Validation study of the optical refractometry method to measure total proteins in calves’ serum
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo de conferência |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10174/33660 |
Resumo: | Failure of passive immune transfer is a condition attributed to calves that did not receive an appropriated doses of immunoglobulins through colostrum, which is known to compromise calf’s health and welfare, usually leading to an increase in antimicrobials’ usage. At the farm level, immunoglobulins analysis is not feasible, thus, the standard method consists in measuring the total protein concentration in serum (TSP) with an optical refractometer. The objective of this study was to evaluate the precision of the refractometer in measuring TSP. Samples of blood were collected from the jugular vein of 20 calves at birth and shortly after (day 1, 2 and 7). Blood samples were allowed to clot at room temperature for one hour and then refrigerated until centrifugation on the same day. After centrifugation, serum was obtained and frozen until the day of the analysis. TSP was measured with an optical refractometer (OR) and with an automated colorimetric method (Fujifilm DRI-CHEM; CM). No significant differences were found between both methods (P=0.203; OR – 5.8 ± 0.9 g/dL; CM – 5.9 ± 1.1 g/dL). TSP measured with both methods were highly correlated, specially at day 1 and 2 (r=0.74, 0.94, 0.96 and 0.83, at birth, day 1, 2 and 7, respectively). These results corroborate the use of the refractometer as a practical and economic screening tool to evaluate passive immune transfer at the farm level. |
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Validation study of the optical refractometry method to measure total proteins in calves’ serumcalvesDRI-CHEMoptical refractometerpassive immune transferFailure of passive immune transfer is a condition attributed to calves that did not receive an appropriated doses of immunoglobulins through colostrum, which is known to compromise calf’s health and welfare, usually leading to an increase in antimicrobials’ usage. At the farm level, immunoglobulins analysis is not feasible, thus, the standard method consists in measuring the total protein concentration in serum (TSP) with an optical refractometer. The objective of this study was to evaluate the precision of the refractometer in measuring TSP. Samples of blood were collected from the jugular vein of 20 calves at birth and shortly after (day 1, 2 and 7). Blood samples were allowed to clot at room temperature for one hour and then refrigerated until centrifugation on the same day. After centrifugation, serum was obtained and frozen until the day of the analysis. TSP was measured with an optical refractometer (OR) and with an automated colorimetric method (Fujifilm DRI-CHEM; CM). No significant differences were found between both methods (P=0.203; OR – 5.8 ± 0.9 g/dL; CM – 5.9 ± 1.1 g/dL). TSP measured with both methods were highly correlated, specially at day 1 and 2 (r=0.74, 0.94, 0.96 and 0.83, at birth, day 1, 2 and 7, respectively). These results corroborate the use of the refractometer as a practical and economic screening tool to evaluate passive immune transfer at the farm level.UTAD - Universidade de Trás-os-Montes e Alto Douro2023-01-25T16:41:58Z2023-01-252022-12-07T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjecthttp://hdl.handle.net/10174/33660http://hdl.handle.net/10174/33660engSilva, F.G., Martins, L., Azevedo, I., Pedro, S., Ramalho, J., Caetano, P., Cerqueira, J.L., Conceição, C., Silva, S.R., 2022. Validation study of the optical refractometry method to measure total proteins in calves’ serum. I International Congress on Animal and Veterinary Sciences “Under One Health Concept”, a 7 de dezembro de 2022. Livro de resumos, pág. 132.naonaosimfsilva@uevora.ptlmlm@uevora.ptndndjoana.ramalho94@gmail.comcaetano_90@hotmail.comcerqueira@esa.ipvc.ptcristinaconceicao@uevora.ptssilva@utad.ptSilva, FlávioMartins, LuísAzevedo, InêsPedro, SofiaRamalho, JoanaCaetano, PedroCerqueira, JoaquimConceição, CristinaSilva, Severianoinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-01-03T19:35:28Zoai:dspace.uevora.pt:10174/33660Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T01:22:21.551895Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Validation study of the optical refractometry method to measure total proteins in calves’ serum |
title |
Validation study of the optical refractometry method to measure total proteins in calves’ serum |
spellingShingle |
Validation study of the optical refractometry method to measure total proteins in calves’ serum Silva, Flávio calves DRI-CHEM optical refractometer passive immune transfer |
title_short |
Validation study of the optical refractometry method to measure total proteins in calves’ serum |
title_full |
Validation study of the optical refractometry method to measure total proteins in calves’ serum |
title_fullStr |
Validation study of the optical refractometry method to measure total proteins in calves’ serum |
title_full_unstemmed |
Validation study of the optical refractometry method to measure total proteins in calves’ serum |
title_sort |
Validation study of the optical refractometry method to measure total proteins in calves’ serum |
author |
Silva, Flávio |
author_facet |
Silva, Flávio Martins, Luís Azevedo, Inês Pedro, Sofia Ramalho, Joana Caetano, Pedro Cerqueira, Joaquim Conceição, Cristina Silva, Severiano |
author_role |
author |
author2 |
Martins, Luís Azevedo, Inês Pedro, Sofia Ramalho, Joana Caetano, Pedro Cerqueira, Joaquim Conceição, Cristina Silva, Severiano |
author2_role |
author author author author author author author author |
dc.contributor.author.fl_str_mv |
Silva, Flávio Martins, Luís Azevedo, Inês Pedro, Sofia Ramalho, Joana Caetano, Pedro Cerqueira, Joaquim Conceição, Cristina Silva, Severiano |
dc.subject.por.fl_str_mv |
calves DRI-CHEM optical refractometer passive immune transfer |
topic |
calves DRI-CHEM optical refractometer passive immune transfer |
description |
Failure of passive immune transfer is a condition attributed to calves that did not receive an appropriated doses of immunoglobulins through colostrum, which is known to compromise calf’s health and welfare, usually leading to an increase in antimicrobials’ usage. At the farm level, immunoglobulins analysis is not feasible, thus, the standard method consists in measuring the total protein concentration in serum (TSP) with an optical refractometer. The objective of this study was to evaluate the precision of the refractometer in measuring TSP. Samples of blood were collected from the jugular vein of 20 calves at birth and shortly after (day 1, 2 and 7). Blood samples were allowed to clot at room temperature for one hour and then refrigerated until centrifugation on the same day. After centrifugation, serum was obtained and frozen until the day of the analysis. TSP was measured with an optical refractometer (OR) and with an automated colorimetric method (Fujifilm DRI-CHEM; CM). No significant differences were found between both methods (P=0.203; OR – 5.8 ± 0.9 g/dL; CM – 5.9 ± 1.1 g/dL). TSP measured with both methods were highly correlated, specially at day 1 and 2 (r=0.74, 0.94, 0.96 and 0.83, at birth, day 1, 2 and 7, respectively). These results corroborate the use of the refractometer as a practical and economic screening tool to evaluate passive immune transfer at the farm level. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-12-07T00:00:00Z 2023-01-25T16:41:58Z 2023-01-25 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/conferenceObject |
format |
conferenceObject |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10174/33660 http://hdl.handle.net/10174/33660 |
url |
http://hdl.handle.net/10174/33660 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Silva, F.G., Martins, L., Azevedo, I., Pedro, S., Ramalho, J., Caetano, P., Cerqueira, J.L., Conceição, C., Silva, S.R., 2022. Validation study of the optical refractometry method to measure total proteins in calves’ serum. I International Congress on Animal and Veterinary Sciences “Under One Health Concept”, a 7 de dezembro de 2022. Livro de resumos, pág. 132. nao nao sim fsilva@uevora.pt lmlm@uevora.pt nd nd joana.ramalho94@gmail.com caetano_90@hotmail.com cerqueira@esa.ipvc.pt cristinaconceicao@uevora.pt ssilva@utad.pt |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
UTAD - Universidade de Trás-os-Montes e Alto Douro |
publisher.none.fl_str_mv |
UTAD - Universidade de Trás-os-Montes e Alto Douro |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1799136705517715456 |