Overexpression of water-responsive genes promoted by elevated CO2 reduces ROS and enhances drought tolerance in coffea species
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.5/30272 |
Resumo: | Drought is a major constraint to plant growth and productivity worldwide and will aggravate as water availability becomes scarcer. Although elevated air [CO2] might mitigate some of these effects in plants, the mechanisms underlying the involved responses are poorly understood in woody economically important crops such as Coffea. This study analyzed transcriptome changes in Coffea canephora cv. CL153 and C. arabica cv. Icatu exposed to moderate (MWD) or severe water deficits (SWD) and grown under ambient (aCO2) or elevated (eCO2) air [CO2]. We found that changes in expression levels and regulatory pathways were barely affected by MWD, while the SWD condition led to a down-regulation of most differentially expressed genes (DEGs). eCO2 attenuated the impacts of drought in the transcripts of both genotypes but mostly in Icatu, in agreement with physiological and metabolic studies. A predominance of protective and reactive oxygen species (ROS)-scavenging-related genes, directly or indirectly associated with ABA signaling pathways, was found in Coffea responses, including genes involved in water deprivation and desiccation, such as protein phosphatases in Icatu, and aspartic proteases and dehydrins in CL153, whose expression was validated by qRT-PCR. The existence of a complex post-transcriptional regulatory mechanism appears to occur in Coffea explaining some apparent discrepancies between transcriptomic, proteomic, and physiological data in these genotypes. |
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Overexpression of water-responsive genes promoted by elevated CO2 reduces ROS and enhances drought tolerance in coffea speciesABA signalingcoffeefunctional analysisROSstresstoleranceDrought is a major constraint to plant growth and productivity worldwide and will aggravate as water availability becomes scarcer. Although elevated air [CO2] might mitigate some of these effects in plants, the mechanisms underlying the involved responses are poorly understood in woody economically important crops such as Coffea. This study analyzed transcriptome changes in Coffea canephora cv. CL153 and C. arabica cv. Icatu exposed to moderate (MWD) or severe water deficits (SWD) and grown under ambient (aCO2) or elevated (eCO2) air [CO2]. We found that changes in expression levels and regulatory pathways were barely affected by MWD, while the SWD condition led to a down-regulation of most differentially expressed genes (DEGs). eCO2 attenuated the impacts of drought in the transcripts of both genotypes but mostly in Icatu, in agreement with physiological and metabolic studies. A predominance of protective and reactive oxygen species (ROS)-scavenging-related genes, directly or indirectly associated with ABA signaling pathways, was found in Coffea responses, including genes involved in water deprivation and desiccation, such as protein phosphatases in Icatu, and aspartic proteases and dehydrins in CL153, whose expression was validated by qRT-PCR. The existence of a complex post-transcriptional regulatory mechanism appears to occur in Coffea explaining some apparent discrepancies between transcriptomic, proteomic, and physiological data in these genotypes.MDPIRepositório da Universidade de LisboaMarques, IsabelFernandes, IsabelPaulo, Octávio S.Batista, DoraLidon, Fernando C.Partelli, FábioDaMatta, Fábio M.Ribeiro-Barros, Ana I.Ramalho, José C.2024-03-04T17:29:01Z2023-022023-02-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.5/30272engMarques, I.; Fernandes, I.; Paulo, O.S.; Batista, D.; Lidon, F.C.; Partelli, F.; DaMatta, F.M.; Ribeiro-Barros, A.I.; Ramalho, J.C. Overexpression of water-responsive genes promoted by elevated CO2 reduces ROS and enhances drought tolerance in coffea species. Int. J. Mol. Sci. 2023, 24, 3210.10.3390/ijms24043210info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-10T01:34:26Zoai:www.repository.utl.pt:10400.5/30272Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:14:15.468833Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Overexpression of water-responsive genes promoted by elevated CO2 reduces ROS and enhances drought tolerance in coffea species |
title |
Overexpression of water-responsive genes promoted by elevated CO2 reduces ROS and enhances drought tolerance in coffea species |
spellingShingle |
Overexpression of water-responsive genes promoted by elevated CO2 reduces ROS and enhances drought tolerance in coffea species Marques, Isabel ABA signaling coffee functional analysis ROS stress tolerance |
title_short |
Overexpression of water-responsive genes promoted by elevated CO2 reduces ROS and enhances drought tolerance in coffea species |
title_full |
Overexpression of water-responsive genes promoted by elevated CO2 reduces ROS and enhances drought tolerance in coffea species |
title_fullStr |
Overexpression of water-responsive genes promoted by elevated CO2 reduces ROS and enhances drought tolerance in coffea species |
title_full_unstemmed |
Overexpression of water-responsive genes promoted by elevated CO2 reduces ROS and enhances drought tolerance in coffea species |
title_sort |
Overexpression of water-responsive genes promoted by elevated CO2 reduces ROS and enhances drought tolerance in coffea species |
author |
Marques, Isabel |
author_facet |
Marques, Isabel Fernandes, Isabel Paulo, Octávio S. Batista, Dora Lidon, Fernando C. Partelli, Fábio DaMatta, Fábio M. Ribeiro-Barros, Ana I. Ramalho, José C. |
author_role |
author |
author2 |
Fernandes, Isabel Paulo, Octávio S. Batista, Dora Lidon, Fernando C. Partelli, Fábio DaMatta, Fábio M. Ribeiro-Barros, Ana I. Ramalho, José C. |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Repositório da Universidade de Lisboa |
dc.contributor.author.fl_str_mv |
Marques, Isabel Fernandes, Isabel Paulo, Octávio S. Batista, Dora Lidon, Fernando C. Partelli, Fábio DaMatta, Fábio M. Ribeiro-Barros, Ana I. Ramalho, José C. |
dc.subject.por.fl_str_mv |
ABA signaling coffee functional analysis ROS stress tolerance |
topic |
ABA signaling coffee functional analysis ROS stress tolerance |
description |
Drought is a major constraint to plant growth and productivity worldwide and will aggravate as water availability becomes scarcer. Although elevated air [CO2] might mitigate some of these effects in plants, the mechanisms underlying the involved responses are poorly understood in woody economically important crops such as Coffea. This study analyzed transcriptome changes in Coffea canephora cv. CL153 and C. arabica cv. Icatu exposed to moderate (MWD) or severe water deficits (SWD) and grown under ambient (aCO2) or elevated (eCO2) air [CO2]. We found that changes in expression levels and regulatory pathways were barely affected by MWD, while the SWD condition led to a down-regulation of most differentially expressed genes (DEGs). eCO2 attenuated the impacts of drought in the transcripts of both genotypes but mostly in Icatu, in agreement with physiological and metabolic studies. A predominance of protective and reactive oxygen species (ROS)-scavenging-related genes, directly or indirectly associated with ABA signaling pathways, was found in Coffea responses, including genes involved in water deprivation and desiccation, such as protein phosphatases in Icatu, and aspartic proteases and dehydrins in CL153, whose expression was validated by qRT-PCR. The existence of a complex post-transcriptional regulatory mechanism appears to occur in Coffea explaining some apparent discrepancies between transcriptomic, proteomic, and physiological data in these genotypes. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-02 2023-02-01T00:00:00Z 2024-03-04T17:29:01Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.5/30272 |
url |
http://hdl.handle.net/10400.5/30272 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Marques, I.; Fernandes, I.; Paulo, O.S.; Batista, D.; Lidon, F.C.; Partelli, F.; DaMatta, F.M.; Ribeiro-Barros, A.I.; Ramalho, J.C. Overexpression of water-responsive genes promoted by elevated CO2 reduces ROS and enhances drought tolerance in coffea species. Int. J. Mol. Sci. 2023, 24, 3210. 10.3390/ijms24043210 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
MDPI |
publisher.none.fl_str_mv |
MDPI |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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