Thermoresponsive chitosan–agarose hydrogel for skin regeneration

Detalhes bibliográficos
Autor(a) principal: Miguel, Sónia P.
Data de Publicação: 2014
Outros Autores: Ribeiro, MP., Brancal, Hugo Gonçalo Monteiro Silva Aguiar, Coutinho, Paula Isabel Teixeira Gonçalves, Correia, Ilídio Joaquim Sobreira
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.6/4658
Resumo: Healing enhancement and pain control are critical issues on wound management. So far, different wound dressings have been developed. Among them, hydrogels are the most applied. Herein, a thermoresponsive hydrogel was produced using chitosan (deacetylation degree 95%) and agarose. Hydrogel bactericidal activity, biocompatibility, morphology, porosity and wettability were characterized by confocal microscopy, MTS assay and SEM. The performance of the hydrogel in the wound healing process was evaluated through in vivo assays, during 21 days. The attained results revealed that hydrogel has a pore size (90–400 μm) compatible with cellular internalization and proliferation. A bactericidal activity was observed for hydrogels containing more than 188 μg/mL of chitosan. The improved healing and the lack of a reactive or a granulomatous inflammatory reaction in skin lesions treated with hydrogel demonstrate its suitability to be used in a near future as a wound dressing.
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spelling Thermoresponsive chitosan–agarose hydrogel for skin regenerationAntimicrobial activityBiocompatibilityHydrogelSkin substitutesWound healingHealing enhancement and pain control are critical issues on wound management. So far, different wound dressings have been developed. Among them, hydrogels are the most applied. Herein, a thermoresponsive hydrogel was produced using chitosan (deacetylation degree 95%) and agarose. Hydrogel bactericidal activity, biocompatibility, morphology, porosity and wettability were characterized by confocal microscopy, MTS assay and SEM. The performance of the hydrogel in the wound healing process was evaluated through in vivo assays, during 21 days. The attained results revealed that hydrogel has a pore size (90–400 μm) compatible with cellular internalization and proliferation. A bactericidal activity was observed for hydrogels containing more than 188 μg/mL of chitosan. The improved healing and the lack of a reactive or a granulomatous inflammatory reaction in skin lesions treated with hydrogel demonstrate its suitability to be used in a near future as a wound dressing.ElsevieruBibliorumMiguel, Sónia P.Ribeiro, MP.Brancal, Hugo Gonçalo Monteiro Silva AguiarCoutinho, Paula Isabel Teixeira GonçalvesCorreia, Ilídio Joaquim Sobreira2018-03-20T14:35:04Z2014-05-022014-05-02T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.6/4658engMiguel, S.P., Ribeiro, M.P., Brancal, H., Coutinho, P. e Correia, I.J. (2014) "Thermoresponsive chitosan-agarose hydrogel for skin regeneration", Carbohydrate Polymers, Vol. 111, pp. 366-37310.1016/j.carbpol.2014.04.093metadata only accessinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-12-15T09:41:52Zoai:ubibliorum.ubi.pt:10400.6/4658Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T00:45:43.014453Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Thermoresponsive chitosan–agarose hydrogel for skin regeneration
title Thermoresponsive chitosan–agarose hydrogel for skin regeneration
spellingShingle Thermoresponsive chitosan–agarose hydrogel for skin regeneration
Miguel, Sónia P.
Antimicrobial activity
Biocompatibility
Hydrogel
Skin substitutes
Wound healing
title_short Thermoresponsive chitosan–agarose hydrogel for skin regeneration
title_full Thermoresponsive chitosan–agarose hydrogel for skin regeneration
title_fullStr Thermoresponsive chitosan–agarose hydrogel for skin regeneration
title_full_unstemmed Thermoresponsive chitosan–agarose hydrogel for skin regeneration
title_sort Thermoresponsive chitosan–agarose hydrogel for skin regeneration
author Miguel, Sónia P.
author_facet Miguel, Sónia P.
Ribeiro, MP.
Brancal, Hugo Gonçalo Monteiro Silva Aguiar
Coutinho, Paula Isabel Teixeira Gonçalves
Correia, Ilídio Joaquim Sobreira
author_role author
author2 Ribeiro, MP.
Brancal, Hugo Gonçalo Monteiro Silva Aguiar
Coutinho, Paula Isabel Teixeira Gonçalves
Correia, Ilídio Joaquim Sobreira
author2_role author
author
author
author
dc.contributor.none.fl_str_mv uBibliorum
dc.contributor.author.fl_str_mv Miguel, Sónia P.
Ribeiro, MP.
Brancal, Hugo Gonçalo Monteiro Silva Aguiar
Coutinho, Paula Isabel Teixeira Gonçalves
Correia, Ilídio Joaquim Sobreira
dc.subject.por.fl_str_mv Antimicrobial activity
Biocompatibility
Hydrogel
Skin substitutes
Wound healing
topic Antimicrobial activity
Biocompatibility
Hydrogel
Skin substitutes
Wound healing
description Healing enhancement and pain control are critical issues on wound management. So far, different wound dressings have been developed. Among them, hydrogels are the most applied. Herein, a thermoresponsive hydrogel was produced using chitosan (deacetylation degree 95%) and agarose. Hydrogel bactericidal activity, biocompatibility, morphology, porosity and wettability were characterized by confocal microscopy, MTS assay and SEM. The performance of the hydrogel in the wound healing process was evaluated through in vivo assays, during 21 days. The attained results revealed that hydrogel has a pore size (90–400 μm) compatible with cellular internalization and proliferation. A bactericidal activity was observed for hydrogels containing more than 188 μg/mL of chitosan. The improved healing and the lack of a reactive or a granulomatous inflammatory reaction in skin lesions treated with hydrogel demonstrate its suitability to be used in a near future as a wound dressing.
publishDate 2014
dc.date.none.fl_str_mv 2014-05-02
2014-05-02T00:00:00Z
2018-03-20T14:35:04Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.6/4658
url http://hdl.handle.net/10400.6/4658
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Miguel, S.P., Ribeiro, M.P., Brancal, H., Coutinho, P. e Correia, I.J. (2014) "Thermoresponsive chitosan-agarose hydrogel for skin regeneration", Carbohydrate Polymers, Vol. 111, pp. 366-373
10.1016/j.carbpol.2014.04.093
dc.rights.driver.fl_str_mv metadata only access
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rights_invalid_str_mv metadata only access
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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