Olive pomace phenolic compounds stability and safety evaluation: From raw material to future ophthalmic applications

Detalhes bibliográficos
Autor(a) principal: Katsinas, Nikolaos
Data de Publicação: 2021
Outros Autores: Enríquez-De-salamanca, Amalia, Silva, Andreia Bento da, Bronze, Maria Rosário, Rodríguez-Rojo, Soraya
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/131330
Resumo: H2020-MSCA-ITN-2017 01 0145 FEDER 402 022125
id RCAP_242af6716f7f72a25f1959646b9a4d9b
oai_identifier_str oai:run.unl.pt:10362/131330
network_acronym_str RCAP
network_name_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository_id_str 7160
spelling Olive pomace phenolic compounds stability and safety evaluation: From raw material to future ophthalmic applicationsGenotoxicity assayHydroxytyrosolOleuropeinOlive pomacePhenolic extractsStorage stabilityAnalytical ChemistryChemistry (miscellaneous)Molecular MedicinePharmaceutical ScienceDrug DiscoveryPhysical and Theoretical ChemistryOrganic ChemistryH2020-MSCA-ITN-2017 01 0145 FEDER 402 022125Nowadays, increasing interest in olive pomace (OP) valorization aims to improve olive’s industry sustainability. Interestingly, several studies propose a high-value application for OP extracts containing its main phenolic compounds, hydroxytyrosol and oleuropein, as therapy for ocular surface diseases. In this work, the stability and accessibility of OP total phenolic and flavonoid content, main representative compounds, and antioxidant activity were assessed under different pretreatment conditions. Among them, lyophilization and supercritical CO2 extraction were found to increase significantly most responses measured in the produced extracts. Two selected extracts (CONV and OPT3) were obtained by different techniques (conventional and pressurized liquid ex-traction); Their aqueous solutions were characterized by HPLC-DAD-MS/MS. Additionally, their safety and stability were evaluated according to EMA requirements towards their approval as ophthalmic products: their genotoxic effect on ocular surface cells and their 6-months storage stability at 4 different temperature/moisture conditions (CPMP/ICH/2736/99), together with pure hydroxy-tyrosol and oleuropein solutions. The concentration of hydroxytyrosol and oleuropein in pure or extract solutions was tracked, and possible degradation products were putatively identified by HPLC-DAD-MS/MS. Hydroxytyrosol and oleuropein had different stability as standard or extract solutions, with oleuropein also showing different degradation profile. All compounds/extracts were safe for ophthalmic use at the concentrations tested.DCT - Departamento de Ciências da TerraInstituto de Tecnologia Química e Biológica António Xavier (ITQB)RUNKatsinas, NikolaosEnríquez-De-salamanca, AmaliaSilva, Andreia Bento daBronze, Maria RosárioRodríguez-Rojo, Soraya2022-01-22T03:41:33Z2021-10-022021-10-02T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10362/131330eng1420-3049PURE: 35917588https://doi.org/10.3390/molecules26196002info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T05:09:58Zoai:run.unl.pt:10362/131330Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:47:03.115778Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Olive pomace phenolic compounds stability and safety evaluation: From raw material to future ophthalmic applications
title Olive pomace phenolic compounds stability and safety evaluation: From raw material to future ophthalmic applications
spellingShingle Olive pomace phenolic compounds stability and safety evaluation: From raw material to future ophthalmic applications
Katsinas, Nikolaos
Genotoxicity assay
Hydroxytyrosol
Oleuropein
Olive pomace
Phenolic extracts
Storage stability
Analytical Chemistry
Chemistry (miscellaneous)
Molecular Medicine
Pharmaceutical Science
Drug Discovery
Physical and Theoretical Chemistry
Organic Chemistry
title_short Olive pomace phenolic compounds stability and safety evaluation: From raw material to future ophthalmic applications
title_full Olive pomace phenolic compounds stability and safety evaluation: From raw material to future ophthalmic applications
title_fullStr Olive pomace phenolic compounds stability and safety evaluation: From raw material to future ophthalmic applications
title_full_unstemmed Olive pomace phenolic compounds stability and safety evaluation: From raw material to future ophthalmic applications
title_sort Olive pomace phenolic compounds stability and safety evaluation: From raw material to future ophthalmic applications
author Katsinas, Nikolaos
author_facet Katsinas, Nikolaos
Enríquez-De-salamanca, Amalia
Silva, Andreia Bento da
Bronze, Maria Rosário
Rodríguez-Rojo, Soraya
author_role author
author2 Enríquez-De-salamanca, Amalia
Silva, Andreia Bento da
Bronze, Maria Rosário
Rodríguez-Rojo, Soraya
author2_role author
author
author
author
dc.contributor.none.fl_str_mv DCT - Departamento de Ciências da Terra
Instituto de Tecnologia Química e Biológica António Xavier (ITQB)
RUN
dc.contributor.author.fl_str_mv Katsinas, Nikolaos
Enríquez-De-salamanca, Amalia
Silva, Andreia Bento da
Bronze, Maria Rosário
Rodríguez-Rojo, Soraya
dc.subject.por.fl_str_mv Genotoxicity assay
Hydroxytyrosol
Oleuropein
Olive pomace
Phenolic extracts
Storage stability
Analytical Chemistry
Chemistry (miscellaneous)
Molecular Medicine
Pharmaceutical Science
Drug Discovery
Physical and Theoretical Chemistry
Organic Chemistry
topic Genotoxicity assay
Hydroxytyrosol
Oleuropein
Olive pomace
Phenolic extracts
Storage stability
Analytical Chemistry
Chemistry (miscellaneous)
Molecular Medicine
Pharmaceutical Science
Drug Discovery
Physical and Theoretical Chemistry
Organic Chemistry
description H2020-MSCA-ITN-2017 01 0145 FEDER 402 022125
publishDate 2021
dc.date.none.fl_str_mv 2021-10-02
2021-10-02T00:00:00Z
2022-01-22T03:41:33Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/131330
url http://hdl.handle.net/10362/131330
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 1420-3049
PURE: 35917588
https://doi.org/10.3390/molecules26196002
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
_version_ 1799138073915686912

Registros relacionados