Contribution of Yap 1 towards S. cervisiae adaptation to arsenic mediated oxidative stress

Detalhes bibliográficos
Autor(a) principal: Menezes, Regina A.
Data de Publicação: 2008
Outros Autores: Amaral, Catarina, Batista-Nascimento, Liliana, Santos, Cláudia, Ferreira, Ricardo Boavida, Devaux, Fréderic, Eleutherio, Elis C.A., Rodrigues-Pousada, Claudina
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.5/5599
Resumo: Post-Print
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spelling Contribution of Yap 1 towards S. cervisiae adaptation to arsenic mediated oxidative stressarsenic stressoxidative stressYAP 1YAP 8 (ACR 1; ARR 1)transcriptional regulationPost-PrintIn the budding yeast Saccharomyces cerevisiae arsenic detoxification involves the activation Yap8, a member of the Yap family of transcription factors, which in turn regulates ACR2 and ACR3, encoding an arsenate reductase and a plasma membrane arsenite efflux-protein, respectively. In addition, Yap1 is involved in the arsenic adaptation process through regulating the expression of the vacuolar-pump encoded by YCF1 and also contributing to the regulation of ACR genes. Here we show that Yap1 is also involved in the removal of ROS generated by arsenic compounds. Data on lipid peroxidation and intracellular oxidation indicate that deletion of YAP1 and YAP8 triggers cellular oxidation mediated by inorganic arsenic. In spite of the increased amounts of As(III) absorbed by the yap8 mutant, the enhanced transcriptional activation of the antioxidant genes such as GSH1, SOD1 and TRX2 may prevent protein oxidation. In contrast, the yap1 mutant exhibits high contents of protein carbonyl groups and the GSSG:GSH ratio is severely disturbed upon exposure to arsenic compounds in these cells. These results point to an additional level of Yap1 contribution to arsenic stress responses by preventing oxidative damage in cells exposed to these compounds. Transcriptional profiling revealed that genes of the functional categories related with sulphur and methionine metabolism and with the maintenance of the cell redox homeostasis are activated to mediate adaptation of the wild type strain to 2 mM arsenate treatmentBiochemical SocietyRepositório da Universidade de LisboaMenezes, Regina A.Amaral, CatarinaBatista-Nascimento, LilianaSantos, CláudiaFerreira, Ricardo BoavidaDevaux, FrédericEleutherio, Elis C.A.Rodrigues-Pousada, Claudina2013-05-30T16:04:05Z20082008-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.5/5599enginfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-03-06T14:36:28Zoai:www.repository.utl.pt:10400.5/5599Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T16:53:04.335222Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Contribution of Yap 1 towards S. cervisiae adaptation to arsenic mediated oxidative stress
title Contribution of Yap 1 towards S. cervisiae adaptation to arsenic mediated oxidative stress
spellingShingle Contribution of Yap 1 towards S. cervisiae adaptation to arsenic mediated oxidative stress
Menezes, Regina A.
arsenic stress
oxidative stress
YAP 1
YAP 8 (ACR 1; ARR 1)
transcriptional regulation
title_short Contribution of Yap 1 towards S. cervisiae adaptation to arsenic mediated oxidative stress
title_full Contribution of Yap 1 towards S. cervisiae adaptation to arsenic mediated oxidative stress
title_fullStr Contribution of Yap 1 towards S. cervisiae adaptation to arsenic mediated oxidative stress
title_full_unstemmed Contribution of Yap 1 towards S. cervisiae adaptation to arsenic mediated oxidative stress
title_sort Contribution of Yap 1 towards S. cervisiae adaptation to arsenic mediated oxidative stress
author Menezes, Regina A.
author_facet Menezes, Regina A.
Amaral, Catarina
Batista-Nascimento, Liliana
Santos, Cláudia
Ferreira, Ricardo Boavida
Devaux, Fréderic
Eleutherio, Elis C.A.
Rodrigues-Pousada, Claudina
author_role author
author2 Amaral, Catarina
Batista-Nascimento, Liliana
Santos, Cláudia
Ferreira, Ricardo Boavida
Devaux, Fréderic
Eleutherio, Elis C.A.
Rodrigues-Pousada, Claudina
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Menezes, Regina A.
Amaral, Catarina
Batista-Nascimento, Liliana
Santos, Cláudia
Ferreira, Ricardo Boavida
Devaux, Fréderic
Eleutherio, Elis C.A.
Rodrigues-Pousada, Claudina
dc.subject.por.fl_str_mv arsenic stress
oxidative stress
YAP 1
YAP 8 (ACR 1; ARR 1)
transcriptional regulation
topic arsenic stress
oxidative stress
YAP 1
YAP 8 (ACR 1; ARR 1)
transcriptional regulation
description Post-Print
publishDate 2008
dc.date.none.fl_str_mv 2008
2008-01-01T00:00:00Z
2013-05-30T16:04:05Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.5/5599
url http://hdl.handle.net/10400.5/5599
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Biochemical Society
publisher.none.fl_str_mv Biochemical Society
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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