The role of Grainy head in epithelial tissue growth

Detalhes bibliográficos
Autor(a) principal: Ponte, Susana Isabel Pereira da
Data de Publicação: 2014
Tipo de documento: Dissertação
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/13839
Resumo: Part of the results of this thesis was presented in the following meetings: Susana Ponte, Lara Carvalho, Inês Cristo and António Jacinto. The role of Grainy head in epithelial tissue growth. Drostuga 2013. Faro, Portugal, January 3rd 2014 [poster] Susana Ponte, Lara Carvalho, Inês Cristo and António Jacinto. The role of Grainy head in epithelial tissue growth. Drostuga 2014. Tomar, Portugal, September 5th-6th 2014 [poster]
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spelling The role of Grainy head in epithelial tissue growthGrainy headEpitheliaGrowthDrosophilaWingWing imaginal discPart of the results of this thesis was presented in the following meetings: Susana Ponte, Lara Carvalho, Inês Cristo and António Jacinto. The role of Grainy head in epithelial tissue growth. Drostuga 2013. Faro, Portugal, January 3rd 2014 [poster] Susana Ponte, Lara Carvalho, Inês Cristo and António Jacinto. The role of Grainy head in epithelial tissue growth. Drostuga 2014. Tomar, Portugal, September 5th-6th 2014 [poster]The grainy head (grh) gene family encodes an important group of transcription factors that play a remarkably conserved role in epithelial organ development, epithelial barrier formation and epithelial repair upon damage in different organisms. The regulation and molecular targets of Grh are numerous and seem to highly depend on the studied developmental context and tissue. Notably, the grh vertebrate homologs, called grh-like (grhl) genes, have recently been implicated in the pathogenesis of several human diseases, including tumor progression and metastasis in different types of cancer. However, the molecular mechanisms by which Grh exerts its function remain largely unknown. The main goal of this project was to investigate the role of Grh in epithelial growth and maintenance using the Drosophila melanogaster (fruit-fly) wing as an in vivo model system. We wanted to understand how Grh influences cell proliferation and apoptosis, as well as cell polarity, cell adhesion and cytoskeleton. Our results show that Grh is essential for epithelial cell survival, since both grh knockdown and overexpression lead to apoptosis. In addition, while grh knockdown induces an increase in cell proliferation, grh overexpression leads to the opposite phenotype, leading us to propose that this gene has a role in the control of cell proliferation. Grh seems to regulate both the expression and the localization of the cell adhesion protein E-cadherin in the wing disc epithelium. We also observed increased F-actin levels upon grh knockdown, suggesting that Grh can influence actin expression or dynamics. In conclusion, our data suggest that Grh is a key transcription factor in the regulation of epithelial maintenance and integrity of the Drosophila wing imaginal disc.Carvalho, LaraRUNPonte, Susana Isabel Pereira da2014-12-02T16:03:40Z2014-092014-122014-09-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfhttp://hdl.handle.net/10362/13839enginfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T03:48:41Zoai:run.unl.pt:10362/13839Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:21:27.103207Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv The role of Grainy head in epithelial tissue growth
title The role of Grainy head in epithelial tissue growth
spellingShingle The role of Grainy head in epithelial tissue growth
Ponte, Susana Isabel Pereira da
Grainy head
Epithelia
Growth
Drosophila
Wing
Wing imaginal disc
title_short The role of Grainy head in epithelial tissue growth
title_full The role of Grainy head in epithelial tissue growth
title_fullStr The role of Grainy head in epithelial tissue growth
title_full_unstemmed The role of Grainy head in epithelial tissue growth
title_sort The role of Grainy head in epithelial tissue growth
author Ponte, Susana Isabel Pereira da
author_facet Ponte, Susana Isabel Pereira da
author_role author
dc.contributor.none.fl_str_mv Carvalho, Lara
RUN
dc.contributor.author.fl_str_mv Ponte, Susana Isabel Pereira da
dc.subject.por.fl_str_mv Grainy head
Epithelia
Growth
Drosophila
Wing
Wing imaginal disc
topic Grainy head
Epithelia
Growth
Drosophila
Wing
Wing imaginal disc
description Part of the results of this thesis was presented in the following meetings: Susana Ponte, Lara Carvalho, Inês Cristo and António Jacinto. The role of Grainy head in epithelial tissue growth. Drostuga 2013. Faro, Portugal, January 3rd 2014 [poster] Susana Ponte, Lara Carvalho, Inês Cristo and António Jacinto. The role of Grainy head in epithelial tissue growth. Drostuga 2014. Tomar, Portugal, September 5th-6th 2014 [poster]
publishDate 2014
dc.date.none.fl_str_mv 2014-12-02T16:03:40Z
2014-09
2014-12
2014-09-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/13839
url http://hdl.handle.net/10362/13839
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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