SNAREs in Mammalian Sperm: Possible Implications for Fertilization
Autor(a) principal: | |
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Data de Publicação: | 2000 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10316/5444 |
Resumo: | Soluble N-ethylmalameide-sensitive factor attachment protein receptor (SNARE) proteins are present in mammalian sperm and could be involved in critical membrane fusion events during fertilization, namely the acrosome reaction. Vesicle-associated membrane protein/synaptobrevin, a SNARE on the membrane of a vesicular carrier, and syntaxin 1, a SNARE on the target membrane, as well as the calcium sensor synaptotagmin I, are present in the acrosome of mammalian sperm (human, rhesus monkey, bull, hamster, mouse). Sperm SNAREs are sloughed off during the acrosome reaction, paralleling the release of sperm membrane vesicles and acrosomal contents, and SNARE antibodies inhibit both the acrosome reaction and fertilization, without inhibiting sperm-egg binding. In addition, sperm SNAREs may be responsible, together with other sperm components, for the asynchronous male DNA decondensation that occurs following intracytoplasmic sperm injection, an assisted reproduction technique that bypasses normal sperm-egg surface interactions. The results suggest the participation of sperm SNAREs during membrane fusion events at fertilization in mammals. |
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SNAREs in Mammalian Sperm: Possible Implications for Fertilizationsperm; fertilization; membrane; exocytosis; acrosomeSoluble N-ethylmalameide-sensitive factor attachment protein receptor (SNARE) proteins are present in mammalian sperm and could be involved in critical membrane fusion events during fertilization, namely the acrosome reaction. Vesicle-associated membrane protein/synaptobrevin, a SNARE on the membrane of a vesicular carrier, and syntaxin 1, a SNARE on the target membrane, as well as the calcium sensor synaptotagmin I, are present in the acrosome of mammalian sperm (human, rhesus monkey, bull, hamster, mouse). Sperm SNAREs are sloughed off during the acrosome reaction, paralleling the release of sperm membrane vesicles and acrosomal contents, and SNARE antibodies inhibit both the acrosome reaction and fertilization, without inhibiting sperm-egg binding. In addition, sperm SNAREs may be responsible, together with other sperm components, for the asynchronous male DNA decondensation that occurs following intracytoplasmic sperm injection, an assisted reproduction technique that bypasses normal sperm-egg surface interactions. The results suggest the participation of sperm SNAREs during membrane fusion events at fertilization in mammals.http://www.sciencedirect.com/science/article/B6WDG-45F53DF-6N/1/049af5c994522859c35001d18bc0f94c2000info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleaplication/PDFhttp://hdl.handle.net/10316/5444http://hdl.handle.net/10316/5444engDevelopmental Biology. 223:1 (2000) 54-69Ramalho-Santos, JoãoMoreno, Ricardo D.Sutovsky, PeterChan, Anthony Wing-SangHewitson, LauraWessel, Gary M.Simerly, Calvin R.Schatten, Geraldinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2020-05-27T16:14:54Zoai:estudogeral.uc.pt:10316/5444Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T20:55:30.192753Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
SNAREs in Mammalian Sperm: Possible Implications for Fertilization |
title |
SNAREs in Mammalian Sperm: Possible Implications for Fertilization |
spellingShingle |
SNAREs in Mammalian Sperm: Possible Implications for Fertilization Ramalho-Santos, João sperm; fertilization; membrane; exocytosis; acrosome |
title_short |
SNAREs in Mammalian Sperm: Possible Implications for Fertilization |
title_full |
SNAREs in Mammalian Sperm: Possible Implications for Fertilization |
title_fullStr |
SNAREs in Mammalian Sperm: Possible Implications for Fertilization |
title_full_unstemmed |
SNAREs in Mammalian Sperm: Possible Implications for Fertilization |
title_sort |
SNAREs in Mammalian Sperm: Possible Implications for Fertilization |
author |
Ramalho-Santos, João |
author_facet |
Ramalho-Santos, João Moreno, Ricardo D. Sutovsky, Peter Chan, Anthony Wing-Sang Hewitson, Laura Wessel, Gary M. Simerly, Calvin R. Schatten, Gerald |
author_role |
author |
author2 |
Moreno, Ricardo D. Sutovsky, Peter Chan, Anthony Wing-Sang Hewitson, Laura Wessel, Gary M. Simerly, Calvin R. Schatten, Gerald |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Ramalho-Santos, João Moreno, Ricardo D. Sutovsky, Peter Chan, Anthony Wing-Sang Hewitson, Laura Wessel, Gary M. Simerly, Calvin R. Schatten, Gerald |
dc.subject.por.fl_str_mv |
sperm; fertilization; membrane; exocytosis; acrosome |
topic |
sperm; fertilization; membrane; exocytosis; acrosome |
description |
Soluble N-ethylmalameide-sensitive factor attachment protein receptor (SNARE) proteins are present in mammalian sperm and could be involved in critical membrane fusion events during fertilization, namely the acrosome reaction. Vesicle-associated membrane protein/synaptobrevin, a SNARE on the membrane of a vesicular carrier, and syntaxin 1, a SNARE on the target membrane, as well as the calcium sensor synaptotagmin I, are present in the acrosome of mammalian sperm (human, rhesus monkey, bull, hamster, mouse). Sperm SNAREs are sloughed off during the acrosome reaction, paralleling the release of sperm membrane vesicles and acrosomal contents, and SNARE antibodies inhibit both the acrosome reaction and fertilization, without inhibiting sperm-egg binding. In addition, sperm SNAREs may be responsible, together with other sperm components, for the asynchronous male DNA decondensation that occurs following intracytoplasmic sperm injection, an assisted reproduction technique that bypasses normal sperm-egg surface interactions. The results suggest the participation of sperm SNAREs during membrane fusion events at fertilization in mammals. |
publishDate |
2000 |
dc.date.none.fl_str_mv |
2000 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10316/5444 http://hdl.handle.net/10316/5444 |
url |
http://hdl.handle.net/10316/5444 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Developmental Biology. 223:1 (2000) 54-69 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
aplication/PDF |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
institution |
RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799133842168086528 |