Differential Expression of Syntaxin 1A and 1B by Noradrenergic and Adrenergic Chromaffin Cells
Autor(a) principal: | |
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Data de Publicação: | 2003 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10316/7905 https://doi.org/10.1023/A:1025658005937 |
Resumo: | The expression and localization of syntaxin isoforms 1A and 1B in adrenergic and noradrenergic chromaffin cells were examined by both immunoblot analysis and confocal immunofluorescence microscopy. Syntaxin 1A was found in higher levels in noradrenergic cells, whereas syntaxin 1B was similarly expressed in most noradrenergic and adrenergic cells. However, some heterogeneity was observed within each catecholaminergic phenotype. Although the majority of adrenergic cells appeared to express low levels of syntaxin 1A, about 7% was strongly stained for syntaxin 1A. A subpopulation of noradrenergic cells, about 17%, expressed greater levels of syntaxin 1B. Syntaxin 1B labeling showed a punctate appearance in the cytoplasm, whereas syntaxin 1A appeared predominantly localized to the plasma membrane. These data show differences in the exocytotic machinery of the two subtypes of chromaffin cells that may underlie some of the distinct characteristics of adrenaline and noradrenaline secretion. |
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Differential Expression of Syntaxin 1A and 1B by Noradrenergic and Adrenergic Chromaffin CellsThe expression and localization of syntaxin isoforms 1A and 1B in adrenergic and noradrenergic chromaffin cells were examined by both immunoblot analysis and confocal immunofluorescence microscopy. Syntaxin 1A was found in higher levels in noradrenergic cells, whereas syntaxin 1B was similarly expressed in most noradrenergic and adrenergic cells. However, some heterogeneity was observed within each catecholaminergic phenotype. Although the majority of adrenergic cells appeared to express low levels of syntaxin 1A, about 7% was strongly stained for syntaxin 1A. A subpopulation of noradrenergic cells, about 17%, expressed greater levels of syntaxin 1B. Syntaxin 1B labeling showed a punctate appearance in the cytoplasm, whereas syntaxin 1A appeared predominantly localized to the plasma membrane. These data show differences in the exocytotic machinery of the two subtypes of chromaffin cells that may underlie some of the distinct characteristics of adrenaline and noradrenaline secretion.2003info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://hdl.handle.net/10316/7905http://hdl.handle.net/10316/7905https://doi.org/10.1023/A:1025658005937engNeurochemical Research. 28:10 (2003) 1453-1457Baltazar, GraçaCarvalho, Arsélio P.Duarte, Emília P.info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2020-05-29T09:41:54Zoai:estudogeral.uc.pt:10316/7905Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T20:55:35.129100Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Differential Expression of Syntaxin 1A and 1B by Noradrenergic and Adrenergic Chromaffin Cells |
title |
Differential Expression of Syntaxin 1A and 1B by Noradrenergic and Adrenergic Chromaffin Cells |
spellingShingle |
Differential Expression of Syntaxin 1A and 1B by Noradrenergic and Adrenergic Chromaffin Cells Baltazar, Graça |
title_short |
Differential Expression of Syntaxin 1A and 1B by Noradrenergic and Adrenergic Chromaffin Cells |
title_full |
Differential Expression of Syntaxin 1A and 1B by Noradrenergic and Adrenergic Chromaffin Cells |
title_fullStr |
Differential Expression of Syntaxin 1A and 1B by Noradrenergic and Adrenergic Chromaffin Cells |
title_full_unstemmed |
Differential Expression of Syntaxin 1A and 1B by Noradrenergic and Adrenergic Chromaffin Cells |
title_sort |
Differential Expression of Syntaxin 1A and 1B by Noradrenergic and Adrenergic Chromaffin Cells |
author |
Baltazar, Graça |
author_facet |
Baltazar, Graça Carvalho, Arsélio P. Duarte, Emília P. |
author_role |
author |
author2 |
Carvalho, Arsélio P. Duarte, Emília P. |
author2_role |
author author |
dc.contributor.author.fl_str_mv |
Baltazar, Graça Carvalho, Arsélio P. Duarte, Emília P. |
description |
The expression and localization of syntaxin isoforms 1A and 1B in adrenergic and noradrenergic chromaffin cells were examined by both immunoblot analysis and confocal immunofluorescence microscopy. Syntaxin 1A was found in higher levels in noradrenergic cells, whereas syntaxin 1B was similarly expressed in most noradrenergic and adrenergic cells. However, some heterogeneity was observed within each catecholaminergic phenotype. Although the majority of adrenergic cells appeared to express low levels of syntaxin 1A, about 7% was strongly stained for syntaxin 1A. A subpopulation of noradrenergic cells, about 17%, expressed greater levels of syntaxin 1B. Syntaxin 1B labeling showed a punctate appearance in the cytoplasm, whereas syntaxin 1A appeared predominantly localized to the plasma membrane. These data show differences in the exocytotic machinery of the two subtypes of chromaffin cells that may underlie some of the distinct characteristics of adrenaline and noradrenaline secretion. |
publishDate |
2003 |
dc.date.none.fl_str_mv |
2003 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10316/7905 http://hdl.handle.net/10316/7905 https://doi.org/10.1023/A:1025658005937 |
url |
http://hdl.handle.net/10316/7905 https://doi.org/10.1023/A:1025658005937 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Neurochemical Research. 28:10 (2003) 1453-1457 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
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RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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