Knockout of the PKN family of Rho effector kinases reveals a non-redundant role for PKN2 in developmental mesoderm expansion

Detalhes bibliográficos
Autor(a) principal: Quétier, Ivan
Data de Publicação: 2016
Outros Autores: Marshall, Jacqueline J. T., Spencer-Dene, Bradley, Lachmann, Sylvie, Casamassima, Adele, Franco, Claudio, Escuin, Sarah, Worrall, Joseph T., Baskaran, Priththivika, Rajeeve, Vinothini, Howell, Michael, Copp, Andrew J., Stamp, Gordon, Rosewell, Ian, Cutillas, Pedro, Gerhardt, Holger, Parker, Peter J., Cameron, Angus J. M.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10451/51626
Resumo: Copyright © 2016 The Authors. Published by Elsevier Inc.
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spelling Knockout of the PKN family of Rho effector kinases reveals a non-redundant role for PKN2 in developmental mesoderm expansion4-OHT4-hydroxytamoxifenES cellsEmbryonic stem cellsMEFNCCsPKCPKNProtein kinase NMouse embryonic fibroblastsNeural crest cellsProtein kinase CCopyright © 2016 The Authors. Published by Elsevier Inc.In animals, the protein kinase C (PKC) family has expanded into diversely regulated subgroups, including the Rho family-responsive PKN kinases. Here, we describe knockouts of all three mouse PKN isoforms and reveal that PKN2 loss results in lethality at embryonic day 10 (E10), with associated cardiovascular and morphogenetic defects. The cardiovascular phenotype was not recapitulated by conditional deletion of PKN2 in endothelial cells or the developing heart. In contrast, inducible systemic deletion of PKN2 after E7 provoked collapse of the embryonic mesoderm. Furthermore, mouse embryonic fibroblasts, which arise from the embryonic mesoderm, depend on PKN2 for proliferation and motility. These cellular defects are reflected in vivo as dependence on PKN2 for mesoderm proliferation and neural crest migration. We conclude that failure of the mesoderm to expand in the absence of PKN2 compromises cardiovascular integrity and development, resulting in lethality.ElsevierRepositório da Universidade de LisboaQuétier, IvanMarshall, Jacqueline J. T.Spencer-Dene, BradleyLachmann, SylvieCasamassima, AdeleFranco, ClaudioEscuin, SarahWorrall, Joseph T.Baskaran, PriththivikaRajeeve, VinothiniHowell, MichaelCopp, Andrew J.Stamp, GordonRosewell, IanCutillas, PedroGerhardt, HolgerParker, Peter J.Cameron, Angus J. M.2022-03-07T14:42:21Z20162016-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10451/51626engQuétier et al., 2016, Cell Reports 14, 440-4482211-124710.1016/j.celrep.2015.12.049info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-08T16:56:26Zoai:repositorio.ul.pt:10451/51626Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T22:02:53.225698Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Knockout of the PKN family of Rho effector kinases reveals a non-redundant role for PKN2 in developmental mesoderm expansion
title Knockout of the PKN family of Rho effector kinases reveals a non-redundant role for PKN2 in developmental mesoderm expansion
spellingShingle Knockout of the PKN family of Rho effector kinases reveals a non-redundant role for PKN2 in developmental mesoderm expansion
Quétier, Ivan
4-OHT
4-hydroxytamoxifen
ES cells
Embryonic stem cells
MEF
NCCs
PKC
PKN
Protein kinase N
Mouse embryonic fibroblasts
Neural crest cells
Protein kinase C
title_short Knockout of the PKN family of Rho effector kinases reveals a non-redundant role for PKN2 in developmental mesoderm expansion
title_full Knockout of the PKN family of Rho effector kinases reveals a non-redundant role for PKN2 in developmental mesoderm expansion
title_fullStr Knockout of the PKN family of Rho effector kinases reveals a non-redundant role for PKN2 in developmental mesoderm expansion
title_full_unstemmed Knockout of the PKN family of Rho effector kinases reveals a non-redundant role for PKN2 in developmental mesoderm expansion
title_sort Knockout of the PKN family of Rho effector kinases reveals a non-redundant role for PKN2 in developmental mesoderm expansion
author Quétier, Ivan
author_facet Quétier, Ivan
Marshall, Jacqueline J. T.
Spencer-Dene, Bradley
Lachmann, Sylvie
Casamassima, Adele
Franco, Claudio
Escuin, Sarah
Worrall, Joseph T.
Baskaran, Priththivika
Rajeeve, Vinothini
Howell, Michael
Copp, Andrew J.
Stamp, Gordon
Rosewell, Ian
Cutillas, Pedro
Gerhardt, Holger
Parker, Peter J.
Cameron, Angus J. M.
author_role author
author2 Marshall, Jacqueline J. T.
Spencer-Dene, Bradley
Lachmann, Sylvie
Casamassima, Adele
Franco, Claudio
Escuin, Sarah
Worrall, Joseph T.
Baskaran, Priththivika
Rajeeve, Vinothini
Howell, Michael
Copp, Andrew J.
Stamp, Gordon
Rosewell, Ian
Cutillas, Pedro
Gerhardt, Holger
Parker, Peter J.
Cameron, Angus J. M.
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Quétier, Ivan
Marshall, Jacqueline J. T.
Spencer-Dene, Bradley
Lachmann, Sylvie
Casamassima, Adele
Franco, Claudio
Escuin, Sarah
Worrall, Joseph T.
Baskaran, Priththivika
Rajeeve, Vinothini
Howell, Michael
Copp, Andrew J.
Stamp, Gordon
Rosewell, Ian
Cutillas, Pedro
Gerhardt, Holger
Parker, Peter J.
Cameron, Angus J. M.
dc.subject.por.fl_str_mv 4-OHT
4-hydroxytamoxifen
ES cells
Embryonic stem cells
MEF
NCCs
PKC
PKN
Protein kinase N
Mouse embryonic fibroblasts
Neural crest cells
Protein kinase C
topic 4-OHT
4-hydroxytamoxifen
ES cells
Embryonic stem cells
MEF
NCCs
PKC
PKN
Protein kinase N
Mouse embryonic fibroblasts
Neural crest cells
Protein kinase C
description Copyright © 2016 The Authors. Published by Elsevier Inc.
publishDate 2016
dc.date.none.fl_str_mv 2016
2016-01-01T00:00:00Z
2022-03-07T14:42:21Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10451/51626
url http://hdl.handle.net/10451/51626
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Quétier et al., 2016, Cell Reports 14, 440-448
2211-1247
10.1016/j.celrep.2015.12.049
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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