A simplified protocol for Agrobacterium-mediated transformation of cell suspension cultures of the model species Medicago truncatula A17

Detalhes bibliográficos
Autor(a) principal: Ferreira, Ana Clara
Data de Publicação: 2023
Outros Autores: Rebelo, Bárbara A., Abranches, Rita
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/158481
Resumo: Funding Information: Open access funding provided by FCT|FCCN (b-on). This work was supported by Fundação para a Ciência e Tecnologia (FCT, Portugal) through Research Units “Molecular, Structural and Cellular Microbiology” (MOSTMICRO, UIDB/ 04612/2020) and “Bioresources 4 Sustainability” (GREEN-IT, UIDB/04551/2020). Publisher Copyright: © 2023, The Author(s).
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spelling A simplified protocol for Agrobacterium-mediated transformation of cell suspension cultures of the model species Medicago truncatula A17AgrobacteriumCultured cellsMedicagoModel legumePlant-based systemRBDSARS-CoV-2Stable transformationHorticultureFunding Information: Open access funding provided by FCT|FCCN (b-on). This work was supported by Fundação para a Ciência e Tecnologia (FCT, Portugal) through Research Units “Molecular, Structural and Cellular Microbiology” (MOSTMICRO, UIDB/ 04612/2020) and “Bioresources 4 Sustainability” (GREEN-IT, UIDB/04551/2020). Publisher Copyright: © 2023, The Author(s).This manuscript describes a unique protocol for the rapid transformation of Medicago truncatula A17 cell suspension cultures mediated by Agrobacterium tumefaciens. Medicago cells were collected on day 7 of the growth curve, which corresponded to the beginning of the exponential phase. They were then co-cultured with Agrobacterium for 3 days before being spread onto a petri dish with appropriate antibiotic selection. The Receptor Binding Domain of the Spike protein of SARS-CoV-2 was used as a model to develop this protocol. The presence of the transgene was assessed using PCR, and the integrity of the product was evaluated by SDS-PAGE and Western-blotting.Instituto de Tecnologia Química e Biológica António Xavier (ITQB)RUNFerreira, Ana ClaraRebelo, Bárbara A.Abranches, Rita2023-09-29T22:22:09Z2023-062023-06-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article7application/pdfhttp://hdl.handle.net/10362/158481eng0167-6857PURE: 72725949https://doi.org/10.1007/s11240-023-02495-6info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T05:40:53Zoai:run.unl.pt:10362/158481Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:57:09.831964Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv A simplified protocol for Agrobacterium-mediated transformation of cell suspension cultures of the model species Medicago truncatula A17
title A simplified protocol for Agrobacterium-mediated transformation of cell suspension cultures of the model species Medicago truncatula A17
spellingShingle A simplified protocol for Agrobacterium-mediated transformation of cell suspension cultures of the model species Medicago truncatula A17
Ferreira, Ana Clara
Agrobacterium
Cultured cells
Medicago
Model legume
Plant-based system
RBD
SARS-CoV-2
Stable transformation
Horticulture
title_short A simplified protocol for Agrobacterium-mediated transformation of cell suspension cultures of the model species Medicago truncatula A17
title_full A simplified protocol for Agrobacterium-mediated transformation of cell suspension cultures of the model species Medicago truncatula A17
title_fullStr A simplified protocol for Agrobacterium-mediated transformation of cell suspension cultures of the model species Medicago truncatula A17
title_full_unstemmed A simplified protocol for Agrobacterium-mediated transformation of cell suspension cultures of the model species Medicago truncatula A17
title_sort A simplified protocol for Agrobacterium-mediated transformation of cell suspension cultures of the model species Medicago truncatula A17
author Ferreira, Ana Clara
author_facet Ferreira, Ana Clara
Rebelo, Bárbara A.
Abranches, Rita
author_role author
author2 Rebelo, Bárbara A.
Abranches, Rita
author2_role author
author
dc.contributor.none.fl_str_mv Instituto de Tecnologia Química e Biológica António Xavier (ITQB)
RUN
dc.contributor.author.fl_str_mv Ferreira, Ana Clara
Rebelo, Bárbara A.
Abranches, Rita
dc.subject.por.fl_str_mv Agrobacterium
Cultured cells
Medicago
Model legume
Plant-based system
RBD
SARS-CoV-2
Stable transformation
Horticulture
topic Agrobacterium
Cultured cells
Medicago
Model legume
Plant-based system
RBD
SARS-CoV-2
Stable transformation
Horticulture
description Funding Information: Open access funding provided by FCT|FCCN (b-on). This work was supported by Fundação para a Ciência e Tecnologia (FCT, Portugal) through Research Units “Molecular, Structural and Cellular Microbiology” (MOSTMICRO, UIDB/ 04612/2020) and “Bioresources 4 Sustainability” (GREEN-IT, UIDB/04551/2020). Publisher Copyright: © 2023, The Author(s).
publishDate 2023
dc.date.none.fl_str_mv 2023-09-29T22:22:09Z
2023-06
2023-06-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/158481
url http://hdl.handle.net/10362/158481
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 0167-6857
PURE: 72725949
https://doi.org/10.1007/s11240-023-02495-6
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 7
application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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