Candida albicans antimicrobial and antibiofilm activity of novel endodontic solvents
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/73906 |
Resumo: | Background: Candida albicans is the most prevalent fungi isolated in endodontic infections. In this study, the ability of C. albicans biofilm to tolerate the novel solvent mixtures methyl ethyl ketone (MEK)/tetrachloroethylene (TCE) and MEK/orange oil (OOil) sequentially to the standard irrigation of sodium hypochlorite (NaOCl) and ethylenediaminetetraacetic (EDTA) was evaluated. Methods: Biofilm cell cultures of C. albicans SC 5314 were treated sequentially with NaOCl and EDTA and exposed to MEK/TCE or MEK/OOil. The effect of the antimicrobial treatment was evaluated using the disk diffusion method for planktonic culture, and the enumeration of colony-forming units (CFUs) and scanning electron microscope (SEM) for biofilm cell culture. Results: C. albicans mature biofilm (24 h) was significantly reduced in biomass and cell viability after solvent mixtures exposure, compared with the previous NaOCl and EDTA treatments. MEK/OOil combination caused a total reduction of biofilm, while with MEK/TCE, there was a 3-log (CFU/cm2) reduction compared with the sequence NaOCl and EDTA, and a 4-log (CFU/cm2) reduction compared with the control. Conclusions: The additional exposure of a preformed 24 h C. albicans biofilm to novel solvent mixtures MEK/TCE and MEK/OOil caused a positive antibiofilm impact, overcoming the performance of the conventional endodontic irrigating protocol. |
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Candida albicans antimicrobial and antibiofilm activity of novel endodontic solventsAdjunctive approachesAntibiofilmAntimicrobialCandida albicansEndodontic infectionsEndodontic solventsEndodontic retreatmentIrrigating solutionsRoot canal infectionSolvent mixturesCiências Naturais::Ciências BiológicasScience & TechnologyBackground: Candida albicans is the most prevalent fungi isolated in endodontic infections. In this study, the ability of C. albicans biofilm to tolerate the novel solvent mixtures methyl ethyl ketone (MEK)/tetrachloroethylene (TCE) and MEK/orange oil (OOil) sequentially to the standard irrigation of sodium hypochlorite (NaOCl) and ethylenediaminetetraacetic (EDTA) was evaluated. Methods: Biofilm cell cultures of C. albicans SC 5314 were treated sequentially with NaOCl and EDTA and exposed to MEK/TCE or MEK/OOil. The effect of the antimicrobial treatment was evaluated using the disk diffusion method for planktonic culture, and the enumeration of colony-forming units (CFUs) and scanning electron microscope (SEM) for biofilm cell culture. Results: C. albicans mature biofilm (24 h) was significantly reduced in biomass and cell viability after solvent mixtures exposure, compared with the previous NaOCl and EDTA treatments. MEK/OOil combination caused a total reduction of biofilm, while with MEK/TCE, there was a 3-log (CFU/cm2) reduction compared with the sequence NaOCl and EDTA, and a 4-log (CFU/cm2) reduction compared with the control. Conclusions: The additional exposure of a preformed 24 h C. albicans biofilm to novel solvent mixtures MEK/TCE and MEK/OOil caused a positive antibiofilm impact, overcoming the performance of the conventional endodontic irrigating protocol.This article was supported by National Funds through FCT-Fundação para a Ciência e a Tecnologia, I.P., within CINTESIS, R&D Unit (reference UIDB/4255/2020). This work was supported by the Portuguese Foundation for Science and Technology (FCT) under the scope of the strategic funding of UIDB/04469/2020 unit and grant ref 2020.05720.BD for Liliana Fernandes; BioTecNorte operation (NORTE-01-0145-FEDER-000004) funded by the European Regional Development Fund under the scope of Norte2020-Programa Operacional Regional do Norte.info:eu-repo/semantics/publishedVersionMDPIUniversidade do MinhoFerreira, InêsRodrigues, M. ElisaFernandes, Liliana SousaHenriques, MarianaPina-Vaz, Irene2021-08-232021-08-23T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/73906engFerreira, Inês; Rodrigues, M. Elisa; Fernandes, L.; Henriques, Mariana; Pina-Vaz, Irene, Candida albicans antimicrobial and antibiofilm activity of novel endodontic solvents. Applied Sciences, 11(16), 7748, 20212076-341710.3390/app11167748https://www.mdpi.com/2076-3417/11/16/7748info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:12:45Zoai:repositorium.sdum.uminho.pt:1822/73906Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:04:42.300624Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Candida albicans antimicrobial and antibiofilm activity of novel endodontic solvents |
title |
Candida albicans antimicrobial and antibiofilm activity of novel endodontic solvents |
spellingShingle |
Candida albicans antimicrobial and antibiofilm activity of novel endodontic solvents Ferreira, Inês Adjunctive approaches Antibiofilm Antimicrobial Candida albicans Endodontic infections Endodontic solvents Endodontic retreatment Irrigating solutions Root canal infection Solvent mixtures Ciências Naturais::Ciências Biológicas Science & Technology |
title_short |
Candida albicans antimicrobial and antibiofilm activity of novel endodontic solvents |
title_full |
Candida albicans antimicrobial and antibiofilm activity of novel endodontic solvents |
title_fullStr |
Candida albicans antimicrobial and antibiofilm activity of novel endodontic solvents |
title_full_unstemmed |
Candida albicans antimicrobial and antibiofilm activity of novel endodontic solvents |
title_sort |
Candida albicans antimicrobial and antibiofilm activity of novel endodontic solvents |
author |
Ferreira, Inês |
author_facet |
Ferreira, Inês Rodrigues, M. Elisa Fernandes, Liliana Sousa Henriques, Mariana Pina-Vaz, Irene |
author_role |
author |
author2 |
Rodrigues, M. Elisa Fernandes, Liliana Sousa Henriques, Mariana Pina-Vaz, Irene |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Ferreira, Inês Rodrigues, M. Elisa Fernandes, Liliana Sousa Henriques, Mariana Pina-Vaz, Irene |
dc.subject.por.fl_str_mv |
Adjunctive approaches Antibiofilm Antimicrobial Candida albicans Endodontic infections Endodontic solvents Endodontic retreatment Irrigating solutions Root canal infection Solvent mixtures Ciências Naturais::Ciências Biológicas Science & Technology |
topic |
Adjunctive approaches Antibiofilm Antimicrobial Candida albicans Endodontic infections Endodontic solvents Endodontic retreatment Irrigating solutions Root canal infection Solvent mixtures Ciências Naturais::Ciências Biológicas Science & Technology |
description |
Background: Candida albicans is the most prevalent fungi isolated in endodontic infections. In this study, the ability of C. albicans biofilm to tolerate the novel solvent mixtures methyl ethyl ketone (MEK)/tetrachloroethylene (TCE) and MEK/orange oil (OOil) sequentially to the standard irrigation of sodium hypochlorite (NaOCl) and ethylenediaminetetraacetic (EDTA) was evaluated. Methods: Biofilm cell cultures of C. albicans SC 5314 were treated sequentially with NaOCl and EDTA and exposed to MEK/TCE or MEK/OOil. The effect of the antimicrobial treatment was evaluated using the disk diffusion method for planktonic culture, and the enumeration of colony-forming units (CFUs) and scanning electron microscope (SEM) for biofilm cell culture. Results: C. albicans mature biofilm (24 h) was significantly reduced in biomass and cell viability after solvent mixtures exposure, compared with the previous NaOCl and EDTA treatments. MEK/OOil combination caused a total reduction of biofilm, while with MEK/TCE, there was a 3-log (CFU/cm2) reduction compared with the sequence NaOCl and EDTA, and a 4-log (CFU/cm2) reduction compared with the control. Conclusions: The additional exposure of a preformed 24 h C. albicans biofilm to novel solvent mixtures MEK/TCE and MEK/OOil caused a positive antibiofilm impact, overcoming the performance of the conventional endodontic irrigating protocol. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-08-23 2021-08-23T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/73906 |
url |
http://hdl.handle.net/1822/73906 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Ferreira, Inês; Rodrigues, M. Elisa; Fernandes, L.; Henriques, Mariana; Pina-Vaz, Irene, Candida albicans antimicrobial and antibiofilm activity of novel endodontic solvents. Applied Sciences, 11(16), 7748, 2021 2076-3417 10.3390/app11167748 https://www.mdpi.com/2076-3417/11/16/7748 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
MDPI |
publisher.none.fl_str_mv |
MDPI |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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