Berberine reverts hepatic mitochondrial dysfunction in high-fat fed rats: A possible role for SirT3 activation
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10316/25615 https://doi.org/10.1016/j.mito.2013.09.002 |
Resumo: | Berberine is an isoquinoline alkaloid with anti-diabetic properties. Despite the central role of liver and thus hepaticmitochondria inwhole-bodymetabolism, berberine effects on hepaticmitochondrial function in an obesity model are still unknown. Here, we demonstrate that berberine treatment recovers mitochondrial efficiency when altered by a high-fat feeding.Mitochondria isolated from the liver of high-fat fed rats exhibited decreased capacity to accumulate calcium and impaired oxidative phosphorylation (OXPHOS) capacity, as shown by impaired mitochondrialmembrane potential, oxygen consumption and cellular ATP levels. Interestingly, the recovery of mitochondrial function by berberine was associated with an increased activity of the mitochondrial sirtuin 3 (SirT3). In conclusion, berberine potent protective effects against metabolic syndrome may rely on increasing mitochondrial SirT3 activity, normalizing mitochondrial function and preventing a state of energetic deficit caused by impaired OXPHOS. |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Berberine reverts hepatic mitochondrial dysfunction in high-fat fed rats: A possible role for SirT3 activationBerberineMitochondrial functionLiver bioenergeticsObesitySirT3Berberine is an isoquinoline alkaloid with anti-diabetic properties. Despite the central role of liver and thus hepaticmitochondria inwhole-bodymetabolism, berberine effects on hepaticmitochondrial function in an obesity model are still unknown. Here, we demonstrate that berberine treatment recovers mitochondrial efficiency when altered by a high-fat feeding.Mitochondria isolated from the liver of high-fat fed rats exhibited decreased capacity to accumulate calcium and impaired oxidative phosphorylation (OXPHOS) capacity, as shown by impaired mitochondrialmembrane potential, oxygen consumption and cellular ATP levels. Interestingly, the recovery of mitochondrial function by berberine was associated with an increased activity of the mitochondrial sirtuin 3 (SirT3). In conclusion, berberine potent protective effects against metabolic syndrome may rely on increasing mitochondrial SirT3 activity, normalizing mitochondrial function and preventing a state of energetic deficit caused by impaired OXPHOS.JST, FVD, APG and ATV were recipients of a Fundação para a Ciência e a Tecnologia PhD scholarship (SFRH/BD/38467/2007, SFRH/BD/38372/ 2007, SFRH/BD/44674/2008 and SFRH/BD/44796/2008, respectively). This project was supported by a FCT grant PTCD/SAU-OSM/72443/ 2006.Elsevier B.V.2013info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://hdl.handle.net/10316/25615http://hdl.handle.net/10316/25615https://doi.org/10.1016/j.mito.2013.09.002enghttp://www.sciencedirect.com/science/article/pii/S1567724913002468#Teodoro, João SoeiroDuarte, Filipe ValenteGomes, Ana PatríciaVarela, Ana TeresaPeixoto, Francisco ManuelRolo, Anabela PintoPalmeira, C. M.info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2021-11-04T09:20:15Zoai:estudogeral.uc.pt:10316/25615Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T20:56:02.783551Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Berberine reverts hepatic mitochondrial dysfunction in high-fat fed rats: A possible role for SirT3 activation |
title |
Berberine reverts hepatic mitochondrial dysfunction in high-fat fed rats: A possible role for SirT3 activation |
spellingShingle |
Berberine reverts hepatic mitochondrial dysfunction in high-fat fed rats: A possible role for SirT3 activation Teodoro, João Soeiro Berberine Mitochondrial function Liver bioenergetics Obesity SirT3 |
title_short |
Berberine reverts hepatic mitochondrial dysfunction in high-fat fed rats: A possible role for SirT3 activation |
title_full |
Berberine reverts hepatic mitochondrial dysfunction in high-fat fed rats: A possible role for SirT3 activation |
title_fullStr |
Berberine reverts hepatic mitochondrial dysfunction in high-fat fed rats: A possible role for SirT3 activation |
title_full_unstemmed |
Berberine reverts hepatic mitochondrial dysfunction in high-fat fed rats: A possible role for SirT3 activation |
title_sort |
Berberine reverts hepatic mitochondrial dysfunction in high-fat fed rats: A possible role for SirT3 activation |
author |
Teodoro, João Soeiro |
author_facet |
Teodoro, João Soeiro Duarte, Filipe Valente Gomes, Ana Patrícia Varela, Ana Teresa Peixoto, Francisco Manuel Rolo, Anabela Pinto Palmeira, C. M. |
author_role |
author |
author2 |
Duarte, Filipe Valente Gomes, Ana Patrícia Varela, Ana Teresa Peixoto, Francisco Manuel Rolo, Anabela Pinto Palmeira, C. M. |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Teodoro, João Soeiro Duarte, Filipe Valente Gomes, Ana Patrícia Varela, Ana Teresa Peixoto, Francisco Manuel Rolo, Anabela Pinto Palmeira, C. M. |
dc.subject.por.fl_str_mv |
Berberine Mitochondrial function Liver bioenergetics Obesity SirT3 |
topic |
Berberine Mitochondrial function Liver bioenergetics Obesity SirT3 |
description |
Berberine is an isoquinoline alkaloid with anti-diabetic properties. Despite the central role of liver and thus hepaticmitochondria inwhole-bodymetabolism, berberine effects on hepaticmitochondrial function in an obesity model are still unknown. Here, we demonstrate that berberine treatment recovers mitochondrial efficiency when altered by a high-fat feeding.Mitochondria isolated from the liver of high-fat fed rats exhibited decreased capacity to accumulate calcium and impaired oxidative phosphorylation (OXPHOS) capacity, as shown by impaired mitochondrialmembrane potential, oxygen consumption and cellular ATP levels. Interestingly, the recovery of mitochondrial function by berberine was associated with an increased activity of the mitochondrial sirtuin 3 (SirT3). In conclusion, berberine potent protective effects against metabolic syndrome may rely on increasing mitochondrial SirT3 activity, normalizing mitochondrial function and preventing a state of energetic deficit caused by impaired OXPHOS. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10316/25615 http://hdl.handle.net/10316/25615 https://doi.org/10.1016/j.mito.2013.09.002 |
url |
http://hdl.handle.net/10316/25615 https://doi.org/10.1016/j.mito.2013.09.002 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
http://www.sciencedirect.com/science/article/pii/S1567724913002468# |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
Elsevier B.V. |
publisher.none.fl_str_mv |
Elsevier B.V. |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
|
_version_ |
1799133845596930048 |