Selective Coordination of Cu2+ and Subsequent Anion Detection Based on a Naphthalimide-Triazine-(DPA)2 Chemosensor

Detalhes bibliográficos
Autor(a) principal: Moro, Artur J.
Data de Publicação: 2020
Outros Autores: Santos, Miguel, Outis, Mani, Mateus, Pedro, Pereira, Pedro Matos
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/116889
Resumo: UIDB/50006/2020 SFRH/BD/120985/2016 Project LISBOA-01-0145-FEDER-007660 PPBI-POCI-01-0145-FEDER-022122 DL 57-021/DL/2018 ROTEIRO/0031/2013-PINFRA/22161/2016
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spelling Selective Coordination of Cu2+ and Subsequent Anion Detection Based on a Naphthalimide-Triazine-(DPA)2 Chemosensorcopper (II)fluorescent chemosensornaphthalimidephosphate derivativessuper-resolution fluorescence microscopyClinical BiochemistryUIDB/50006/2020 SFRH/BD/120985/2016 Project LISBOA-01-0145-FEDER-007660 PPBI-POCI-01-0145-FEDER-022122 DL 57-021/DL/2018 ROTEIRO/0031/2013-PINFRA/22161/2016A new fluorescent chemosensor for copper (II) and subsequent anion sensing was designed and fully characterized. The sensor consisted of a 1,8-naphthalimide core, bearing two terminal dipicolylamine (DPA) receptor units for binding metal cations, and an ethoxyethanol moiety for enhanced water solubility. The DPA units are connected to position 4 of the fluorophore via a triazine-ethylenediamine spacer. Fluorescence titration studies of the chemosensor revealed a high selectivity for Cu2+ over other divalent ions, the emissions were strongly quenched upon binding, and a stability constant of 5.52 log units was obtained. Given the distance from DPA chelating units and the fluorophore, quenching from the Cu2+ complexation suggests an electron transfer or an electronic energy transfer mechanism. Furthermore, the Cu2+-sensor complex proved to be capable of sensing anionic phosphate derivatives through the displacement of the Cu2+ cation, which translated into a full recovery of the luminescence from the naphthalimide. Super-resolution fluorescence microscopy studies performed in HeLa cells showed there was a high intracellular uptake of the chemosensor. Incubation in Cu2+ spiked media revealed a strong fluorescent signal from mitochondria and cell membranes, which is consistent with a high concentration of ATP at these intracellular sites.LAQV@REQUIMTEDQ - Departamento de QuímicaInstituto de Tecnologia Química e Biológica António Xavier (ITQB)RUNMoro, Artur J.Santos, MiguelOutis, ManiMateus, PedroPereira, Pedro Matos2021-05-03T22:53:20Z2020-09-222020-09-22T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10362/116889eng2079-6374PURE: 20124686https://doi.org/10.3390/bios10090129info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T04:59:45Zoai:run.unl.pt:10362/116889Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:43:21.122511Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Selective Coordination of Cu2+ and Subsequent Anion Detection Based on a Naphthalimide-Triazine-(DPA)2 Chemosensor
title Selective Coordination of Cu2+ and Subsequent Anion Detection Based on a Naphthalimide-Triazine-(DPA)2 Chemosensor
spellingShingle Selective Coordination of Cu2+ and Subsequent Anion Detection Based on a Naphthalimide-Triazine-(DPA)2 Chemosensor
Moro, Artur J.
copper (II)
fluorescent chemosensor
naphthalimide
phosphate derivatives
super-resolution fluorescence microscopy
Clinical Biochemistry
title_short Selective Coordination of Cu2+ and Subsequent Anion Detection Based on a Naphthalimide-Triazine-(DPA)2 Chemosensor
title_full Selective Coordination of Cu2+ and Subsequent Anion Detection Based on a Naphthalimide-Triazine-(DPA)2 Chemosensor
title_fullStr Selective Coordination of Cu2+ and Subsequent Anion Detection Based on a Naphthalimide-Triazine-(DPA)2 Chemosensor
title_full_unstemmed Selective Coordination of Cu2+ and Subsequent Anion Detection Based on a Naphthalimide-Triazine-(DPA)2 Chemosensor
title_sort Selective Coordination of Cu2+ and Subsequent Anion Detection Based on a Naphthalimide-Triazine-(DPA)2 Chemosensor
author Moro, Artur J.
author_facet Moro, Artur J.
Santos, Miguel
Outis, Mani
Mateus, Pedro
Pereira, Pedro Matos
author_role author
author2 Santos, Miguel
Outis, Mani
Mateus, Pedro
Pereira, Pedro Matos
author2_role author
author
author
author
dc.contributor.none.fl_str_mv LAQV@REQUIMTE
DQ - Departamento de Química
Instituto de Tecnologia Química e Biológica António Xavier (ITQB)
RUN
dc.contributor.author.fl_str_mv Moro, Artur J.
Santos, Miguel
Outis, Mani
Mateus, Pedro
Pereira, Pedro Matos
dc.subject.por.fl_str_mv copper (II)
fluorescent chemosensor
naphthalimide
phosphate derivatives
super-resolution fluorescence microscopy
Clinical Biochemistry
topic copper (II)
fluorescent chemosensor
naphthalimide
phosphate derivatives
super-resolution fluorescence microscopy
Clinical Biochemistry
description UIDB/50006/2020 SFRH/BD/120985/2016 Project LISBOA-01-0145-FEDER-007660 PPBI-POCI-01-0145-FEDER-022122 DL 57-021/DL/2018 ROTEIRO/0031/2013-PINFRA/22161/2016
publishDate 2020
dc.date.none.fl_str_mv 2020-09-22
2020-09-22T00:00:00Z
2021-05-03T22:53:20Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/116889
url http://hdl.handle.net/10362/116889
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 2079-6374
PURE: 20124686
https://doi.org/10.3390/bios10090129
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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