Effects of additives on the activity of a covalently immobilised lipase in organic media
Autor(a) principal: | |
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Data de Publicação: | 1998 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10316/3843 https://doi.org/10.1016/S0168-1656(98)00157-6 |
Resumo: | Lipase from Mucor miehei was covalently immobilised onto the graft copolymer poly(ethylene)-g.co-hydroxyethyl methacrylate (PE-g.co-HEMA), partially hydrolysed, via a spacer arm of 1,6-diaminohexane activated with glutaraldehyde. To improve the lipolytic activity of the immobilised lipase (for the synthesis of isoamyl-caprylate, as a model), the effect of several additives was investigated. Polyethylene glycol (PEG), glutaraldehyde, organic solvents and buffers, were added during the immobilisation procedure and their effects are reported and compared with the behaviour of the lipolytic preparation without pre-treatment. An increase of 40-100% in the activity was obtained when small quantities of PEG 2000 and glutaraldehyde (used also as an activator of the spacer arm) were added. The activity had a maximum when the pH of the lipase attachment solution was 7.2 and buffered with phosphate. The effect of the aggregation level of biocatalyst particles on the amount of water retained, as well as the effect of the immobilisation on solid supports on the stability to organic solvents, is also reported. |
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Effects of additives on the activity of a covalently immobilised lipase in organic mediaLipase from Mucor miehei was covalently immobilised onto the graft copolymer poly(ethylene)-g.co-hydroxyethyl methacrylate (PE-g.co-HEMA), partially hydrolysed, via a spacer arm of 1,6-diaminohexane activated with glutaraldehyde. To improve the lipolytic activity of the immobilised lipase (for the synthesis of isoamyl-caprylate, as a model), the effect of several additives was investigated. Polyethylene glycol (PEG), glutaraldehyde, organic solvents and buffers, were added during the immobilisation procedure and their effects are reported and compared with the behaviour of the lipolytic preparation without pre-treatment. An increase of 40-100% in the activity was obtained when small quantities of PEG 2000 and glutaraldehyde (used also as an activator of the spacer arm) were added. The activity had a maximum when the pH of the lipase attachment solution was 7.2 and buffered with phosphate. The effect of the aggregation level of biocatalyst particles on the amount of water retained, as well as the effect of the immobilisation on solid supports on the stability to organic solvents, is also reported.http://www.sciencedirect.com/science/article/B6T3C-3V8CG7H-7/1/f8c9c7095fea93b2ccae17922a1f89d61998info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleaplication/PDFhttp://hdl.handle.net/10316/3843http://hdl.handle.net/10316/3843https://doi.org/10.1016/S0168-1656(98)00157-6engJournal of Biotechnology. 66:1 (1998) 61-67Rocha, J. M. S.Gil, M. H.Garcia, F. A. P.info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2020-11-06T16:59:38Zoai:estudogeral.uc.pt:10316/3843Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T20:59:16.876210Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Effects of additives on the activity of a covalently immobilised lipase in organic media |
title |
Effects of additives on the activity of a covalently immobilised lipase in organic media |
spellingShingle |
Effects of additives on the activity of a covalently immobilised lipase in organic media Rocha, J. M. S. |
title_short |
Effects of additives on the activity of a covalently immobilised lipase in organic media |
title_full |
Effects of additives on the activity of a covalently immobilised lipase in organic media |
title_fullStr |
Effects of additives on the activity of a covalently immobilised lipase in organic media |
title_full_unstemmed |
Effects of additives on the activity of a covalently immobilised lipase in organic media |
title_sort |
Effects of additives on the activity of a covalently immobilised lipase in organic media |
author |
Rocha, J. M. S. |
author_facet |
Rocha, J. M. S. Gil, M. H. Garcia, F. A. P. |
author_role |
author |
author2 |
Gil, M. H. Garcia, F. A. P. |
author2_role |
author author |
dc.contributor.author.fl_str_mv |
Rocha, J. M. S. Gil, M. H. Garcia, F. A. P. |
description |
Lipase from Mucor miehei was covalently immobilised onto the graft copolymer poly(ethylene)-g.co-hydroxyethyl methacrylate (PE-g.co-HEMA), partially hydrolysed, via a spacer arm of 1,6-diaminohexane activated with glutaraldehyde. To improve the lipolytic activity of the immobilised lipase (for the synthesis of isoamyl-caprylate, as a model), the effect of several additives was investigated. Polyethylene glycol (PEG), glutaraldehyde, organic solvents and buffers, were added during the immobilisation procedure and their effects are reported and compared with the behaviour of the lipolytic preparation without pre-treatment. An increase of 40-100% in the activity was obtained when small quantities of PEG 2000 and glutaraldehyde (used also as an activator of the spacer arm) were added. The activity had a maximum when the pH of the lipase attachment solution was 7.2 and buffered with phosphate. The effect of the aggregation level of biocatalyst particles on the amount of water retained, as well as the effect of the immobilisation on solid supports on the stability to organic solvents, is also reported. |
publishDate |
1998 |
dc.date.none.fl_str_mv |
1998 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10316/3843 http://hdl.handle.net/10316/3843 https://doi.org/10.1016/S0168-1656(98)00157-6 |
url |
http://hdl.handle.net/10316/3843 https://doi.org/10.1016/S0168-1656(98)00157-6 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal of Biotechnology. 66:1 (1998) 61-67 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
aplication/PDF |
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reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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