Voltammetric monitoring of laccase-catalysed mediated reactions

Detalhes bibliográficos
Autor(a) principal: Fernández-Sánchez, César
Data de Publicação: 2002
Outros Autores: Tzanov, Tzanko, Gübitz, Georg M., Paulo, Artur Cavaco
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/1940
Resumo: Six different compounds capable of mediating laccase-catalysed reactions have been tested by cyclic voltammetry. They exhibited quasi-reversible electrodic behaviour with formal redox potentials ranging from 150 to 800 mV (E-0t vs. SCE). The immersion of a laccase-coated glassy carbon electrode (GCE) in mediator solutions generated large cathodic catalytic currents easily recorded by cyclic voltammetry at low-potential scan rates. This current showed two well-defined pH profiles, which correlated with the variation of the mediator redox potentials at the pH range tested. The relevant effect of temperature on the activity of laccase has been assessed here. Likewise, it was shown that the cut-rent record varied with the substrate concentration. This trend fitted Michaelis-Menten kinetics, which allowed us to give an estimation of the affinity of the fungal laccase for the different mediators. (C) 2002 Elsevier Science B.V. All rights reserved.
id RCAP_206e8ece1fdd00d0165df9cb406adb7a
oai_identifier_str oai:repositorium.sdum.uminho.pt:1822/1940
network_acronym_str RCAP
network_name_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository_id_str 7160
spelling Voltammetric monitoring of laccase-catalysed mediated reactionslaccaseimmobilisationmediatorcyclic voltammetrycatalytic currentScience & TechnologySix different compounds capable of mediating laccase-catalysed reactions have been tested by cyclic voltammetry. They exhibited quasi-reversible electrodic behaviour with formal redox potentials ranging from 150 to 800 mV (E-0t vs. SCE). The immersion of a laccase-coated glassy carbon electrode (GCE) in mediator solutions generated large cathodic catalytic currents easily recorded by cyclic voltammetry at low-potential scan rates. This current showed two well-defined pH profiles, which correlated with the variation of the mediator redox potentials at the pH range tested. The relevant effect of temperature on the activity of laccase has been assessed here. Likewise, it was shown that the cut-rent record varied with the substrate concentration. This trend fitted Michaelis-Menten kinetics, which allowed us to give an estimation of the affinity of the fungal laccase for the different mediators. (C) 2002 Elsevier Science B.V. All rights reserved.ElsevierUniversidade do MinhoFernández-Sánchez, CésarTzanov, TzankoGübitz, Georg M.Paulo, Artur Cavaco20022002-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/1940eng"Bioelectrochemistry". ISSN 1567-5394. 58 (2002) 149-156.1567-539410.1016/S1567-5394(02)00119-612414320info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:21:38Zoai:repositorium.sdum.uminho.pt:1822/1940Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:14:56.976584Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Voltammetric monitoring of laccase-catalysed mediated reactions
title Voltammetric monitoring of laccase-catalysed mediated reactions
spellingShingle Voltammetric monitoring of laccase-catalysed mediated reactions
Fernández-Sánchez, César
laccase
immobilisation
mediator
cyclic voltammetry
catalytic current
Science & Technology
title_short Voltammetric monitoring of laccase-catalysed mediated reactions
title_full Voltammetric monitoring of laccase-catalysed mediated reactions
title_fullStr Voltammetric monitoring of laccase-catalysed mediated reactions
title_full_unstemmed Voltammetric monitoring of laccase-catalysed mediated reactions
title_sort Voltammetric monitoring of laccase-catalysed mediated reactions
author Fernández-Sánchez, César
author_facet Fernández-Sánchez, César
Tzanov, Tzanko
Gübitz, Georg M.
Paulo, Artur Cavaco
author_role author
author2 Tzanov, Tzanko
Gübitz, Georg M.
Paulo, Artur Cavaco
author2_role author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Fernández-Sánchez, César
Tzanov, Tzanko
Gübitz, Georg M.
Paulo, Artur Cavaco
dc.subject.por.fl_str_mv laccase
immobilisation
mediator
cyclic voltammetry
catalytic current
Science & Technology
topic laccase
immobilisation
mediator
cyclic voltammetry
catalytic current
Science & Technology
description Six different compounds capable of mediating laccase-catalysed reactions have been tested by cyclic voltammetry. They exhibited quasi-reversible electrodic behaviour with formal redox potentials ranging from 150 to 800 mV (E-0t vs. SCE). The immersion of a laccase-coated glassy carbon electrode (GCE) in mediator solutions generated large cathodic catalytic currents easily recorded by cyclic voltammetry at low-potential scan rates. This current showed two well-defined pH profiles, which correlated with the variation of the mediator redox potentials at the pH range tested. The relevant effect of temperature on the activity of laccase has been assessed here. Likewise, it was shown that the cut-rent record varied with the substrate concentration. This trend fitted Michaelis-Menten kinetics, which allowed us to give an estimation of the affinity of the fungal laccase for the different mediators. (C) 2002 Elsevier Science B.V. All rights reserved.
publishDate 2002
dc.date.none.fl_str_mv 2002
2002-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/1940
url http://hdl.handle.net/1822/1940
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv "Bioelectrochemistry". ISSN 1567-5394. 58 (2002) 149-156.
1567-5394
10.1016/S1567-5394(02)00119-6
12414320
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
_version_ 1799132592866328576

Registros relacionados