A comparative genomic analysis of energy metabolism in sulfate reducing bacteria and archaea

Detalhes bibliográficos
Autor(a) principal: Pereira, Ines Antunes
Data de Publicação: 2011
Outros Autores: Venceslau, S.S., Ramos, Ana Raquel, da Silva, Sofia Isabel Marques, Grein, Fabian
Tipo de documento: Artigo
Idioma: und
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/80310
Resumo: The number of sequenced genomes of sulfate-reducing organisms (SRO) has increased significantly in the recent years, providing an opportunity for a broader perspective into the energy metabolism of such organisms. In this work we carried out a comparative survey of energy metabolism genes found in twenty-five available genomes of SRO. This analysis revealed a higher diversity of possible energy conserving pathways than classically considered to be present in these organisms, and permitted the identification of new proteins not known to be present in this group. The Deltaproteobacteria (and Thermodesulfovibrio yellowstonii) are characterized by a large number of cytochromes c and cytochrome c-associated membrane redox complexes, indicating that periplasmic electron transfer pathways are important in these bacteria. The Archaea and Clostridia groups contain practically no cytochromes c or associated membrane complexes. However, despite the absence of a periplasmic space, a few extracytoplasmic membrane redox proteins were detected in the Gram-positive bacteria. Several ion-translocating complexes were detected in SRO including H+-pyrophosphatases, complex I homologues, Rnf and Ech/Coo hydrogenases. Furthermore, we found evidence that cytoplasmic electron bifurcating mechanisms, recently described for other anaerobes, are also likely to play an important role in energy metabolism of SRO. A number of cytoplasmic [NiFe] and [FeFe] hydrogenases, formate dehydrogenases and heterodisulfide reductase-related proteins are likely candidates to be involved in energy coupling through electron bifurcation, from diverse electron donors such as H2, formate, pyruvate, NAD(P)H, ?-oxidation and others. In conclusion, this analysis indicates that energy metabolism of SRO is far more versatile than previously considered, and that both chemiosmotic and flavin-based electron bifurcating mechanisms provide alternative strategies for energy conservation.
id RCAP_250a770112be2b2674d26cca387cd49d
oai_identifier_str oai:run.unl.pt:10362/80310
network_acronym_str RCAP
network_name_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository_id_str 7160
spelling A comparative genomic analysis of energy metabolism in sulfate reducing bacteria and archaeaSDG 7 - Affordable and Clean EnergyThe number of sequenced genomes of sulfate-reducing organisms (SRO) has increased significantly in the recent years, providing an opportunity for a broader perspective into the energy metabolism of such organisms. In this work we carried out a comparative survey of energy metabolism genes found in twenty-five available genomes of SRO. This analysis revealed a higher diversity of possible energy conserving pathways than classically considered to be present in these organisms, and permitted the identification of new proteins not known to be present in this group. The Deltaproteobacteria (and Thermodesulfovibrio yellowstonii) are characterized by a large number of cytochromes c and cytochrome c-associated membrane redox complexes, indicating that periplasmic electron transfer pathways are important in these bacteria. The Archaea and Clostridia groups contain practically no cytochromes c or associated membrane complexes. However, despite the absence of a periplasmic space, a few extracytoplasmic membrane redox proteins were detected in the Gram-positive bacteria. Several ion-translocating complexes were detected in SRO including H+-pyrophosphatases, complex I homologues, Rnf and Ech/Coo hydrogenases. Furthermore, we found evidence that cytoplasmic electron bifurcating mechanisms, recently described for other anaerobes, are also likely to play an important role in energy metabolism of SRO. A number of cytoplasmic [NiFe] and [FeFe] hydrogenases, formate dehydrogenases and heterodisulfide reductase-related proteins are likely candidates to be involved in energy coupling through electron bifurcation, from diverse electron donors such as H2, formate, pyruvate, NAD(P)H, ?-oxidation and others. In conclusion, this analysis indicates that energy metabolism of SRO is far more versatile than previously considered, and that both chemiosmotic and flavin-based electron bifurcating mechanisms provide alternative strategies for energy conservation.Instituto de Tecnologia Química e Biológica António Xavier (ITQB)RUNPereira, Ines AntunesVenceslau, S.S.Ramos, Ana Raquelda Silva, Sofia Isabel MarquesGrein, Fabian2019-09-06T22:04:29Z2011-01-012011-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10362/80310und1664-302XPURE: 257455https://doi.org/10.3389/fmicb.2011.00069info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T04:35:34Zoai:run.unl.pt:10362/80310Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:35:52.677192Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv A comparative genomic analysis of energy metabolism in sulfate reducing bacteria and archaea
title A comparative genomic analysis of energy metabolism in sulfate reducing bacteria and archaea
spellingShingle A comparative genomic analysis of energy metabolism in sulfate reducing bacteria and archaea
Pereira, Ines Antunes
SDG 7 - Affordable and Clean Energy
title_short A comparative genomic analysis of energy metabolism in sulfate reducing bacteria and archaea
title_full A comparative genomic analysis of energy metabolism in sulfate reducing bacteria and archaea
title_fullStr A comparative genomic analysis of energy metabolism in sulfate reducing bacteria and archaea
title_full_unstemmed A comparative genomic analysis of energy metabolism in sulfate reducing bacteria and archaea
title_sort A comparative genomic analysis of energy metabolism in sulfate reducing bacteria and archaea
author Pereira, Ines Antunes
author_facet Pereira, Ines Antunes
Venceslau, S.S.
Ramos, Ana Raquel
da Silva, Sofia Isabel Marques
Grein, Fabian
author_role author
author2 Venceslau, S.S.
Ramos, Ana Raquel
da Silva, Sofia Isabel Marques
Grein, Fabian
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Instituto de Tecnologia Química e Biológica António Xavier (ITQB)
RUN
dc.contributor.author.fl_str_mv Pereira, Ines Antunes
Venceslau, S.S.
Ramos, Ana Raquel
da Silva, Sofia Isabel Marques
Grein, Fabian
dc.subject.por.fl_str_mv SDG 7 - Affordable and Clean Energy
topic SDG 7 - Affordable and Clean Energy
description The number of sequenced genomes of sulfate-reducing organisms (SRO) has increased significantly in the recent years, providing an opportunity for a broader perspective into the energy metabolism of such organisms. In this work we carried out a comparative survey of energy metabolism genes found in twenty-five available genomes of SRO. This analysis revealed a higher diversity of possible energy conserving pathways than classically considered to be present in these organisms, and permitted the identification of new proteins not known to be present in this group. The Deltaproteobacteria (and Thermodesulfovibrio yellowstonii) are characterized by a large number of cytochromes c and cytochrome c-associated membrane redox complexes, indicating that periplasmic electron transfer pathways are important in these bacteria. The Archaea and Clostridia groups contain practically no cytochromes c or associated membrane complexes. However, despite the absence of a periplasmic space, a few extracytoplasmic membrane redox proteins were detected in the Gram-positive bacteria. Several ion-translocating complexes were detected in SRO including H+-pyrophosphatases, complex I homologues, Rnf and Ech/Coo hydrogenases. Furthermore, we found evidence that cytoplasmic electron bifurcating mechanisms, recently described for other anaerobes, are also likely to play an important role in energy metabolism of SRO. A number of cytoplasmic [NiFe] and [FeFe] hydrogenases, formate dehydrogenases and heterodisulfide reductase-related proteins are likely candidates to be involved in energy coupling through electron bifurcation, from diverse electron donors such as H2, formate, pyruvate, NAD(P)H, ?-oxidation and others. In conclusion, this analysis indicates that energy metabolism of SRO is far more versatile than previously considered, and that both chemiosmotic and flavin-based electron bifurcating mechanisms provide alternative strategies for energy conservation.
publishDate 2011
dc.date.none.fl_str_mv 2011-01-01
2011-01-01T00:00:00Z
2019-09-06T22:04:29Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/80310
url http://hdl.handle.net/10362/80310
dc.language.iso.fl_str_mv und
language und
dc.relation.none.fl_str_mv 1664-302X
PURE: 257455
https://doi.org/10.3389/fmicb.2011.00069
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
_version_ 1799137979094007808