Spray-drying encapsulation of the live biotherapeutic candidate Akkermansia muciniphila DSM 22959 to survive aerobic storage
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.14/37680 |
Resumo: | Akkermansia muciniphila is regarded as a promising next-generation probiotic or live biotherapeutic candidate. Effective delivery strategies must be developed to ensure high enough viability of the probiotic strain throughout its industrial formulation, distribution chain, shelf-life, and, ultimately, the host’s gastrointestinal tract, where it should exert its beneficial effect(s). Among the possible methodologies, spray-drying is considered industrially attractive regarding its costs, efficiency, and scalability, with the due parameter customization. In this study, spray-drying was explored as a one-step process to encapsulate A. muciniphila DSM 22959, testing the drying settings and three different dairy-based matrices. Microcapsule morphology and size was assessed, and viability throughout storage at 4 or 22 °C and simulated gastrointestinal passage was determined. Akkermansia muciniphila microencapsulation by spray-drying, using 10% skim milk and inlet/outlet temperatures of 150/65 °C, is effective in terms of viability stabilization, both during prolonged aerobic storage and exposure to simulated gastrointestinal passage. Akkermansia muciniphila viability was maintained at around 107 CFU/g up to 28 days at 4 °C under aerobic conditions with viability losses inferior to 1 log reduction. This methodology provides the necessary conditions to efficiently deliver the recommended dose of live A. muciniphila in the human gut as a live biotherapeutic product. |
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Spray-drying encapsulation of the live biotherapeutic candidate Akkermansia muciniphila DSM 22959 to survive aerobic storageAerobic storageAkkermansia muciniphilaMicroencapsulationSimulated gastrointestinal passageSpray-dryingStabilityAkkermansia muciniphila is regarded as a promising next-generation probiotic or live biotherapeutic candidate. Effective delivery strategies must be developed to ensure high enough viability of the probiotic strain throughout its industrial formulation, distribution chain, shelf-life, and, ultimately, the host’s gastrointestinal tract, where it should exert its beneficial effect(s). Among the possible methodologies, spray-drying is considered industrially attractive regarding its costs, efficiency, and scalability, with the due parameter customization. In this study, spray-drying was explored as a one-step process to encapsulate A. muciniphila DSM 22959, testing the drying settings and three different dairy-based matrices. Microcapsule morphology and size was assessed, and viability throughout storage at 4 or 22 °C and simulated gastrointestinal passage was determined. Akkermansia muciniphila microencapsulation by spray-drying, using 10% skim milk and inlet/outlet temperatures of 150/65 °C, is effective in terms of viability stabilization, both during prolonged aerobic storage and exposure to simulated gastrointestinal passage. Akkermansia muciniphila viability was maintained at around 107 CFU/g up to 28 days at 4 °C under aerobic conditions with viability losses inferior to 1 log reduction. This methodology provides the necessary conditions to efficiently deliver the recommended dose of live A. muciniphila in the human gut as a live biotherapeutic product.Veritati - Repositório Institucional da Universidade Católica PortuguesaBarbosa, Joana CristinaAlmeida, DianaMachado, DanielaSousa, SérgioFreitas, Ana CristinaAndrade, José CarlosGomes, Ana Maria2022-05-20T15:44:21Z2022-052022-05-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.14/37680eng1424-824710.3390/ph1505062885130559245PMC914327735631454000802372700001info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-09-06T12:37:31Zoai:repositorio.ucp.pt:10400.14/37680Portal AgregadorONGhttps://www.rcaap.pt/oai/openairemluisa.alvim@gmail.comopendoar:71602024-09-06T12:37:31Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Spray-drying encapsulation of the live biotherapeutic candidate Akkermansia muciniphila DSM 22959 to survive aerobic storage |
title |
Spray-drying encapsulation of the live biotherapeutic candidate Akkermansia muciniphila DSM 22959 to survive aerobic storage |
spellingShingle |
Spray-drying encapsulation of the live biotherapeutic candidate Akkermansia muciniphila DSM 22959 to survive aerobic storage Barbosa, Joana Cristina Aerobic storage Akkermansia muciniphila Microencapsulation Simulated gastrointestinal passage Spray-drying Stability |
title_short |
Spray-drying encapsulation of the live biotherapeutic candidate Akkermansia muciniphila DSM 22959 to survive aerobic storage |
title_full |
Spray-drying encapsulation of the live biotherapeutic candidate Akkermansia muciniphila DSM 22959 to survive aerobic storage |
title_fullStr |
Spray-drying encapsulation of the live biotherapeutic candidate Akkermansia muciniphila DSM 22959 to survive aerobic storage |
title_full_unstemmed |
Spray-drying encapsulation of the live biotherapeutic candidate Akkermansia muciniphila DSM 22959 to survive aerobic storage |
title_sort |
Spray-drying encapsulation of the live biotherapeutic candidate Akkermansia muciniphila DSM 22959 to survive aerobic storage |
author |
Barbosa, Joana Cristina |
author_facet |
Barbosa, Joana Cristina Almeida, Diana Machado, Daniela Sousa, Sérgio Freitas, Ana Cristina Andrade, José Carlos Gomes, Ana Maria |
author_role |
author |
author2 |
Almeida, Diana Machado, Daniela Sousa, Sérgio Freitas, Ana Cristina Andrade, José Carlos Gomes, Ana Maria |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Veritati - Repositório Institucional da Universidade Católica Portuguesa |
dc.contributor.author.fl_str_mv |
Barbosa, Joana Cristina Almeida, Diana Machado, Daniela Sousa, Sérgio Freitas, Ana Cristina Andrade, José Carlos Gomes, Ana Maria |
dc.subject.por.fl_str_mv |
Aerobic storage Akkermansia muciniphila Microencapsulation Simulated gastrointestinal passage Spray-drying Stability |
topic |
Aerobic storage Akkermansia muciniphila Microencapsulation Simulated gastrointestinal passage Spray-drying Stability |
description |
Akkermansia muciniphila is regarded as a promising next-generation probiotic or live biotherapeutic candidate. Effective delivery strategies must be developed to ensure high enough viability of the probiotic strain throughout its industrial formulation, distribution chain, shelf-life, and, ultimately, the host’s gastrointestinal tract, where it should exert its beneficial effect(s). Among the possible methodologies, spray-drying is considered industrially attractive regarding its costs, efficiency, and scalability, with the due parameter customization. In this study, spray-drying was explored as a one-step process to encapsulate A. muciniphila DSM 22959, testing the drying settings and three different dairy-based matrices. Microcapsule morphology and size was assessed, and viability throughout storage at 4 or 22 °C and simulated gastrointestinal passage was determined. Akkermansia muciniphila microencapsulation by spray-drying, using 10% skim milk and inlet/outlet temperatures of 150/65 °C, is effective in terms of viability stabilization, both during prolonged aerobic storage and exposure to simulated gastrointestinal passage. Akkermansia muciniphila viability was maintained at around 107 CFU/g up to 28 days at 4 °C under aerobic conditions with viability losses inferior to 1 log reduction. This methodology provides the necessary conditions to efficiently deliver the recommended dose of live A. muciniphila in the human gut as a live biotherapeutic product. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-05-20T15:44:21Z 2022-05 2022-05-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.14/37680 |
url |
http://hdl.handle.net/10400.14/37680 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
1424-8247 10.3390/ph15050628 85130559245 PMC9143277 35631454 000802372700001 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
mluisa.alvim@gmail.com |
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1817547048869691392 |