Microbiota-dependent expansion of testicular IL-17-producing Vγ6+ γδ T cells upon puberty promotes local tissue immune surveillance

Detalhes bibliográficos
Autor(a) principal: Wilharm, Anneke
Data de Publicação: 2020
Outros Autores: Cristiano Brigas, Maria Helena, Sandrock, Inga, Ribeiro, Miguel, Amado, Tiago, Reinhardt, Annika, Demera, Abdi, Hoenicke, Lisa, Strowig, Till, Carvalho, Tânia, Prinz, Immo, Ribot, Julie
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10451/50244
Resumo: © The Author(s) 2020. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
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spelling Microbiota-dependent expansion of testicular IL-17-producing Vγ6+ γδ T cells upon puberty promotes local tissue immune surveillance© The Author(s) 2020. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.γδT cells represent the majority of lymphocytes in several mucosal tissues where they contribute to tissue homoeostasis, microbial defence and wound repair. Here we characterise a population of interleukin (IL) 17-producing γδ (γδ17) T cells that seed the testis of naive C57BL/6 mice, expand at puberty and persist throughout adulthood. We show that this population is foetal-derived and displays a T-cell receptor (TCR) repertoire highly biased towards Vγ6-containing rearrangements. These γδ17 cells were the major source of IL-17 in the testis, whereas αβ T cells mostly provided interferon (IFN)-γ in situ. Importantly, testicular γδ17 cell homoeostasis was strongly dependent on the microbiota and Toll-like receptor (TLR4)/IL-1α/IL-23 signalling. We further found that γδ17 cells contributed to tissue surveillance in a model of experimental orchitis induced by intra-testicular inoculation of Listeria monocytogenes, as Tcrδ-/- and Il17-/- infected mice displayed higher bacterial loads than wild-type (WT) controls and died 3 days after infection. Altogether, this study identified a previously unappreciated foetal-derived γδ17 cell subset that infiltrates the testis at steady state, expands upon puberty and plays a crucial role in local tissue immune surveillance.This work was funded by the Fundação para a Ciência e Tecnologia (IF/00013/2014 to J.C.R., PD/BD/114103/2015 to H.C.B.), and by the Deutsche Forschungsgemeinschaft (DFG) grants PR727/8-1 and PR727/11-1 to I.P.; A.W. was a scholar of Hannover Biomedical research School. This publication was supported by LISBOA-01-0145-FEDER-028241, project funded by Fundação para a Ciência e a Tecnologia (FCT) and Fundos Europeus Estruturais e de Investimento through POR Lisboa 2020 (Programa Operacional Regional de Lisboa, do Portugal 2020).Springer NatureRepositório da Universidade de LisboaWilharm, AnnekeCristiano Brigas, Maria HelenaSandrock, IngaRibeiro, MiguelAmado, TiagoReinhardt, AnnikaDemera, AbdiHoenicke, LisaStrowig, TillCarvalho, TâniaPrinz, ImmoRibot, Julie2021-12-02T14:42:25Z20202020-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10451/50244engMucosal Immunol 14, 242–252 (2021)1933-021910.1038/s41385-020-0330-61935-3456info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-08T16:54:15Zoai:repositorio.ul.pt:10451/50244Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T22:01:41.308145Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Microbiota-dependent expansion of testicular IL-17-producing Vγ6+ γδ T cells upon puberty promotes local tissue immune surveillance
title Microbiota-dependent expansion of testicular IL-17-producing Vγ6+ γδ T cells upon puberty promotes local tissue immune surveillance
spellingShingle Microbiota-dependent expansion of testicular IL-17-producing Vγ6+ γδ T cells upon puberty promotes local tissue immune surveillance
Wilharm, Anneke
title_short Microbiota-dependent expansion of testicular IL-17-producing Vγ6+ γδ T cells upon puberty promotes local tissue immune surveillance
title_full Microbiota-dependent expansion of testicular IL-17-producing Vγ6+ γδ T cells upon puberty promotes local tissue immune surveillance
title_fullStr Microbiota-dependent expansion of testicular IL-17-producing Vγ6+ γδ T cells upon puberty promotes local tissue immune surveillance
title_full_unstemmed Microbiota-dependent expansion of testicular IL-17-producing Vγ6+ γδ T cells upon puberty promotes local tissue immune surveillance
title_sort Microbiota-dependent expansion of testicular IL-17-producing Vγ6+ γδ T cells upon puberty promotes local tissue immune surveillance
author Wilharm, Anneke
author_facet Wilharm, Anneke
Cristiano Brigas, Maria Helena
Sandrock, Inga
Ribeiro, Miguel
Amado, Tiago
Reinhardt, Annika
Demera, Abdi
Hoenicke, Lisa
Strowig, Till
Carvalho, Tânia
Prinz, Immo
Ribot, Julie
author_role author
author2 Cristiano Brigas, Maria Helena
Sandrock, Inga
Ribeiro, Miguel
Amado, Tiago
Reinhardt, Annika
Demera, Abdi
Hoenicke, Lisa
Strowig, Till
Carvalho, Tânia
Prinz, Immo
Ribot, Julie
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Wilharm, Anneke
Cristiano Brigas, Maria Helena
Sandrock, Inga
Ribeiro, Miguel
Amado, Tiago
Reinhardt, Annika
Demera, Abdi
Hoenicke, Lisa
Strowig, Till
Carvalho, Tânia
Prinz, Immo
Ribot, Julie
description © The Author(s) 2020. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
publishDate 2020
dc.date.none.fl_str_mv 2020
2020-01-01T00:00:00Z
2021-12-02T14:42:25Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
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dc.relation.none.fl_str_mv Mucosal Immunol 14, 242–252 (2021)
1933-0219
10.1038/s41385-020-0330-6
1935-3456
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