Protein crystallization in a microfluidic contactor with nafion®117 membranes
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2021 |
| Outros Autores: | , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
| Texto Completo: | http://hdl.handle.net/10362/133123 |
Resumo: | UIDB/50006/2020 UIDP/50006/2020 “Erasmus Mundus Doctorate in Membrane Engineering”–EUDIME (FPA 2011–2014, http://www.eudime.unical.it |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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7160 |
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Protein crystallization in a microfluidic contactor with nafion®117 membranesMembrane contactorsNafion membraneProtein crystallizationProtein structureSolute diffusionChemical Engineering (miscellaneous)Process Chemistry and TechnologyFiltration and SeparationUIDB/50006/2020 UIDP/50006/2020 “Erasmus Mundus Doctorate in Membrane Engineering”–EUDIME (FPA 2011–2014, http://www.eudime.unical.itProtein crystallization still remains mostly an empirical science, as the production of crystals with the required quality for X-ray analysis is dependent on the intensive screening of the best protein crystallization and crystal’s derivatization conditions. Herein, this demanding step was addressed by the development of a high-throughput and low-budget microfluidic platform consisting of an ion exchange membrane (117 Nafion® membrane) sandwiched between a channel layer (stripping phase compartment) and a wells layer (feed phase compartment) forming 75 independent microcontactors. This microfluidic device allows for a simultaneous and independent screening of multiple protein crystallization and crystal derivatization conditions, using Hen EggWhite Lysozyme (HEWL) as the model protein and Hg2+ as the derivatizing agent. This microdevice offers well-regulated crystallization and subsequent crystal derivatization processes based on the controlled transport of water and ions provided by the 117 Nafion® membrane. Diffusion coefficients of water and the derivatizing agent (Hg2+) were evaluated, showing the positive influence of the protein drop volume on the number of crystals and crystal size. This microfluidic system allowed for crystals with good structural stability and high X-ray diffraction quality and, thus, it is regarded as an efficient tool that may contribute to the enhancement of the proteins’ crystals structural resolution.LAQV@REQUIMTEDQ - Departamento de QuímicaUCIBIO - Applied Molecular Biosciences UnitRUNPolino, MariellaRho, Hoon-sukPina, M. P.Mallada, ReysCarvalho, Ana LuísaRomão, Maria João C.Coelhoso, IsabelGardeniers, J. G.E.Crespo, João GoulãoPortugal, Carla A. M.2022-02-17T23:19:23Z2021-082021-08-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10362/133123eng0076-6356PURE: 36672585https://doi.org/10.3390/membranes11080549info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T05:11:47Zoai:run.unl.pt:10362/133123Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:47:42.408886Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
| dc.title.none.fl_str_mv |
Protein crystallization in a microfluidic contactor with nafion®117 membranes |
| title |
Protein crystallization in a microfluidic contactor with nafion®117 membranes |
| spellingShingle |
Protein crystallization in a microfluidic contactor with nafion®117 membranes Polino, Mariella Membrane contactors Nafion membrane Protein crystallization Protein structure Solute diffusion Chemical Engineering (miscellaneous) Process Chemistry and Technology Filtration and Separation |
| title_short |
Protein crystallization in a microfluidic contactor with nafion®117 membranes |
| title_full |
Protein crystallization in a microfluidic contactor with nafion®117 membranes |
| title_fullStr |
Protein crystallization in a microfluidic contactor with nafion®117 membranes |
| title_full_unstemmed |
Protein crystallization in a microfluidic contactor with nafion®117 membranes |
| title_sort |
Protein crystallization in a microfluidic contactor with nafion®117 membranes |
| author |
Polino, Mariella |
| author_facet |
Polino, Mariella Rho, Hoon-suk Pina, M. P. Mallada, Reys Carvalho, Ana Luísa Romão, Maria João C. Coelhoso, Isabel Gardeniers, J. G.E. Crespo, João Goulão Portugal, Carla A. M. |
| author_role |
author |
| author2 |
Rho, Hoon-suk Pina, M. P. Mallada, Reys Carvalho, Ana Luísa Romão, Maria João C. Coelhoso, Isabel Gardeniers, J. G.E. Crespo, João Goulão Portugal, Carla A. M. |
| author2_role |
author author author author author author author author author |
| dc.contributor.none.fl_str_mv |
LAQV@REQUIMTE DQ - Departamento de Química UCIBIO - Applied Molecular Biosciences Unit RUN |
| dc.contributor.author.fl_str_mv |
Polino, Mariella Rho, Hoon-suk Pina, M. P. Mallada, Reys Carvalho, Ana Luísa Romão, Maria João C. Coelhoso, Isabel Gardeniers, J. G.E. Crespo, João Goulão Portugal, Carla A. M. |
| dc.subject.por.fl_str_mv |
Membrane contactors Nafion membrane Protein crystallization Protein structure Solute diffusion Chemical Engineering (miscellaneous) Process Chemistry and Technology Filtration and Separation |
| topic |
Membrane contactors Nafion membrane Protein crystallization Protein structure Solute diffusion Chemical Engineering (miscellaneous) Process Chemistry and Technology Filtration and Separation |
| description |
UIDB/50006/2020 UIDP/50006/2020 “Erasmus Mundus Doctorate in Membrane Engineering”–EUDIME (FPA 2011–2014, http://www.eudime.unical.it |
| publishDate |
2021 |
| dc.date.none.fl_str_mv |
2021-08 2021-08-01T00:00:00Z 2022-02-17T23:19:23Z |
| dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10362/133123 |
| url |
http://hdl.handle.net/10362/133123 |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
| dc.relation.none.fl_str_mv |
0076-6356 PURE: 36672585 https://doi.org/10.3390/membranes11080549 |
| dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
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openAccess |
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application/pdf |
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reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799138079537102848 |