Cryopreservation of cell laden natural origin hydrogels for cartilage regeneration strategies

Detalhes bibliográficos
Autor(a) principal: Popa, Elena Geta
Data de Publicação: 2013
Outros Autores: Rodrigues, Márcia T., Coutinho, Daniela F., Oliveira, Mariana B., Mano, J. F., Reis, R. L., Gomes, Manuela E.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/23662
Resumo: The time span needed for obtaining a functional cartilage substitute using tissue engineering strategies, together with the need for specific patient oriented constructs has stimulated the growing interest for developing “off-the shelf” products. One way to deliver such products is based on long-term storage processes, such as cryopreservation, that will provide the clinical substitute available as needed and could be adapted to an autologous immediate solution for the patient. The aim of this study was to examine the effects of cryopreservation on the chondrogenic differentiation characteristics of human mesenchymal derived stem cells isolated from adipose tissue and encapsulated in κ-carrageenan hydrogels. These bioengineered constructs are anticipated to participate in a cartilage regeneration strategy providing temporary habitation for cell survival, proliferation and production of an extracellular matrix which is expected to replace the hydrogel, enhancing the regeneration of native tissues in clinical settings. The results obtained show that the hydrogels withstand the cryopreservation with dimethyl sulfoxide, maintaining their structural integrity, while assisting cells proliferation and chondrogenic potential after cryopreservation. Thus, cell encapsulation systems of natural based hydrogels seem to be an interesting approach for the preservation of cartilage tissue engineered products.
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spelling Cryopreservation of cell laden natural origin hydrogels for cartilage regeneration strategiesCryopreservationNatural origin polymersHydrogelsCell encapsulationκ-carrageenanScience & TechnologyThe time span needed for obtaining a functional cartilage substitute using tissue engineering strategies, together with the need for specific patient oriented constructs has stimulated the growing interest for developing “off-the shelf” products. One way to deliver such products is based on long-term storage processes, such as cryopreservation, that will provide the clinical substitute available as needed and could be adapted to an autologous immediate solution for the patient. The aim of this study was to examine the effects of cryopreservation on the chondrogenic differentiation characteristics of human mesenchymal derived stem cells isolated from adipose tissue and encapsulated in κ-carrageenan hydrogels. These bioengineered constructs are anticipated to participate in a cartilage regeneration strategy providing temporary habitation for cell survival, proliferation and production of an extracellular matrix which is expected to replace the hydrogel, enhancing the regeneration of native tissues in clinical settings. The results obtained show that the hydrogels withstand the cryopreservation with dimethyl sulfoxide, maintaining their structural integrity, while assisting cells proliferation and chondrogenic potential after cryopreservation. Thus, cell encapsulation systems of natural based hydrogels seem to be an interesting approach for the preservation of cartilage tissue engineered products.We acknowledge financial support to the Portuguese Foundation for Science and Technology (FCT) for the PhD grant SFRH/BD/64070/2009 (EP) and to the project MIT-Portugal Program (MIT/ECE/0047/2009).Royal Society of ChemistryUniversidade do MinhoPopa, Elena GetaRodrigues, Márcia T.Coutinho, Daniela F.Oliveira, Mariana B.Mano, J. F.Reis, R. L.Gomes, Manuela E.20132013-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/23662eng1744-683X10.1039/c2sm26846ainfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:01:14Zoai:repositorium.sdum.uminho.pt:1822/23662Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:51:09.498638Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Cryopreservation of cell laden natural origin hydrogels for cartilage regeneration strategies
title Cryopreservation of cell laden natural origin hydrogels for cartilage regeneration strategies
spellingShingle Cryopreservation of cell laden natural origin hydrogels for cartilage regeneration strategies
Popa, Elena Geta
Cryopreservation
Natural origin polymers
Hydrogels
Cell encapsulation
κ-carrageenan
Science & Technology
title_short Cryopreservation of cell laden natural origin hydrogels for cartilage regeneration strategies
title_full Cryopreservation of cell laden natural origin hydrogels for cartilage regeneration strategies
title_fullStr Cryopreservation of cell laden natural origin hydrogels for cartilage regeneration strategies
title_full_unstemmed Cryopreservation of cell laden natural origin hydrogels for cartilage regeneration strategies
title_sort Cryopreservation of cell laden natural origin hydrogels for cartilage regeneration strategies
author Popa, Elena Geta
author_facet Popa, Elena Geta
Rodrigues, Márcia T.
Coutinho, Daniela F.
Oliveira, Mariana B.
Mano, J. F.
Reis, R. L.
Gomes, Manuela E.
author_role author
author2 Rodrigues, Márcia T.
Coutinho, Daniela F.
Oliveira, Mariana B.
Mano, J. F.
Reis, R. L.
Gomes, Manuela E.
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Popa, Elena Geta
Rodrigues, Márcia T.
Coutinho, Daniela F.
Oliveira, Mariana B.
Mano, J. F.
Reis, R. L.
Gomes, Manuela E.
dc.subject.por.fl_str_mv Cryopreservation
Natural origin polymers
Hydrogels
Cell encapsulation
κ-carrageenan
Science & Technology
topic Cryopreservation
Natural origin polymers
Hydrogels
Cell encapsulation
κ-carrageenan
Science & Technology
description The time span needed for obtaining a functional cartilage substitute using tissue engineering strategies, together with the need for specific patient oriented constructs has stimulated the growing interest for developing “off-the shelf” products. One way to deliver such products is based on long-term storage processes, such as cryopreservation, that will provide the clinical substitute available as needed and could be adapted to an autologous immediate solution for the patient. The aim of this study was to examine the effects of cryopreservation on the chondrogenic differentiation characteristics of human mesenchymal derived stem cells isolated from adipose tissue and encapsulated in κ-carrageenan hydrogels. These bioengineered constructs are anticipated to participate in a cartilage regeneration strategy providing temporary habitation for cell survival, proliferation and production of an extracellular matrix which is expected to replace the hydrogel, enhancing the regeneration of native tissues in clinical settings. The results obtained show that the hydrogels withstand the cryopreservation with dimethyl sulfoxide, maintaining their structural integrity, while assisting cells proliferation and chondrogenic potential after cryopreservation. Thus, cell encapsulation systems of natural based hydrogels seem to be an interesting approach for the preservation of cartilage tissue engineered products.
publishDate 2013
dc.date.none.fl_str_mv 2013
2013-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/23662
url http://hdl.handle.net/1822/23662
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 1744-683X
10.1039/c2sm26846a
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Royal Society of Chemistry
publisher.none.fl_str_mv Royal Society of Chemistry
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
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