Structure of Tetrahymena CCTθ gene and its expression under colchicine treatment
Autor(a) principal: | |
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Data de Publicação: | 1999 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.21/8877 |
Resumo: | We report here the cloning and the characterization of the Tetrahymena pyriformis chaperonin-containing-TCP1 theta gene (TpCCTθ), an orthologue of the mouse chaperonin gene CCTθ. TpCCTθ gene is interrupted by eight introns, ranging in size between 91 and 419 nucleotides, and encodes a protein consisting of 540 amino acid residues (59.1 kDa), with a putative pI of 5.73. The amino acid sequence of TpCCTθ reveals 39.4–46.0% identity with the sequences of Candida albicans and mouse CCTθ subunits and 28.0–32.6% identity with the other TpCCT subunits known so far. We have studied the expression of this gene in exponentially growing Tetrahymena cells and in cells treated with colchicine for different times. The steady-state levels of CCTθ mRNA rapidly decrease in the first 30 min of colchicine treatment. Interestingly, treatment for subsequent 60 min gives expression levels higher than those found in exponentially growing cells. |
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Structure of Tetrahymena CCTθ gene and its expression under colchicine treatmentCCTθ-chaperonin geneCiliated protozoanGene expressionColchicineAmino acid sequenceGene expression regulationMolecular sequence dataSequence alignmentWe report here the cloning and the characterization of the Tetrahymena pyriformis chaperonin-containing-TCP1 theta gene (TpCCTθ), an orthologue of the mouse chaperonin gene CCTθ. TpCCTθ gene is interrupted by eight introns, ranging in size between 91 and 419 nucleotides, and encodes a protein consisting of 540 amino acid residues (59.1 kDa), with a putative pI of 5.73. The amino acid sequence of TpCCTθ reveals 39.4–46.0% identity with the sequences of Candida albicans and mouse CCTθ subunits and 28.0–32.6% identity with the other TpCCT subunits known so far. We have studied the expression of this gene in exponentially growing Tetrahymena cells and in cells treated with colchicine for different times. The steady-state levels of CCTθ mRNA rapidly decrease in the first 30 min of colchicine treatment. Interestingly, treatment for subsequent 60 min gives expression levels higher than those found in exponentially growing cells.ElsevierRCIPLDomingues, CéliaSoares, HelenaRodrigues-Pousada, ClaudinaCyrne, Luisa2018-09-21T12:47:46Z1999-091999-09-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.21/8877engDomingues C, Soares H, Rodrigues-Pousada C, Cyrne L. Structure of Tetrahymena CCTθ gene and its expression under colchicine treatment. Biochim Biophys Acta. 1999;1446(3):443-9.10.1016/S0167-4781(99)00111-6info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-08-03T09:56:53Zoai:repositorio.ipl.pt:10400.21/8877Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T20:17:34.554159Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Structure of Tetrahymena CCTθ gene and its expression under colchicine treatment |
title |
Structure of Tetrahymena CCTθ gene and its expression under colchicine treatment |
spellingShingle |
Structure of Tetrahymena CCTθ gene and its expression under colchicine treatment Domingues, Célia CCTθ-chaperonin gene Ciliated protozoan Gene expression Colchicine Amino acid sequence Gene expression regulation Molecular sequence data Sequence alignment |
title_short |
Structure of Tetrahymena CCTθ gene and its expression under colchicine treatment |
title_full |
Structure of Tetrahymena CCTθ gene and its expression under colchicine treatment |
title_fullStr |
Structure of Tetrahymena CCTθ gene and its expression under colchicine treatment |
title_full_unstemmed |
Structure of Tetrahymena CCTθ gene and its expression under colchicine treatment |
title_sort |
Structure of Tetrahymena CCTθ gene and its expression under colchicine treatment |
author |
Domingues, Célia |
author_facet |
Domingues, Célia Soares, Helena Rodrigues-Pousada, Claudina Cyrne, Luisa |
author_role |
author |
author2 |
Soares, Helena Rodrigues-Pousada, Claudina Cyrne, Luisa |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
RCIPL |
dc.contributor.author.fl_str_mv |
Domingues, Célia Soares, Helena Rodrigues-Pousada, Claudina Cyrne, Luisa |
dc.subject.por.fl_str_mv |
CCTθ-chaperonin gene Ciliated protozoan Gene expression Colchicine Amino acid sequence Gene expression regulation Molecular sequence data Sequence alignment |
topic |
CCTθ-chaperonin gene Ciliated protozoan Gene expression Colchicine Amino acid sequence Gene expression regulation Molecular sequence data Sequence alignment |
description |
We report here the cloning and the characterization of the Tetrahymena pyriformis chaperonin-containing-TCP1 theta gene (TpCCTθ), an orthologue of the mouse chaperonin gene CCTθ. TpCCTθ gene is interrupted by eight introns, ranging in size between 91 and 419 nucleotides, and encodes a protein consisting of 540 amino acid residues (59.1 kDa), with a putative pI of 5.73. The amino acid sequence of TpCCTθ reveals 39.4–46.0% identity with the sequences of Candida albicans and mouse CCTθ subunits and 28.0–32.6% identity with the other TpCCT subunits known so far. We have studied the expression of this gene in exponentially growing Tetrahymena cells and in cells treated with colchicine for different times. The steady-state levels of CCTθ mRNA rapidly decrease in the first 30 min of colchicine treatment. Interestingly, treatment for subsequent 60 min gives expression levels higher than those found in exponentially growing cells. |
publishDate |
1999 |
dc.date.none.fl_str_mv |
1999-09 1999-09-01T00:00:00Z 2018-09-21T12:47:46Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.21/8877 |
url |
http://hdl.handle.net/10400.21/8877 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Domingues C, Soares H, Rodrigues-Pousada C, Cyrne L. Structure of Tetrahymena CCTθ gene and its expression under colchicine treatment. Biochim Biophys Acta. 1999;1446(3):443-9. 10.1016/S0167-4781(99)00111-6 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799133438628855808 |