants. Results showed that all B. bruxellensis strains were significantly inhibited by the presence of ER, although LAB strains were less sensitive to ER fermentation metabolites. Peptides secreted by ER during alcoholic fermentation were purified by gel-filtration chromatography, and a bioactive fraction was analyzed by ELISA and mass spectrometry. Results confirmed that ER secretes the AMPs previously identified. That bioactive fraction was used to determine minimal inhibitory concentrations (MICs) against several LAB and B. bruxellensis strains. MICs of 1-2 mg/mL were found for B. bruxellensis strains and above 2 mg/mL for LAB. Our study demonstrates that the AMPs secreted by ER can be used as a natural preservative in fuel-ethanol fermentations.MDPIRepositório do LNEGBranco, PatriciaDiniz, MárioAlbergaria, Helena2022-07-08T14:56:21Z2022-05-01T00:00:00Z2022-05-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.9/3875engBranco, Patrícia... [et.al.] - Evaluation of the biocontrol potential of a commercial yeast starter against fuel-ethanol fermentation contaminants. In: Fermentation, 2022, vol. 8 (5), article nº 23310.3390/fermentation80502332311-5637info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2022-09-06T12:29:44Zoai:repositorio.lneg.pt:10400.9/3875Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T15:37:08.140415Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Evaluation of the biocontrol potential of a commercial yeast starter against fuel-ethanol fermentation contaminants
title Evaluation of the biocontrol potential of a commercial yeast starter against fuel-ethanol fermentation contaminants
spellingShingle Evaluation of the biocontrol potential of a commercial yeast starter against fuel-ethanol fermentation contaminants
Branco, Patricia
Bioethanol
Saccharomyces cerevisiae
Antimicrobial Peptides
Alcoholic fermentation
title_short Evaluation of the biocontrol potential of a commercial yeast starter against fuel-ethanol fermentation contaminants
title_full Evaluation of the biocontrol potential of a commercial yeast starter against fuel-ethanol fermentation contaminants
title_fullStr Evaluation of the biocontrol potential of a commercial yeast starter against fuel-ethanol fermentation contaminants
title_full_unstemmed Evaluation of the biocontrol potential of a commercial yeast starter against fuel-ethanol fermentation contaminants
title_sort Evaluation of the biocontrol potential of a commercial yeast starter against fuel-ethanol fermentation contaminants
author Branco, Patricia
author_facet Branco, Patricia
Diniz, Mário
Albergaria, Helena
author_role author
author2 Diniz, Mário
Albergaria, Helena
author2_role author
author
dc.contributor.none.fl_str_mv Repositório do LNEG
dc.contributor.author.fl_str_mv Branco, Patricia
Diniz, Mário
Albergaria, Helena
dc.subject.por.fl_str_mv Bioethanol
Saccharomyces cerevisiae
Antimicrobial Peptides
Alcoholic fermentation
topic Bioethanol
Saccharomyces cerevisiae
Antimicrobial Peptides
Alcoholic fermentation
description ABSTRACT: Lactic acid bacteria (LAB) and Brettanomyces bruxellensis are the main contaminants of bioethanol fermentations. Those contaminations affect Saccharomyces cerevisiae performance and reduce ethanol yields and productivity, leading to important economic losses. Currently, chemical treatments such as acid washing and/or antibiotics are used to control those contaminants. However, these control measures carry environmental risks, and more environmentally friendly methods are required. Several S. cerevisiae wine strains were found to secrete antimicrobial peptides (AMPs) during alcoholic fermentation that are active against LAB and B. bruxellensis strains. Thus, in the present study, we investigated if the fuel-ethanol commercial starter S. cerevisiae Ethanol Red (ER) also secretes those AMPs and evaluated its biocontrol potential by performing alcoholic fermentations with mixed-cultures of ER and B. bruxellensis strains and growth assays of LAB in ER pre-fermented supernatants. Results showed that all B. bruxellensis strains were significantly inhibited by the presence of ER, although LAB strains were less sensitive to ER fermentation metabolites. Peptides secreted by ER during alcoholic fermentation were purified by gel-filtration chromatography, and a bioactive fraction was analyzed by ELISA and mass spectrometry. Results confirmed that ER secretes the AMPs previously identified. That bioactive fraction was used to determine minimal inhibitory concentrations (MICs) against several LAB and B. bruxellensis strains. MICs of 1-2 mg/mL were found for B. bruxellensis strains and above 2 mg/mL for LAB. Our study demonstrates that the AMPs secreted by ER can be used as a natural preservative in fuel-ethanol fermentations.
publishDate 2022
dc.date.none.fl_str_mv 2022-07-08T14:56:21Z
2022-05-01T00:00:00Z
2022-05-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.9/3875
url http://hdl.handle.net/10400.9/3875
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Branco, Patrícia... [et.al.] - Evaluation of the biocontrol potential of a commercial yeast starter against fuel-ethanol fermentation contaminants. In: Fermentation, 2022, vol. 8 (5), article nº 233
10.3390/fermentation8050233
2311-5637
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
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dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
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