Enzymatic phosphorylation of hair keratin enhances fast adsorption of cationic moieties

Detalhes bibliográficos
Autor(a) principal: Volkov, Vadim
Data de Publicação: 2016
Outros Autores: Cavaco-Paulo, Artur
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/40192
Resumo: The current study describes the in vitro phosphorylation of a human hair keratin, using protein kinase for the first time. Phosphorylation of keratin was demonstrated by 31P NMR (Nuclear Magnetic Resonance) and Diffuse Reflectance Infrared Fourier Transform (DRIFT) techniques. Phosphorylation induced a 2.5 fold increase of adsorption capacity in the first 10 minutes for cationic moiety like Methylene Blue (MB). Thorough description of MB adsorption process was performed by several isothermal models. Reconstructed fluorescent microscopy images depict distinct amounts of dye bound to the differently treated hair. The results of this work suggest that the enzymatic phosphorylation of keratins might have significant implications in hair shampooing and conditioning, where short application times of cationic components are of prime importance.
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spelling Enzymatic phosphorylation of hair keratin enhances fast adsorption of cationic moietiesHair keratinMethylene bluePhosphorylationScience & TechnologyThe current study describes the in vitro phosphorylation of a human hair keratin, using protein kinase for the first time. Phosphorylation of keratin was demonstrated by 31P NMR (Nuclear Magnetic Resonance) and Diffuse Reflectance Infrared Fourier Transform (DRIFT) techniques. Phosphorylation induced a 2.5 fold increase of adsorption capacity in the first 10 minutes for cationic moiety like Methylene Blue (MB). Thorough description of MB adsorption process was performed by several isothermal models. Reconstructed fluorescent microscopy images depict distinct amounts of dye bound to the differently treated hair. The results of this work suggest that the enzymatic phosphorylation of keratins might have significant implications in hair shampooing and conditioning, where short application times of cationic components are of prime importance.The authors would like to acknowledge the support, granted by the following projects: “BioEnv”—Biotechnology and Bioengineering for a sustainable world”, REF. NORTE-07-0124-FEDER-000048; and “BioInd”—Biotechnology and Bioengineering for improved Industrial and Agro-Food processes”, REF. NORTE-07-0124-FEDER- 000028. Both projects are co-funded by the Programa Operacional Regional do Norte (ON.2–O Novo Norte), QREN, FEDER.Elsevier B.V.Universidade do MinhoVolkov, VadimCavaco-Paulo, Artur2016-042016-04-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/40192engVolkov, V.; Paulo, Artur Cavaco, Enzymatic phosphorylation of hair keratin enhances fast adsorption of cationic moieties. International Journal of Biological Macromolecules, 85, 476-486, 20160141-813010.1016/j.ijbiomac.2015.12.08226756110http://www.elsevier.com/locate/issn/01418130info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:29:22ZPortal AgregadorONG
dc.title.none.fl_str_mv Enzymatic phosphorylation of hair keratin enhances fast adsorption of cationic moieties
title Enzymatic phosphorylation of hair keratin enhances fast adsorption of cationic moieties
spellingShingle Enzymatic phosphorylation of hair keratin enhances fast adsorption of cationic moieties
Volkov, Vadim
Hair keratin
Methylene blue
Phosphorylation
Science & Technology
title_short Enzymatic phosphorylation of hair keratin enhances fast adsorption of cationic moieties
title_full Enzymatic phosphorylation of hair keratin enhances fast adsorption of cationic moieties
title_fullStr Enzymatic phosphorylation of hair keratin enhances fast adsorption of cationic moieties
title_full_unstemmed Enzymatic phosphorylation of hair keratin enhances fast adsorption of cationic moieties
title_sort Enzymatic phosphorylation of hair keratin enhances fast adsorption of cationic moieties
author Volkov, Vadim
author_facet Volkov, Vadim
Cavaco-Paulo, Artur
author_role author
author2 Cavaco-Paulo, Artur
author2_role author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Volkov, Vadim
Cavaco-Paulo, Artur
dc.subject.por.fl_str_mv Hair keratin
Methylene blue
Phosphorylation
Science & Technology
topic Hair keratin
Methylene blue
Phosphorylation
Science & Technology
description The current study describes the in vitro phosphorylation of a human hair keratin, using protein kinase for the first time. Phosphorylation of keratin was demonstrated by 31P NMR (Nuclear Magnetic Resonance) and Diffuse Reflectance Infrared Fourier Transform (DRIFT) techniques. Phosphorylation induced a 2.5 fold increase of adsorption capacity in the first 10 minutes for cationic moiety like Methylene Blue (MB). Thorough description of MB adsorption process was performed by several isothermal models. Reconstructed fluorescent microscopy images depict distinct amounts of dye bound to the differently treated hair. The results of this work suggest that the enzymatic phosphorylation of keratins might have significant implications in hair shampooing and conditioning, where short application times of cationic components are of prime importance.
publishDate 2016
dc.date.none.fl_str_mv 2016-04
2016-04-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/40192
url http://hdl.handle.net/1822/40192
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Volkov, V.; Paulo, Artur Cavaco, Enzymatic phosphorylation of hair keratin enhances fast adsorption of cationic moieties. International Journal of Biological Macromolecules, 85, 476-486, 2016
0141-8130
10.1016/j.ijbiomac.2015.12.082
26756110
http://www.elsevier.com/locate/issn/01418130
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier B.V.
publisher.none.fl_str_mv Elsevier B.V.
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
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repository.mail.fl_str_mv
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