Filago pyramidata tolerant to ALS-Inhibiting herbicides: A new invasive weed in olive groves of Southern Spain
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | https://hdl.handle.net/20.500.12207/6063 |
Resumo: | Weeds that usually grow in non-agricultural areas have become increasingly common invading perennial crops. Species of the genus Filago, in addition to invading Spanish olive groves, have developed certain levels of natural tolerance to the acetolactate synthase (ALS) inhibiting herbicide flazasulfuron. The objective of this study was to determine the level and the mechanism involved in the tolerance to flazasulfuron in Filago pyramidata L., which occurs in olive groves of southern Spain, as well as to identify possible cross- or multiple-tolerances by evaluating alternative herbicides for its control. A population resistant (R) to flazasulfuron and a susceptible (S) one of Conyza canadensis were used as references. The accessions of F. pyramidata presented LD50 values (from 72 to 81 g active ingredient (ai) ha−1) higher than the field dose of flazasulfuron (50 g ai ha−1), being 11–12.5 times more tolerant than the S population of C. canadensis, but less than half the R population (170 g ai ha−1). Enzymatically, F. pyramidata was as sensitive to flazasulfuron (I50 = 17.3 μM) as the S population of C. canadensis. Filago pyramidata plants treated with flazasulfuron, combined with 4-chloro-7-nitro-2,1,3-benzoxadiazole, had a growth reduction of up to 85%, revealing the participation of glutathione-S-transferases in herbicide metabolism. Filago pyramidata presented cross-tolerance to the different chemical groups of ALS inhibitors, except triazolinones (florasulam). Synthetic auxins (2,4-D and fluroxypyr) presented good control, but some individuals survived (low multiple resistance). Cellulose synthesis, 5-enolpyruvylshikimate-3-phosphate, 4-hydroxyphenylpyruvate dioxygenase, protoporphyrinogen oxidase, photosystem I, and photosystem II inhibitor herbicides, applied in PRE or POST-emergence, presented excellent levels of control of F. pyramidata. These results confirmed the natural tolerance of F. pyramidata to flazasulfuron and cross-tolerance to most ALS-inhibiting herbicides. The mechanism involved was enhanced metabolism mediated by glutathione-S-transferases, which also conferred low multiple tolerance to synthetic auxins. Even so, herbicides with other mechanisms of action still offer excellent levels of control of F. pyramidata. |
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Filago pyramidata tolerant to ALS-Inhibiting herbicides: A new invasive weed in olive groves of Southern Spain4-chloro-7-nitro-2,1,3-benzoxadiazoleCottonroseGlutathione-S-transferasesHerbicide metabolismHerbicide toleranceTerafitWeeds that usually grow in non-agricultural areas have become increasingly common invading perennial crops. Species of the genus Filago, in addition to invading Spanish olive groves, have developed certain levels of natural tolerance to the acetolactate synthase (ALS) inhibiting herbicide flazasulfuron. The objective of this study was to determine the level and the mechanism involved in the tolerance to flazasulfuron in Filago pyramidata L., which occurs in olive groves of southern Spain, as well as to identify possible cross- or multiple-tolerances by evaluating alternative herbicides for its control. A population resistant (R) to flazasulfuron and a susceptible (S) one of Conyza canadensis were used as references. The accessions of F. pyramidata presented LD50 values (from 72 to 81 g active ingredient (ai) ha−1) higher than the field dose of flazasulfuron (50 g ai ha−1), being 11–12.5 times more tolerant than the S population of C. canadensis, but less than half the R population (170 g ai ha−1). Enzymatically, F. pyramidata was as sensitive to flazasulfuron (I50 = 17.3 μM) as the S population of C. canadensis. Filago pyramidata plants treated with flazasulfuron, combined with 4-chloro-7-nitro-2,1,3-benzoxadiazole, had a growth reduction of up to 85%, revealing the participation of glutathione-S-transferases in herbicide metabolism. Filago pyramidata presented cross-tolerance to the different chemical groups of ALS inhibitors, except triazolinones (florasulam). Synthetic auxins (2,4-D and fluroxypyr) presented good control, but some individuals survived (low multiple resistance). Cellulose synthesis, 5-enolpyruvylshikimate-3-phosphate, 4-hydroxyphenylpyruvate dioxygenase, protoporphyrinogen oxidase, photosystem I, and photosystem II inhibitor herbicides, applied in PRE or POST-emergence, presented excellent levels of control of F. pyramidata. These results confirmed the natural tolerance of F. pyramidata to flazasulfuron and cross-tolerance to most ALS-inhibiting herbicides. The mechanism involved was enhanced metabolism mediated by glutathione-S-transferases, which also conferred low multiple tolerance to synthetic auxins. Even so, herbicides with other mechanisms of action still offer excellent levels of control of F. pyramidata.MDPI2023-11-30T11:16:47Z2023-04-28T00:00:00Z2023-04-28info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/20.500.12207/6063eng2073-4395https://doi.org/10.3390/agronomy13051273Palma-Bautista, CandelarioVásquez-García, JoséDomínguez-Valenzuela, JoséAlcántara-de la Cruz, RicardoDe Prado, RafaelPortugal, Joãoinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-07T09:10:10Zoai:repositorio.ipbeja.pt:20.500.12207/6063Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T00:41:28.665042Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Filago pyramidata tolerant to ALS-Inhibiting herbicides: A new invasive weed in olive groves of Southern Spain |
title |
Filago pyramidata tolerant to ALS-Inhibiting herbicides: A new invasive weed in olive groves of Southern Spain |
spellingShingle |
Filago pyramidata tolerant to ALS-Inhibiting herbicides: A new invasive weed in olive groves of Southern Spain Palma-Bautista, Candelario 4-chloro-7-nitro-2,1,3-benzoxadiazole Cottonrose Glutathione-S-transferases Herbicide metabolism Herbicide tolerance Terafit |
title_short |
Filago pyramidata tolerant to ALS-Inhibiting herbicides: A new invasive weed in olive groves of Southern Spain |
title_full |
Filago pyramidata tolerant to ALS-Inhibiting herbicides: A new invasive weed in olive groves of Southern Spain |
title_fullStr |
Filago pyramidata tolerant to ALS-Inhibiting herbicides: A new invasive weed in olive groves of Southern Spain |
title_full_unstemmed |
Filago pyramidata tolerant to ALS-Inhibiting herbicides: A new invasive weed in olive groves of Southern Spain |
title_sort |
Filago pyramidata tolerant to ALS-Inhibiting herbicides: A new invasive weed in olive groves of Southern Spain |
author |
Palma-Bautista, Candelario |
author_facet |
Palma-Bautista, Candelario Vásquez-García, José Domínguez-Valenzuela, José Alcántara-de la Cruz, Ricardo De Prado, Rafael Portugal, João |
author_role |
author |
author2 |
Vásquez-García, José Domínguez-Valenzuela, José Alcántara-de la Cruz, Ricardo De Prado, Rafael Portugal, João |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Palma-Bautista, Candelario Vásquez-García, José Domínguez-Valenzuela, José Alcántara-de la Cruz, Ricardo De Prado, Rafael Portugal, João |
dc.subject.por.fl_str_mv |
4-chloro-7-nitro-2,1,3-benzoxadiazole Cottonrose Glutathione-S-transferases Herbicide metabolism Herbicide tolerance Terafit |
topic |
4-chloro-7-nitro-2,1,3-benzoxadiazole Cottonrose Glutathione-S-transferases Herbicide metabolism Herbicide tolerance Terafit |
description |
Weeds that usually grow in non-agricultural areas have become increasingly common invading perennial crops. Species of the genus Filago, in addition to invading Spanish olive groves, have developed certain levels of natural tolerance to the acetolactate synthase (ALS) inhibiting herbicide flazasulfuron. The objective of this study was to determine the level and the mechanism involved in the tolerance to flazasulfuron in Filago pyramidata L., which occurs in olive groves of southern Spain, as well as to identify possible cross- or multiple-tolerances by evaluating alternative herbicides for its control. A population resistant (R) to flazasulfuron and a susceptible (S) one of Conyza canadensis were used as references. The accessions of F. pyramidata presented LD50 values (from 72 to 81 g active ingredient (ai) ha−1) higher than the field dose of flazasulfuron (50 g ai ha−1), being 11–12.5 times more tolerant than the S population of C. canadensis, but less than half the R population (170 g ai ha−1). Enzymatically, F. pyramidata was as sensitive to flazasulfuron (I50 = 17.3 μM) as the S population of C. canadensis. Filago pyramidata plants treated with flazasulfuron, combined with 4-chloro-7-nitro-2,1,3-benzoxadiazole, had a growth reduction of up to 85%, revealing the participation of glutathione-S-transferases in herbicide metabolism. Filago pyramidata presented cross-tolerance to the different chemical groups of ALS inhibitors, except triazolinones (florasulam). Synthetic auxins (2,4-D and fluroxypyr) presented good control, but some individuals survived (low multiple resistance). Cellulose synthesis, 5-enolpyruvylshikimate-3-phosphate, 4-hydroxyphenylpyruvate dioxygenase, protoporphyrinogen oxidase, photosystem I, and photosystem II inhibitor herbicides, applied in PRE or POST-emergence, presented excellent levels of control of F. pyramidata. These results confirmed the natural tolerance of F. pyramidata to flazasulfuron and cross-tolerance to most ALS-inhibiting herbicides. The mechanism involved was enhanced metabolism mediated by glutathione-S-transferases, which also conferred low multiple tolerance to synthetic auxins. Even so, herbicides with other mechanisms of action still offer excellent levels of control of F. pyramidata. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-11-30T11:16:47Z 2023-04-28T00:00:00Z 2023-04-28 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://hdl.handle.net/20.500.12207/6063 |
url |
https://hdl.handle.net/20.500.12207/6063 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
2073-4395 https://doi.org/10.3390/agronomy13051273 |
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info:eu-repo/semantics/openAccess |
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openAccess |
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application/pdf |
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MDPI |
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MDPI |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799136317866508288 |