Controlled drug delivery for surface protection of prostheses
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Tipo de documento: | Dissertação |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10362/153475 |
Resumo: | This work aimed to study the application of a multilayer polymeric coating on stainless-steel (SS) sur- faces to provide local and controlled drug delivery to prevent the formation of biofilms. The bacteria colonies formed onto non-recognized surfaces placed inside the body could cause serious infections leading to the im- plant’s removal. Therefore, implant’s surface functionalization is a key point to overcome this problem. Using electrospinning and blow-spinning techniques, the SS substrates were coated layer-by-layer with membranes made of cellulose acetate (CA) and polycaprolactone (PCL) fibres containing ibuprofen (Ibu). The fibres retain the mechanical properties of the polymers while also allow for controlled Ibu release in specific locations. As a result, Ibu efficacy improves and the ability of implant rejection decreases. Optical microscopy, scanning electron microscopy, and Raman spectroscopy were used to characterize the fibres. Following the films production, drug release, swelling, and degradation tests were carried out to simu- late in vivo performance of the membranes. Mechanical properties were also evaluated through stress and peeling-off adhesion tests. A layer of chitosan (CHI) was electrosprayed between the substrate and the coating for the peeling-off tests, to evaluate the effect of CHI on adhesion. Ibu was successfully incorporated into the fibres, but the concentrations and control of its release require further investigation. The electrosprayed CHI layer improved the adhesion of the coating to the surfaces. In conclusion, this research has demonstrated that the films obtained have promising properties for use in the context of implant-related infection treatment. |
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Controlled drug delivery for surface protection of prosthesesCellulose acetatepolycaprolactoneibuprofenelectrospinningblow-spinningdrug releaseDomínio/Área Científica::Engenharia e Tecnologia::NanotecnologiaThis work aimed to study the application of a multilayer polymeric coating on stainless-steel (SS) sur- faces to provide local and controlled drug delivery to prevent the formation of biofilms. The bacteria colonies formed onto non-recognized surfaces placed inside the body could cause serious infections leading to the im- plant’s removal. Therefore, implant’s surface functionalization is a key point to overcome this problem. Using electrospinning and blow-spinning techniques, the SS substrates were coated layer-by-layer with membranes made of cellulose acetate (CA) and polycaprolactone (PCL) fibres containing ibuprofen (Ibu). The fibres retain the mechanical properties of the polymers while also allow for controlled Ibu release in specific locations. As a result, Ibu efficacy improves and the ability of implant rejection decreases. Optical microscopy, scanning electron microscopy, and Raman spectroscopy were used to characterize the fibres. Following the films production, drug release, swelling, and degradation tests were carried out to simu- late in vivo performance of the membranes. Mechanical properties were also evaluated through stress and peeling-off adhesion tests. A layer of chitosan (CHI) was electrosprayed between the substrate and the coating for the peeling-off tests, to evaluate the effect of CHI on adhesion. Ibu was successfully incorporated into the fibres, but the concentrations and control of its release require further investigation. The electrosprayed CHI layer improved the adhesion of the coating to the surfaces. In conclusion, this research has demonstrated that the films obtained have promising properties for use in the context of implant-related infection treatment.Este trabalho teve como objetivo estudar a aplicação de um revestimento polimérico multicamada em superfícies de aço inoxidável (SS) para fornecer libertação local e controlada de fármacos para prevenir a formação de biofilmes. As colónias de bactérias formadas em superfícies não reconhecidas colocadas dentro do corpo podem causar infeções graves que levam à remoção do implante. Portanto, a funcionalização da superfície do implante é um ponto chave para superar este problema. Utilizando técnicas de eletrofiação e blow-spinning, os substratos SS foram revestidos camada por ca- mada com membranas feitas de fibras de acetato de celulose (CA) e de policaprolactona (PCL) incorporando ibuprofeno (Ibu). As fibras retêm as propriedades mecânicas dos polímeros, permitindo também a libertação controlada de Ibu em locais específicos. Como resultado, a eficácia do Ibu melhora e a capacidade de rejeição do implante diminui. Microscopia ótica, microscopia eletrónica de varredura e espectroscopia Raman foram utilizadas para caracterizar as fibras. Após a produção dos filmes, testes de libertação do fármaco, inchamento e degradação foram realizados para simular o desempenho in vivo das membranas. As propriedades mecânicas também foram avaliadas por meio de testes de tração e adesão. Uma camada de quitosana (CHI) foi eletropulverizada entre o substrato e o revestimento para avaliar o efeito da CHI nos testes de adesão. O Ibu foi incorporado com sucesso nas fibras, mas as concentrações e o controlo da sua libertação re- querem investigação adicional. A camada de CHI eletropulverizada melhorou a adesão do revestimento às superfícies. Concluindo, esta pesquisa demonstrou que os filmes obtidos possuem propriedades promissoras para uso no contexto do tratamento de infeções relacionadas a implantes.Ferreira, IsabelBaptista, AnaRUNCarmo, Catarina Paulino Cidade do2023-06-02T13:32:58Z2022-112022-11-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfhttp://hdl.handle.net/10362/153475enginfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T05:35:58Zoai:run.unl.pt:10362/153475Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:55:17.005862Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Controlled drug delivery for surface protection of prostheses |
title |
Controlled drug delivery for surface protection of prostheses |
spellingShingle |
Controlled drug delivery for surface protection of prostheses Carmo, Catarina Paulino Cidade do Cellulose acetate polycaprolactone ibuprofen electrospinning blow-spinning drug release Domínio/Área Científica::Engenharia e Tecnologia::Nanotecnologia |
title_short |
Controlled drug delivery for surface protection of prostheses |
title_full |
Controlled drug delivery for surface protection of prostheses |
title_fullStr |
Controlled drug delivery for surface protection of prostheses |
title_full_unstemmed |
Controlled drug delivery for surface protection of prostheses |
title_sort |
Controlled drug delivery for surface protection of prostheses |
author |
Carmo, Catarina Paulino Cidade do |
author_facet |
Carmo, Catarina Paulino Cidade do |
author_role |
author |
dc.contributor.none.fl_str_mv |
Ferreira, Isabel Baptista, Ana RUN |
dc.contributor.author.fl_str_mv |
Carmo, Catarina Paulino Cidade do |
dc.subject.por.fl_str_mv |
Cellulose acetate polycaprolactone ibuprofen electrospinning blow-spinning drug release Domínio/Área Científica::Engenharia e Tecnologia::Nanotecnologia |
topic |
Cellulose acetate polycaprolactone ibuprofen electrospinning blow-spinning drug release Domínio/Área Científica::Engenharia e Tecnologia::Nanotecnologia |
description |
This work aimed to study the application of a multilayer polymeric coating on stainless-steel (SS) sur- faces to provide local and controlled drug delivery to prevent the formation of biofilms. The bacteria colonies formed onto non-recognized surfaces placed inside the body could cause serious infections leading to the im- plant’s removal. Therefore, implant’s surface functionalization is a key point to overcome this problem. Using electrospinning and blow-spinning techniques, the SS substrates were coated layer-by-layer with membranes made of cellulose acetate (CA) and polycaprolactone (PCL) fibres containing ibuprofen (Ibu). The fibres retain the mechanical properties of the polymers while also allow for controlled Ibu release in specific locations. As a result, Ibu efficacy improves and the ability of implant rejection decreases. Optical microscopy, scanning electron microscopy, and Raman spectroscopy were used to characterize the fibres. Following the films production, drug release, swelling, and degradation tests were carried out to simu- late in vivo performance of the membranes. Mechanical properties were also evaluated through stress and peeling-off adhesion tests. A layer of chitosan (CHI) was electrosprayed between the substrate and the coating for the peeling-off tests, to evaluate the effect of CHI on adhesion. Ibu was successfully incorporated into the fibres, but the concentrations and control of its release require further investigation. The electrosprayed CHI layer improved the adhesion of the coating to the surfaces. In conclusion, this research has demonstrated that the films obtained have promising properties for use in the context of implant-related infection treatment. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-11 2022-11-01T00:00:00Z 2023-06-02T13:32:58Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10362/153475 |
url |
http://hdl.handle.net/10362/153475 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799138140169961472 |