Cryopreservation of cell laden natural origin hydrogels for cartilage regeneration strategies
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/23662 |
Resumo: | The time span needed for obtaining a functional cartilage substitute using tissue engineering strategies, together with the need for specific patient oriented constructs has stimulated the growing interest for developing “off-the shelf” products. One way to deliver such products is based on long-term storage processes, such as cryopreservation, that will provide the clinical substitute available as needed and could be adapted to an autologous immediate solution for the patient. The aim of this study was to examine the effects of cryopreservation on the chondrogenic differentiation characteristics of human mesenchymal derived stem cells isolated from adipose tissue and encapsulated in κ-carrageenan hydrogels. These bioengineered constructs are anticipated to participate in a cartilage regeneration strategy providing temporary habitation for cell survival, proliferation and production of an extracellular matrix which is expected to replace the hydrogel, enhancing the regeneration of native tissues in clinical settings. The results obtained show that the hydrogels withstand the cryopreservation with dimethyl sulfoxide, maintaining their structural integrity, while assisting cells proliferation and chondrogenic potential after cryopreservation. Thus, cell encapsulation systems of natural based hydrogels seem to be an interesting approach for the preservation of cartilage tissue engineered products. |
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Cryopreservation of cell laden natural origin hydrogels for cartilage regeneration strategiesCryopreservationNatural origin polymersHydrogelsCell encapsulationκ-carrageenanScience & TechnologyThe time span needed for obtaining a functional cartilage substitute using tissue engineering strategies, together with the need for specific patient oriented constructs has stimulated the growing interest for developing “off-the shelf” products. One way to deliver such products is based on long-term storage processes, such as cryopreservation, that will provide the clinical substitute available as needed and could be adapted to an autologous immediate solution for the patient. The aim of this study was to examine the effects of cryopreservation on the chondrogenic differentiation characteristics of human mesenchymal derived stem cells isolated from adipose tissue and encapsulated in κ-carrageenan hydrogels. These bioengineered constructs are anticipated to participate in a cartilage regeneration strategy providing temporary habitation for cell survival, proliferation and production of an extracellular matrix which is expected to replace the hydrogel, enhancing the regeneration of native tissues in clinical settings. The results obtained show that the hydrogels withstand the cryopreservation with dimethyl sulfoxide, maintaining their structural integrity, while assisting cells proliferation and chondrogenic potential after cryopreservation. Thus, cell encapsulation systems of natural based hydrogels seem to be an interesting approach for the preservation of cartilage tissue engineered products.We acknowledge financial support to the Portuguese Foundation for Science and Technology (FCT) for the PhD grant SFRH/BD/64070/2009 (EP) and to the project MIT-Portugal Program (MIT/ECE/0047/2009).Royal Society of ChemistryUniversidade do MinhoPopa, Elena GetaRodrigues, Márcia T.Coutinho, Daniela F.Oliveira, Mariana B.Mano, J. F.Reis, R. L.Gomes, Manuela E.20132013-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/23662eng1744-683X10.1039/c2sm26846ainfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:01:14Zoai:repositorium.sdum.uminho.pt:1822/23662Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:51:09.498638Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Cryopreservation of cell laden natural origin hydrogels for cartilage regeneration strategies |
title |
Cryopreservation of cell laden natural origin hydrogels for cartilage regeneration strategies |
spellingShingle |
Cryopreservation of cell laden natural origin hydrogels for cartilage regeneration strategies Popa, Elena Geta Cryopreservation Natural origin polymers Hydrogels Cell encapsulation κ-carrageenan Science & Technology |
title_short |
Cryopreservation of cell laden natural origin hydrogels for cartilage regeneration strategies |
title_full |
Cryopreservation of cell laden natural origin hydrogels for cartilage regeneration strategies |
title_fullStr |
Cryopreservation of cell laden natural origin hydrogels for cartilage regeneration strategies |
title_full_unstemmed |
Cryopreservation of cell laden natural origin hydrogels for cartilage regeneration strategies |
title_sort |
Cryopreservation of cell laden natural origin hydrogels for cartilage regeneration strategies |
author |
Popa, Elena Geta |
author_facet |
Popa, Elena Geta Rodrigues, Márcia T. Coutinho, Daniela F. Oliveira, Mariana B. Mano, J. F. Reis, R. L. Gomes, Manuela E. |
author_role |
author |
author2 |
Rodrigues, Márcia T. Coutinho, Daniela F. Oliveira, Mariana B. Mano, J. F. Reis, R. L. Gomes, Manuela E. |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Popa, Elena Geta Rodrigues, Márcia T. Coutinho, Daniela F. Oliveira, Mariana B. Mano, J. F. Reis, R. L. Gomes, Manuela E. |
dc.subject.por.fl_str_mv |
Cryopreservation Natural origin polymers Hydrogels Cell encapsulation κ-carrageenan Science & Technology |
topic |
Cryopreservation Natural origin polymers Hydrogels Cell encapsulation κ-carrageenan Science & Technology |
description |
The time span needed for obtaining a functional cartilage substitute using tissue engineering strategies, together with the need for specific patient oriented constructs has stimulated the growing interest for developing “off-the shelf” products. One way to deliver such products is based on long-term storage processes, such as cryopreservation, that will provide the clinical substitute available as needed and could be adapted to an autologous immediate solution for the patient. The aim of this study was to examine the effects of cryopreservation on the chondrogenic differentiation characteristics of human mesenchymal derived stem cells isolated from adipose tissue and encapsulated in κ-carrageenan hydrogels. These bioengineered constructs are anticipated to participate in a cartilage regeneration strategy providing temporary habitation for cell survival, proliferation and production of an extracellular matrix which is expected to replace the hydrogel, enhancing the regeneration of native tissues in clinical settings. The results obtained show that the hydrogels withstand the cryopreservation with dimethyl sulfoxide, maintaining their structural integrity, while assisting cells proliferation and chondrogenic potential after cryopreservation. Thus, cell encapsulation systems of natural based hydrogels seem to be an interesting approach for the preservation of cartilage tissue engineered products. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013 2013-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/23662 |
url |
http://hdl.handle.net/1822/23662 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
1744-683X 10.1039/c2sm26846a |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Royal Society of Chemistry |
publisher.none.fl_str_mv |
Royal Society of Chemistry |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1799132282632536064 |