Species-specific RNA-FISH probes for yeast identification: evaluation of their specificity and performance

Detalhes bibliográficos
Autor(a) principal: Branco, Patricia
Data de Publicação: 2017
Outros Autores: Candeias, António, Caldeira, Ana Teresa , González-Pérez, Marina
Tipo de documento: Artigo de conferência
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10174/21746
Resumo: The aim of this work was to investigate the possibility of applying the RNA-FISH protocol previously developed by our group for simultaneous analysis of fungi and bacteria using kingdom-specific probes for specific detection of various yeast species using previously published species-specific probes. Their specificity and performance were analysed both in silico and experimentally.
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spelling Species-specific RNA-FISH probes for yeast identification: evaluation of their specificity and performanceRNA-FISHprobes specificityyeastprobes performanceThe aim of this work was to investigate the possibility of applying the RNA-FISH protocol previously developed by our group for simultaneous analysis of fungi and bacteria using kingdom-specific probes for specific detection of various yeast species using previously published species-specific probes. Their specificity and performance were analysed both in silico and experimentally.FCT through PTDC/BBB-IMG/0046/2014 project and SFRH/BPD/100754/2014 grant and ALT20-03-0246-FEDER-000004-ALENTEJO 2020 projectSociedade Portuguesa de Microbiologia; Sociedade Portuguesa de Biotecnología; Católica Escola Superior de Biotecnologia, Porto;,2018-01-08T12:34:38Z2018-01-082017-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjecthttp://hdl.handle.net/10174/21746http://hdl.handle.net/10174/21746enghttp://www.porto.ucp.pt/sites/default/files/files/Biotecnologia/Microbiotec17/BookofAbstracts_15_12_2017.pdfnaonaosimpbranco@uevora.ptcandeias@uevora.ptatc@uevora.ptmarinagp@uevora.pt371Branco, PatriciaCandeias, AntónioCaldeira, Ana Teresa González-Pérez, Marinainfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-01-03T19:12:40Zoai:dspace.uevora.pt:10174/21746Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T01:12:59.093329Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Species-specific RNA-FISH probes for yeast identification: evaluation of their specificity and performance
title Species-specific RNA-FISH probes for yeast identification: evaluation of their specificity and performance
spellingShingle Species-specific RNA-FISH probes for yeast identification: evaluation of their specificity and performance
Branco, Patricia
RNA-FISH
probes specificity
yeast
probes performance
title_short Species-specific RNA-FISH probes for yeast identification: evaluation of their specificity and performance
title_full Species-specific RNA-FISH probes for yeast identification: evaluation of their specificity and performance
title_fullStr Species-specific RNA-FISH probes for yeast identification: evaluation of their specificity and performance
title_full_unstemmed Species-specific RNA-FISH probes for yeast identification: evaluation of their specificity and performance
title_sort Species-specific RNA-FISH probes for yeast identification: evaluation of their specificity and performance
author Branco, Patricia
author_facet Branco, Patricia
Candeias, António
Caldeira, Ana Teresa 
González-Pérez, Marina
author_role author
author2 Candeias, António
Caldeira, Ana Teresa 
González-Pérez, Marina
author2_role author
author
author
dc.contributor.author.fl_str_mv Branco, Patricia
Candeias, António
Caldeira, Ana Teresa 
González-Pérez, Marina
dc.subject.por.fl_str_mv RNA-FISH
probes specificity
yeast
probes performance
topic RNA-FISH
probes specificity
yeast
probes performance
description The aim of this work was to investigate the possibility of applying the RNA-FISH protocol previously developed by our group for simultaneous analysis of fungi and bacteria using kingdom-specific probes for specific detection of various yeast species using previously published species-specific probes. Their specificity and performance were analysed both in silico and experimentally.
publishDate 2017
dc.date.none.fl_str_mv 2017-01-01T00:00:00Z
2018-01-08T12:34:38Z
2018-01-08
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/conferenceObject
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status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10174/21746
http://hdl.handle.net/10174/21746
url http://hdl.handle.net/10174/21746
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv http://www.porto.ucp.pt/sites/default/files/files/Biotecnologia/Microbiotec17/BookofAbstracts_15_12_2017.pdf
nao
nao
sim
pbranco@uevora.pt
candeias@uevora.pt
atc@uevora.pt
marinagp@uevora.pt
371
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Sociedade Portuguesa de Microbiologia; Sociedade Portuguesa de Biotecnología; Católica Escola Superior de Biotecnologia, Porto;,
publisher.none.fl_str_mv Sociedade Portuguesa de Microbiologia; Sociedade Portuguesa de Biotecnología; Católica Escola Superior de Biotecnologia, Porto;,
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
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instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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