Oxidative stress adaptation in aggressive prostate cancer may be counteracted by the reduction of glutathione reductase

Detalhes bibliográficos
Autor(a) principal: Freitas, Mariana
Data de Publicação: 2012
Outros Autores: Baldeiras, Inês, Proença, Teresa, Alves, Vera, Mota-Pinto, Anabela, Sarmento-Ribeiro, Ana Bela
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10316/20296
https://doi.org/10.1016/j.fob.2012.05.001
Resumo: Oxidative stress has been associated with prostate cancer development and progression due to an increase of reactive oxygen species (ROS). However, the mechanisms whereby ROS and the antioxidant system participate in cancer progression remain unclear. In order to clarify the influence of oxidative stress in prostate cancer progression, we performed this study in two human prostate cancer cell lines, PC3 and HPV10 (from metastasis and from localized cancer, respectively) and RWPE1 cells derived from normal prostate epithelium. Cells were treated with hydrogen peroxide (H2O2) and PC3 cells were also treated with diethyl maleate (DEM). The effect on cell growth, viability, mitochondria membrane potential and oxidative stress was analysed. Oxidative stress was evaluated based on ROS production, oxidative lesion of lipids (MDA) and on determination of antioxidants, including enzyme activity of glutathione peroxidase (Gl-Px), glutathione reductase (Gl-Red) and on the quantification of glutathione (GSH), glutathione-s-transferase (GST) and total antioxidant status (TAS). PC3 shows higher ROS production but also the highest GSH levels and Gl-Red activity, possibly contributing to oxidative stress resistance. This is also associated with higher mitochondrial membrane potential, TAS and lower lipid peroxidation. On the other hand, we identified Gl-Red activity reduction as a new strategy in overcoming oxidative stress resistance, by inducing H2O2 cytotoxicity. Therefore these results suggest Gl-Red activity reduction as a new potential therapeutic approach, in prostate cancer.
id RCAP_247dfa09b09cdb17233aa23186c0cb25
oai_identifier_str oai:estudogeral.uc.pt:10316/20296
network_acronym_str RCAP
network_name_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository_id_str 7160
spelling Oxidative stress adaptation in aggressive prostate cancer may be counteracted by the reduction of glutathione reductaseStresse OxidativoCancro da próstataOxidative stress has been associated with prostate cancer development and progression due to an increase of reactive oxygen species (ROS). However, the mechanisms whereby ROS and the antioxidant system participate in cancer progression remain unclear. In order to clarify the influence of oxidative stress in prostate cancer progression, we performed this study in two human prostate cancer cell lines, PC3 and HPV10 (from metastasis and from localized cancer, respectively) and RWPE1 cells derived from normal prostate epithelium. Cells were treated with hydrogen peroxide (H2O2) and PC3 cells were also treated with diethyl maleate (DEM). The effect on cell growth, viability, mitochondria membrane potential and oxidative stress was analysed. Oxidative stress was evaluated based on ROS production, oxidative lesion of lipids (MDA) and on determination of antioxidants, including enzyme activity of glutathione peroxidase (Gl-Px), glutathione reductase (Gl-Red) and on the quantification of glutathione (GSH), glutathione-s-transferase (GST) and total antioxidant status (TAS). PC3 shows higher ROS production but also the highest GSH levels and Gl-Red activity, possibly contributing to oxidative stress resistance. This is also associated with higher mitochondrial membrane potential, TAS and lower lipid peroxidation. On the other hand, we identified Gl-Red activity reduction as a new strategy in overcoming oxidative stress resistance, by inducing H2O2 cytotoxicity. Therefore these results suggest Gl-Red activity reduction as a new potential therapeutic approach, in prostate cancer.2012info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://hdl.handle.net/10316/20296http://hdl.handle.net/10316/20296https://doi.org/10.1016/j.fob.2012.05.001enghttp://www.sciencedirect.com/science/article/pii/S2211546312000186Freitas, MarianaBaldeiras, InêsProença, TeresaAlves, VeraMota-Pinto, AnabelaSarmento-Ribeiro, Ana Belainfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2022-05-25T05:12:21Zoai:estudogeral.uc.pt:10316/20296Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T20:43:39.147500Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Oxidative stress adaptation in aggressive prostate cancer may be counteracted by the reduction of glutathione reductase
title Oxidative stress adaptation in aggressive prostate cancer may be counteracted by the reduction of glutathione reductase
spellingShingle Oxidative stress adaptation in aggressive prostate cancer may be counteracted by the reduction of glutathione reductase
Freitas, Mariana
Stresse Oxidativo
Cancro da próstata
title_short Oxidative stress adaptation in aggressive prostate cancer may be counteracted by the reduction of glutathione reductase
title_full Oxidative stress adaptation in aggressive prostate cancer may be counteracted by the reduction of glutathione reductase
title_fullStr Oxidative stress adaptation in aggressive prostate cancer may be counteracted by the reduction of glutathione reductase
title_full_unstemmed Oxidative stress adaptation in aggressive prostate cancer may be counteracted by the reduction of glutathione reductase
title_sort Oxidative stress adaptation in aggressive prostate cancer may be counteracted by the reduction of glutathione reductase
author Freitas, Mariana
author_facet Freitas, Mariana
Baldeiras, Inês
Proença, Teresa
Alves, Vera
Mota-Pinto, Anabela
Sarmento-Ribeiro, Ana Bela
author_role author
author2 Baldeiras, Inês
Proença, Teresa
Alves, Vera
Mota-Pinto, Anabela
Sarmento-Ribeiro, Ana Bela
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Freitas, Mariana
Baldeiras, Inês
Proença, Teresa
Alves, Vera
Mota-Pinto, Anabela
Sarmento-Ribeiro, Ana Bela
dc.subject.por.fl_str_mv Stresse Oxidativo
Cancro da próstata
topic Stresse Oxidativo
Cancro da próstata
description Oxidative stress has been associated with prostate cancer development and progression due to an increase of reactive oxygen species (ROS). However, the mechanisms whereby ROS and the antioxidant system participate in cancer progression remain unclear. In order to clarify the influence of oxidative stress in prostate cancer progression, we performed this study in two human prostate cancer cell lines, PC3 and HPV10 (from metastasis and from localized cancer, respectively) and RWPE1 cells derived from normal prostate epithelium. Cells were treated with hydrogen peroxide (H2O2) and PC3 cells were also treated with diethyl maleate (DEM). The effect on cell growth, viability, mitochondria membrane potential and oxidative stress was analysed. Oxidative stress was evaluated based on ROS production, oxidative lesion of lipids (MDA) and on determination of antioxidants, including enzyme activity of glutathione peroxidase (Gl-Px), glutathione reductase (Gl-Red) and on the quantification of glutathione (GSH), glutathione-s-transferase (GST) and total antioxidant status (TAS). PC3 shows higher ROS production but also the highest GSH levels and Gl-Red activity, possibly contributing to oxidative stress resistance. This is also associated with higher mitochondrial membrane potential, TAS and lower lipid peroxidation. On the other hand, we identified Gl-Red activity reduction as a new strategy in overcoming oxidative stress resistance, by inducing H2O2 cytotoxicity. Therefore these results suggest Gl-Red activity reduction as a new potential therapeutic approach, in prostate cancer.
publishDate 2012
dc.date.none.fl_str_mv 2012
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10316/20296
http://hdl.handle.net/10316/20296
https://doi.org/10.1016/j.fob.2012.05.001
url http://hdl.handle.net/10316/20296
https://doi.org/10.1016/j.fob.2012.05.001
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv http://www.sciencedirect.com/science/article/pii/S2211546312000186
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
_version_ 1799133708542803968