Enzymatic phosphorylation of hair keratin enhances fast adsorption of cationic moieties
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Publication Date: | 2016 |
Other Authors: | |
Format: | Article |
Language: | eng |
Source: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Download full: | http://hdl.handle.net/1822/40192 |
Summary: | The current study describes the in vitro phosphorylation of a human hair keratin, using protein kinase for the first time. Phosphorylation of keratin was demonstrated by 31P NMR (Nuclear Magnetic Resonance) and Diffuse Reflectance Infrared Fourier Transform (DRIFT) techniques. Phosphorylation induced a 2.5 fold increase of adsorption capacity in the first 10 minutes for cationic moiety like Methylene Blue (MB). Thorough description of MB adsorption process was performed by several isothermal models. Reconstructed fluorescent microscopy images depict distinct amounts of dye bound to the differently treated hair. The results of this work suggest that the enzymatic phosphorylation of keratins might have significant implications in hair shampooing and conditioning, where short application times of cationic components are of prime importance. |
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Enzymatic phosphorylation of hair keratin enhances fast adsorption of cationic moietiesHair keratinMethylene bluePhosphorylationScience & TechnologyThe current study describes the in vitro phosphorylation of a human hair keratin, using protein kinase for the first time. Phosphorylation of keratin was demonstrated by 31P NMR (Nuclear Magnetic Resonance) and Diffuse Reflectance Infrared Fourier Transform (DRIFT) techniques. Phosphorylation induced a 2.5 fold increase of adsorption capacity in the first 10 minutes for cationic moiety like Methylene Blue (MB). Thorough description of MB adsorption process was performed by several isothermal models. Reconstructed fluorescent microscopy images depict distinct amounts of dye bound to the differently treated hair. The results of this work suggest that the enzymatic phosphorylation of keratins might have significant implications in hair shampooing and conditioning, where short application times of cationic components are of prime importance.The authors would like to acknowledge the support, granted by the following projects: “BioEnv”—Biotechnology and Bioengineering for a sustainable world”, REF. NORTE-07-0124-FEDER-000048; and “BioInd”—Biotechnology and Bioengineering for improved Industrial and Agro-Food processes”, REF. NORTE-07-0124-FEDER- 000028. Both projects are co-funded by the Programa Operacional Regional do Norte (ON.2–O Novo Norte), QREN, FEDER.Elsevier B.V.Universidade do MinhoVolkov, VadimCavaco-Paulo, Artur2016-042016-04-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/40192engVolkov, V.; Paulo, Artur Cavaco, Enzymatic phosphorylation of hair keratin enhances fast adsorption of cationic moieties. International Journal of Biological Macromolecules, 85, 476-486, 20160141-813010.1016/j.ijbiomac.2015.12.08226756110http://www.elsevier.com/locate/issn/01418130info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:29:22Zoai:repositorium.sdum.uminho.pt:1822/40192Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:24:20.957063Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Enzymatic phosphorylation of hair keratin enhances fast adsorption of cationic moieties |
title |
Enzymatic phosphorylation of hair keratin enhances fast adsorption of cationic moieties |
spellingShingle |
Enzymatic phosphorylation of hair keratin enhances fast adsorption of cationic moieties Volkov, Vadim Hair keratin Methylene blue Phosphorylation Science & Technology |
title_short |
Enzymatic phosphorylation of hair keratin enhances fast adsorption of cationic moieties |
title_full |
Enzymatic phosphorylation of hair keratin enhances fast adsorption of cationic moieties |
title_fullStr |
Enzymatic phosphorylation of hair keratin enhances fast adsorption of cationic moieties |
title_full_unstemmed |
Enzymatic phosphorylation of hair keratin enhances fast adsorption of cationic moieties |
title_sort |
Enzymatic phosphorylation of hair keratin enhances fast adsorption of cationic moieties |
author |
Volkov, Vadim |
author_facet |
Volkov, Vadim Cavaco-Paulo, Artur |
author_role |
author |
author2 |
Cavaco-Paulo, Artur |
author2_role |
author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Volkov, Vadim Cavaco-Paulo, Artur |
dc.subject.por.fl_str_mv |
Hair keratin Methylene blue Phosphorylation Science & Technology |
topic |
Hair keratin Methylene blue Phosphorylation Science & Technology |
description |
The current study describes the in vitro phosphorylation of a human hair keratin, using protein kinase for the first time. Phosphorylation of keratin was demonstrated by 31P NMR (Nuclear Magnetic Resonance) and Diffuse Reflectance Infrared Fourier Transform (DRIFT) techniques. Phosphorylation induced a 2.5 fold increase of adsorption capacity in the first 10 minutes for cationic moiety like Methylene Blue (MB). Thorough description of MB adsorption process was performed by several isothermal models. Reconstructed fluorescent microscopy images depict distinct amounts of dye bound to the differently treated hair. The results of this work suggest that the enzymatic phosphorylation of keratins might have significant implications in hair shampooing and conditioning, where short application times of cationic components are of prime importance. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-04 2016-04-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/40192 |
url |
http://hdl.handle.net/1822/40192 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Volkov, V.; Paulo, Artur Cavaco, Enzymatic phosphorylation of hair keratin enhances fast adsorption of cationic moieties. International Journal of Biological Macromolecules, 85, 476-486, 2016 0141-8130 10.1016/j.ijbiomac.2015.12.082 26756110 http://www.elsevier.com/locate/issn/01418130 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier B.V. |
publisher.none.fl_str_mv |
Elsevier B.V. |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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